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CHCHD3 Antibody – Goat Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS422299

MBS422299 is an antibody targeting CHCHD3. Also known as Goat anti-CHCHD3 (aa151-164) Antibody. Reactive in Tested: Human; Expected from sequence similarity: Human, Mouse, Rat, Dog, Cow, Sheep. Host: Goat. More details

$450 / 0.1 mg +1 FREE 8GB USB
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Sample Manual Insert View PDF
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Testing Data View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Product Overview

Online Datasheet in PDF
Product Name
CHCHD3, Polyclonal Antibody
Full Product Name
Goat anti-CHCHD3 (aa151-164) Antibody
Product Gene Name
Product Synonym Names
Goat Anti-CHCHD3 Antibody; CHCHD3; coiled-coil-helix-coiled-coil-helix domain containing 3; FLJ20420; coiled-coil-helix-coiled-coil-helix domain containing 3 antibody; FLJ20420 antibody; CHCHD3 antibody; CHCHD3 (aa151-164)
Immunogen Sequence
RSSEFYRVTT EQYQ
Clonality
Polyclonal
Host
Goat
Species Reactivity
Tested: Human; Expected from sequence similarity: Human, Mouse, Rat, Dog, Cow, Sheep
Form/Format
Supplied at 0.5 mg/ml in Tris saline, 0. 02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Purity/Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Concentration
100ug specific antibody in 200ul (lot specific)
Preparation and Storage
Aliquot and store at -20 degree C. Minimize freezing and thawing.
Antibody/Peptide Pairs
CHCHD3 peptide (MBS427208) is used for blocking the activity of CHCHD3 antibody (MBS422299)
Molecular Weight
26,152 Da
OMIM
613748
Immunogen
Peptide with sequence C-RSSEFYRVTTEQYQ, from the internal region of the protein sequence according to NP_060282.1.
Epitope
Internal region
ISO Certification
Manufactured in an ISO 9001:2008 Certified Laboratory.
Applications Tested/Suitable for anti-CHCHD3 antibody
Peptide ELISA (EIA), Western Blot (WB)
Application Notes for anti-CHCHD3 antibody
Peptide ELISA: Antibody detection limit dilution 1: 32000.
Western Blot: Approx 26.2kDa band observed in Human Placenta lysates (calculated MW of 26kDa according to NP_060282.1). Recommended concentration: 0.3-1ug/ml. Anonymous customer reported ~30kDa in THP1 and in human primary monocyte derived dendritic cells.

Western Blot (WB) of anti-CHCHD3 antibody

(0.3ug/ml) staining of Human Placenta lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Goat Anti-CHCHD3 Polyclonal Antibody – Cat# MBS422299

Other Notes

Small volumes of anti-CHCHD3 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for CHCHD3. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
MICOS complex subunit MIC19 isoform 2
NCBI Official Synonym Full Names
coiled-coil-helix-coiled-coil-helix domain containing 3
NCBI Official Symbol
CHCHD3
NCBI Official Synonym Symbols
Mic19; MINOS3; PPP1R22
UniProt Protein Name
MICOS complex subunit MIC19
UniProt Synonym Protein Names
Coiled-coil-helix-coiled-coil-helix domain-containing protein 3
UniProt Gene Name
CHCHD3
UniProt Synonym Gene Names
MIC19; MINOS3
UniProt Entry Name
MIC19_HUMAN
NCBI Protein Information
MICOS complex subunit MIC19

NCBI Summary for CHCHD3

The protein encoded by this gene is an inner mitochondrial membrane scaffold protein. Absence of the encoded protein affects the structural integrity of mitochondrial cristae and leads to reductions in ATP production, cell growth, and oxygen consumption. This protein is part of the mitochondrial contact site and cristae organizing system (MICOS). Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Nov 2015]
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UniProt Comments for CHCHD3

CHCHD3: May be a scaffolding protein that stabilizes protein complexes involved in maintaining mitochondrial crista architecture and protein import.

Protein type: Mitochondrial

Chromosomal Location of Human Ortholog: 7q33

Cellular Component: cytoplasm; mitochondrial inner membrane; mitochondrion; nucleus

Molecular Function: phosphatase binding; protein binding; protein complex scaffold

Biological Process: cristae formation; inner mitochondrial membrane organization and biogenesis; mitochondrial fusion; negative regulation of transcription from RNA polymerase II promoter; transcription, DNA-dependent
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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