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CPLA2-Zeta (PLA2G4F) Antibody – Goat Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS422352

MBS422352 is an antibody targeting PLA2G4F. Also known as Goat anti-CPLA2-zeta Antibody. Reactive in Expected from sequence similarity: Human, Mouse, Rat, Cow. Host: Goat. More details

$305 / 0.1 mg +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
CPLA2-zeta (PLA2G4F), Polyclonal Antibody
Full Product Name
Goat anti-CPLA2-zeta Antibody
Product Gene Name
anti-PLA2G4F antibody
Product Synonym Gene Name
CPLA2-zeta
Product Synonym Names
PLA2G4F; phospholipase A2, group IVF; DKFZp666G192; DKFZp781B229; PLA2G4FZ; cytosolic phospholipase A2 zeta; cPLA2-zeta; cPLA2-zeta antibody; cytosolic phospholipase A2 zeta antibody; DKFZp666G192 antibody; DKFZp781B229 antibody; phospholipase A2; group IVF antibody; PLA2G4FZ antibody; PLA2G4F antibody; CPLA2-zeta
Immunogen Sequence
QTAEEKAFGD FVINR
Clonality
Polyclonal
Host
Goat
Species Reactivity
Expected from sequence similarity: Human, Mouse, Rat, Cow
Form/Format
Supplied at 0.5 mg/ml in Tris saline, 0. 02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Purity/Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Concentration
100ug specific antibody in 200ul (lot specific)
Preparation and Storage
Aliquot and store at -20 degree C. Minimize freezing and thawing.
Antibody/Peptide Pairs
CPLA2-zeta peptide (MBS427282) is used for blocking the activity of CPLA2-zeta antibody (MBS422352)
Molecular Weight
95,310 Da
Immunogen
Peptide with sequence C-QTAEEKAFGDFVINR, from the internal region (near C Terminus) of the protein sequence according to NP_998765.3.
Epitope
Internal region (near C Terminus)
ISO Certification
Manufactured in an ISO 9001:2008 Certified Laboratory.
Applications Tested/Suitable for anti-PLA2G4F antibody
Peptide ELISA (EIA)
Application Notes for anti-PLA2G4F antibody
Peptide ELISA: Antibody detection limit dilution 1: 16000.
Western Blot: Preliminary experiments gave bands at approx 55-60kDa and 35kDa in Human Placenta lysates after 1ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the bands we observe given the calculated size of 95.1kDa according to NP_998765.3. Both detected bands were successfully blocked by incubation with the immunizing peptide (and BLAST results with the immunizing peptide sequence did not identify any other proteins to explain the additional bands).

Other Notes

Small volumes of anti-PLA2G4F antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for PLA2G4F. It may not necessarily be applicable to this product.

NCBI GI #
281371376
NCBI GeneID
255189
NCBI Accession #
NP_998765.3
UniProt Secondary Accession #
Q6ZMC8
UniProt Related Accession #
Q68DD2
GenBank Nucleotide #
NM_213600.3
NCBI Official Full Name
cytosolic phospholipase A2 zeta
NCBI Official Synonym Full Names
phospholipase A2 group IVF
NCBI Official Symbol
PLA2G4F
NCBI Official Synonym Symbols
PLA2G4FZ
UniProt Protein Name
Cytosolic phospholipase A2 zeta
UniProt Synonym Protein Names
Phospholipase A2 group IVF
Protein Family
Cytosolic phospholipase
UniProt Gene Name
PLA2G4F
UniProt Synonym Gene Names
cPLA2-zeta
UniProt Entry Name
PA24F_HUMAN
NCBI Protein Information
cytosolic phospholipase A2 zeta

UniProt Comments for PLA2G4F

PLA2G4F: a calcium-dependent phospholipase A2 that catalyzes the release of arachidonic acid from membrane phospholipids. Selectively hydrolyzes glycerophospholipids in the sn-2 position. Has higher enzyme activity for phosphatidylethanolamine than phosphatidylcholine. Has a much higher lysophospholipase and PLA(2) activity than PLA2G4B. Potently inhibited by pyrrolidine-2 and Wyeth-1. Its specific activity, inhibitor sensitivity, and low micromolar calcium dependence are similar to PLA2G4A. Translocates to ruffles and dynamic vesicular structures in a calcium-dependent manner. The N-terminal C2 domain associates with lipid membranes and mediates its regulation by presenting the active site to its substrate in response to elevations of cytosolic Ca2+ Three isoforms of the human protein are produced by alternative splicing.

Protein type: EC 3.1.1.4; Phospholipase; Lipid Metabolism - arachidonic acid

Chromosomal Location of Human Ortholog: 15q15.1

Cellular Component: cytosol; lysosomal membrane; vesicle

Molecular Function: calcium-dependent phospholipase A2 activity; lysophospholipase activity; metal ion binding; phospholipase A1 activity; phospholipase A2 activity

Biological Process: arachidonic acid secretion; phospholipid catabolic process; phospholipid metabolic process; prostaglandin biosynthetic process
Show more

Research Articles on PLA2G4F

1. Observational study of gene-disease association, gene-environment interaction, and pharmacogenomic / toxicogenomic. (HuGE Navigator)

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Pathways associated with anti-PLA2G4F antibody

 Products by Pathway  Pathway Diagram
 Acyl Chain Remodelling Of PC Pathway antibodies  Acyl Chain Remodelling Of PC Pathway Diagram
 Acyl Chain Remodelling Of PE Pathway antibodies  Acyl Chain Remodelling Of PE Pathway Diagram
 Acyl Chain Remodelling Of PG Pathway antibodies  Acyl Chain Remodelling Of PG Pathway Diagram
 Acyl Chain Remodelling Of PI Pathway antibodies  Acyl Chain Remodelling Of PI Pathway Diagram
 Acyl Chain Remodelling Of PS Pathway antibodies  Acyl Chain Remodelling Of PS Pathway Diagram
 Arachidonic Acid Metabolism Pathway antibodies  Arachidonic Acid Metabolism Pathway Diagram
 Arachidonic Acid Metabolism Pathway antibodies  Arachidonic Acid Metabolism Pathway Diagram
 Choline Metabolism In Cancer Pathway antibodies  Choline Metabolism In Cancer Pathway Diagram
 Choline Metabolism In Cancer Pathway antibodies  Choline Metabolism In Cancer Pathway Diagram
 Ether Lipid Metabolism Pathway antibodies  Ether Lipid Metabolism Pathway Diagram

Diseases associated with anti-PLA2G4F antibody

 Disease Name  Pubmed Publications
 Disease Progression Antibodies  >1 publications with PLA2G4F and Disease Progression

Organs/Tissues associated with anti-PLA2G4F antibody

 Organ/Tissue Name  Pubmed Publications
 Lung Antibodies  >2 publications with PLA2G4F and Lung
 Intestine Antibodies  >2 publications with PLA2G4F and Intestine
 Mouth Antibodies  >1 publications with PLA2G4F and Mouth
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