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HSH2D Antibody – Goat Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS422324

MBS422324 is an antibody targeting HSH2D. Also known as Goat anti-HSH2D Antibody. Reactive in Tested: Human; Expected from sequence similarity: Human, Mouse. Host: Goat. More details

$450 / 0.1 mg +1 FREE 8GB USB
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Sample Manual Insert View PDF
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Testing Data View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Product Overview

Online Datasheet in PDF
Product Name
HSH2D, Polyclonal Antibody
Full Product Name
Goat anti-HSH2D Antibody
Product Gene Name
Product Synonym Names
Goat Anti-HSH2D (aa325-339) Antibody; HSH2D; hematopoietic SH2 domain containing; ALX; FLJ14886; HSH2; adaptor in lymphocytes of unknown function X; hematopoietic SH2 protein; adaptor in lymphocytes of unknown function X antibody; ALX antibody; FLJ14886 antibody; hematopoietic SH2 domain containing antibody; hematopoietic SH2 protein antibody; HSH2 antibody; HSH2D antibody
Immunogen Sequence
HSHVGYTLSY KAQ
Clonality
Polyclonal
Host
Goat
Species Reactivity
Tested: Human; Expected from sequence similarity: Human, Mouse
Form/Format
Supplied at 0.5 mg/ml in Tris saline, 0. 02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Purity/Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Concentration
100ug specific antibody in 200ul (lot specific)
Preparation and Storage
Aliquot and store at -20 degree C. Minimize freezing and thawing.
Antibody/Peptide Pairs
HSH2D peptide (MBS428433) is used for blocking the activity of HSH2D antibody (MBS422324)
Molecular Weight
32,704 Da
OMIM
608349
Immunogen
Peptide with sequence C-HSHVGYTLSYKAQ, from the internal region of the protein sequence according to NP_116244.1.
Epitope
Internal region
ISO Certification
Manufactured in an ISO 9001:2008 Certified Laboratory.
Applications Tested/Suitable for anti-HSH2D antibody
Peptide ELISA (EIA), Western Blot (WB)
Application Notes for anti-HSH2D antibody
Peptide ELISA: Antibody detection limit dilution 1: 4000.
Western Blot: Approx 37kDa band observed in lysates of cell line K562 (calculated MW of 39. 0kDa according to NP_116244.1). Recommended concentration: 0.3-1ug/ml.

Western Blot (WB) of anti-HSH2D antibody

(0.3ug/ml) staining of K562 lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Goat Anti-HSH2D Polyclonal Antibody – Cat# MBS422324

Other Notes

Small volumes of anti-HSH2D antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for HSH2D. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Secondary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
hematopoietic SH2 domain-containing protein isoform 1
NCBI Official Synonym Full Names
hematopoietic SH2 domain containing
NCBI Official Symbol
HSH2D
NCBI Official Synonym Symbols
ALX; HSH2
UniProt Protein Name
Hematopoietic SH2 domain-containing protein
UniProt Synonym Protein Names
Adaptor in lymphocytes of unknown function X
UniProt Gene Name
HSH2D
UniProt Synonym Gene Names
ALX; Hematopoietic SH2 protein
UniProt Entry Name
HSH2D_HUMAN
NCBI Protein Information
hematopoietic SH2 domain-containing protein

NCBI Summary for HSH2D

T-cell activation requires 2 signals: recognition of antigen by the T-cell receptor (see TCR; MIM 186880) and a costimulatory signal provided primarily by CD28 (MIM 186760) in naive T cells. HSH2 is a target of both of these signaling pathways (Greene et al., 2003 [PubMed 12960172]).[supplied by OMIM, Mar 2008]
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UniProt Comments for HSH2D

HSH2: May be a modulator of the apoptotic response through its ability to affect mitochondrial stability. Adapter protein involved in tyrosine kinase and CD28 signaling. Seems to affect CD28-mediated activation of the RE/AP element of the interleukin-2 promoter. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Adaptor/scaffold

Chromosomal Location of Human Ortholog: 19p13.12

Cellular Component: cytosol; mitochondrion; nucleus

Molecular Function: protein binding; SH3/SH2 adaptor activity

Biological Process: negative regulation of B cell apoptosis; negative regulation of mitochondrial depolarization; positive regulation of signal transduction; T cell activation
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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