Goat Anti-POU2F3 Polyclonal Antibody – Cat# MBS422362

(2ug/ml) staining of HEK293 lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

POU2F3 Antibody – Goat Polyclonal

Name: POU2F3 Antibody; Polyclonal; Goat; Human-Reactive; MyBioSource
Brand: MyBioSource
SKU: MBS422362
Price: 450 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS422362

MBS422362 is an antibody targeting POU2F3. Also known as Goat anti-POU2F3 Antibody. Reactive in Tested: Human; Expected from sequence similarity: Human, Rat, Dog, Cow. Host: Goat. More details

$450 / 0.1 mg +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Online Datasheet in PDF

Product Name

POU2F3, Polyclonal Antibody

Full Product Name

Goat anti-POU2F3 Antibody

Product Gene Name

anti-POU2F3 antibody

Product Synonym Names

POU2F3; POU class 2 homeobox 3; Epoc-1; FLJ40063; MGC126698; OCT11; PLA-1; Skn-1a; POU domain, class 2, transcription factor 3; POU transcription factor; Epoc-1 antibody; FLJ40063 antibody; MGC126698 antibody; OCT11 antibody; PLA-1 antibody; POU class 2 homeobox 3 antibody; POU domain; class 2; transcription factor 3 antibody; POU transcription factor antibody; Skn-1a antibody; POU2F3 antibody

Immunogen Sequence

EPHLEASQHL PVPKH

Clonality

Polyclonal

Host

Goat

Species Reactivity

Tested: Human; Expected from sequence similarity: Human, Rat, Dog, Cow

Form/Format

Supplied at 0.5 mg/ml in Tris saline, 0. 02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

Purity/Purification

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Concentration

100ug specific antibody in 200ul (lot specific)

Preparation and Storage

Aliquot and store at -20 degree C. Minimize freezing and thawing.

Antibody/Peptide Pairs

POU2F3 peptide (MBS427294) is used for blocking the activity of POU2F3 antibody (MBS422362)

Molecular Weight

46,949 Da

OMIM

607394

Immunogen

Peptide with sequence C-EPHLEASQHLPVPKH, from the internal region of the protein sequence according to NP_055167.2.

Epitope

Internal region

ISO Certification

Manufactured in an ISO 9001:2008 Certified Laboratory.

Applications Tested/Suitable for anti-POU2F3 antibody

Peptide ELISA (EIA), Western Blot (WB)

Application Notes for anti-POU2F3 antibody

Peptide ELISA: Antibody detection limit dilution 1: 32000.
Western Blot: Approx 50kDa band observed in lysates of cell lines HEK293 and HepG2 (calculated MW of 47.4kDa according to NP_055167.2). Recommended concentration: 1-3ug/ml.

Other Notes

Small volumes of anti-POU2F3 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for POU2F3. It may not necessarily be applicable to this product.

NCBI GI #

148664218

NCBI GeneID

25833

NCBI Accession #

NP_055167.2

UniProt Secondary Accession #

Q3MIY3; Q9UKR7; Q9Y504; A8K7H8; B4DY07; F5GWW6

UniProt Related Accession #

Q9UKI9

GenBank Nucleotide #

NM_014352.3

NCBI Official Full Name

POU domain, class 2, transcription factor 3 isoform 1

NCBI Official Synonym Full Names

POU class 2 homeobox 3

NCBI Official Symbol

POU2F3

NCBI Official Synonym Symbols

PLA1; OCT11; PLA-1; Epoc-1; OCT-11; OTF-11; Skn-1a

UniProt Protein Name

POU domain, class 2, transcription factor 3

UniProt Synonym Protein Names

Octamer-binding protein 11; Oct-11; Octamer-binding transcription factor 11; OTF-11; Transcription factor PLA-1; Transcription factor Skn-1

Protein Family

POU domain, class 2, transcription factor

UniProt Gene Name

POU2F3

UniProt Synonym Gene Names

OTF11; PLA1; Oct-11; OTF-11

UniProt Entry Name

PO2F3_HUMAN

NCBI Protein Information

POU domain, class 2, transcription factor 3

NCBI Summary for POU2F3

This gene encodes a member of the POU domain family of transcription factors. POU domain transcription factors bind to a specific octamer DNA motif and regulate cell type-specific differentiation pathways. The encoded protein is primarily expressed in the epidermis, and plays a critical role in keratinocyte proliferation and differentiation. The encoded protein is also a candidate tumor suppressor protein, and aberrant promoter methylation of this gene may play a role in cervical cancer. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Sep 2011]

UniProt Comments for POU2F3

Oct11: Transcription factor that binds to the octamer motif (5'-ATTTGCAT-3'). Regulated the expression of a number of genes such as SPRR2A or placental lactogen. Belongs to the POU transcription factor family. Class- 2 subfamily. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Transcription factor; DNA-binding

Chromosomal Location of Human Ortholog: 11q23.3

Cellular Component: cytoplasm; nucleoplasm; nucleus

Molecular Function: protein binding; sequence-specific DNA binding

Biological Process: epidermis development; positive regulation of transcription from RNA polymerase II promoter; regulation of transcription from RNA polymerase II promoter; transcription from RNA polymerase II promoter

Research Articles on POU2F3

1. Analysis of the transcription factor POU2F3 revealed a DOPr-mediated extracellular signal-regulated kinase (ERK)-dependent downregulation of this factorThe delta-opioid receptor affects epidermal homeostasis

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Pathways associated with anti-POU2F3 antibody

Products by Pathway	Pathway Diagram
Herpes Simplex Infection Pathway antibodies	Herpes Simplex Infection Pathway Diagram
Herpes Simplex Infection Pathway antibodies	Herpes Simplex Infection Pathway Diagram

Diseases associated with anti-POU2F3 antibody

Disease Name	Pubmed Publications
Uterine Cervical Neoplasms Antibodies	>2 publications with POU2F3 and Uterine Cervical Neoplasms
Hyperplasia Antibodies	>1 publications with POU2F3 and Hyperplasia

Organs/Tissues associated with anti-POU2F3 antibody

Organ/Tissue Name	Pubmed Publications
Skin Antibodies	>7 publications with POU2F3 and Skin
Brain Antibodies	>3 publications with POU2F3 and Brain
Intestine Antibodies	>2 publications with POU2F3 and Intestine
Testis Antibodies	>1 publications with POU2F3 and Testis