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POU3F2 Antibody – Goat Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS422363

MBS422363 is an antibody targeting POU3F2. Also known as Goat anti-POU3F2/BRN2/OCT7 Antibody. Reactive in Tested: Human, Mouse, Rat; Expected from sequence similarity: Human, Mouse, Rat, Cow, Pig. Host: Goat. More details

$450 / 0.1 mg +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
POU3F2/BRN2/OCT7, Polyclonal Antibody
Full Product Name
Goat anti-POU3F2/BRN2/OCT7 Antibody
Product Gene Name
anti-POU3F2 antibody
Product Synonym Gene Name
BRN2; OCT7
Product Synonym Names
POU3F2; POU class 3 homeobox 2; BRN2; OCT7; OTF7; POUF3; POU domain, class 3, transcription factor 2; BRN2 antibody; OCT7 antibody; OTF7 antibody; POU class 3 homeobox 2 antibody; POU domain; class 3; transcription factor 2 antibody; POUF3 antibody; POU3F2 antibody; POU3F2 / BRN2 / OCT7
Immunogen Sequence
AQSLVQGDYG ALQSN
Clonality
Polyclonal
Host
Goat
Species Reactivity
Tested: Human, Mouse, Rat; Expected from sequence similarity: Human, Mouse, Rat, Cow, Pig
Form/Format
Supplied at 0.5 mg/ml in Tris saline, 0. 02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Purity/Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Concentration
100ug specific antibody in 200ul (lot specific)
Preparation and Storage
Aliquot and store at -20 degree C. Minimize freezing and thawing.
Antibody/Peptide Pairs
POU3F2 / BRN2 / OCT7 peptide (MBS427295) is used for blocking the activity of POU3F2/BRN2/OCT7 antibody (MBS422363)
Molecular Weight
26,841 Da
OMIM
600494
Immunogen
Peptide with sequence C-AQSLVQGDYGALQSN, from the internal region (near N Terminus) of the protein sequence according to NP_005595.2.
Epitope
Internal region (near N Terminus)
ISO Certification
Manufactured in an ISO 9001:2008 Certified Laboratory.
Applications Tested/Suitable for anti-POU3F2 antibody
Peptide ELISA (EIA), Western Blot (WB)
Application Notes for anti-POU3F2 antibody
Peptide ELISA: Antibody detection limit dilution 1: 8000.
Western Blot: Approx 45kDa band observed in Human Brain (Cerebellum) and in Mouse and Rat Brain lysates (calculated MW of 46.9kDa according to Human NP_005595.2 and 47.1kDa according to Mouse NP_032925.1). Recommended concentration: 0.1-0.3ug/ml.

Western Blot (WB) of anti-POU3F2 antibody

(0.1ug/ml) staining of Mouse Brain lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Goat Anti-POU3F2 Polyclonal Antibody – Cat# MBS422363

Other Notes

Small volumes of anti-POU3F2 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for POU3F2. It may not necessarily be applicable to this product.

NCBI GI #
51702521
NCBI GeneID
5454
NCBI Accession #
NP_005595.2
UniProt Secondary Accession #
Q14960; Q86V54; Q9UJL0
UniProt Related Accession #
P20265
GenBank Nucleotide #
NM_005604.3
NCBI Official Full Name
POU domain, class 3, transcription factor 2
NCBI Official Synonym Full Names
POU class 3 homeobox 2
NCBI Official Symbol
POU3F2
NCBI Official Synonym Symbols
BRN2; OCT7; OTF7; OTF-7; POUF3; brn-2; oct-7; N-Oct3
UniProt Protein Name
POU domain, class 3, transcription factor 2
UniProt Synonym Protein Names
Brain-specific homeobox/POU domain protein 2; Brain-2; Brn-2; Nervous system-specific octamer-binding transcription factor N-Oct-3; Octamer-binding protein 7; Oct-7; Octamer-binding transcription factor 7; OTF-7
Protein Family
POU domain, class 3, transcription factor
UniProt Gene Name
POU3F2
UniProt Synonym Gene Names
BRN2; OCT7; OTF7; Brain-2; Brn-2; Oct-7; OTF-7
UniProt Entry Name
PO3F2_HUMAN
NCBI Protein Information
POU domain, class 3, transcription factor 2

NCBI Summary for POU3F2

This gene encodes a member of the POU-III class of neural transcription factors. The encoded protein is involved in neuronal differentiation and enhances the activation of corticotropin-releasing hormone regulated genes. Overexpression of this protein is associated with an increase in the proliferation of melanoma cells. [provided by RefSeq, Mar 2012]
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UniProt Comments for POU3F2

Oct7: Transcription factor that binds preferentially to the recognition sequence which consists of two distinct half-sites, ('GCAT') and ('TAAT'), separated by a nonconserved spacer region of 0, 2, or 3 nucleotides. Positively regulates the genes under the control of corticotropin-releasing hormone (CRH) and CRH II promoters. Interacts with PQBP1. Expressed specifically in the neuroectodermal cell lineage. Belongs to the POU transcription factor family. Class- 3 subfamily. 3 isoforms of the human protein are produced by alternative initiation.

Protein type: DNA-binding; Transcription factor; Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 6q16

Cellular Component: nucleus; transcription factor complex

Molecular Function: identical protein binding; protein binding; transcription factor activity

Biological Process: astrocyte development; cerebral cortex radially oriented cell migration; epidermis development; forebrain ventricular zone progenitor cell division; hypothalamus cell differentiation; myelination in the peripheral nervous system; neurohypophysis development; neuron differentiation; positive regulation of cell proliferation; positive regulation of multicellular organism growth; positive regulation of transcription from RNA polymerase II promoter; regulation of axonogenesis; transcription from RNA polymerase II promoter
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Research Articles on POU3F2

1. BRN2 is a higher level regulator than ASCL1 and ND1 and BRN2 might be involved in aggressiveness of small cell lung cancer.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Pathways associated with anti-POU3F2 antibody

 Products by Pathway  Pathway Diagram
 SIDS Susceptibility Pathways antibodies  SIDS Susceptibility Pathways Diagram

Diseases associated with anti-POU3F2 antibody

 Disease Name  Pubmed Publications
 Brain Diseases Antibodies  >10 publications with POU3F2 and Brain Diseases
 Neoplasms, Experimental Antibodies  >5 publications with POU3F2 and Neoplasms, Experimental
 Neoplasm Metastasis Antibodies  >4 publications with POU3F2 and Neoplasm Metastasis
 Disease Models, Animal Antibodies  >3 publications with POU3F2 and Disease Models, Animal
 Lung Neoplasms Antibodies  >3 publications with POU3F2 and Lung Neoplasms
 Inflammation Antibodies  >2 publications with POU3F2 and Inflammation
 Necrosis Antibodies  >2 publications with POU3F2 and Necrosis
 Lung Diseases Antibodies  >1 publications with POU3F2 and Lung Diseases
 Kidney Diseases Antibodies  >1 publications with POU3F2 and Kidney Diseases
 Neurobehavioral Manifestations Antibodies  >1 publications with POU3F2 and Neurobehavioral Manifestations

Organs/Tissues associated with anti-POU3F2 antibody

 Organ/Tissue Name  Pubmed Publications
 Brain Antibodies  >29 publications with POU3F2 and Brain
 Embryonic Tissue Antibodies  >20 publications with POU3F2 and Embryonic Tissue
 Skin Antibodies  >6 publications with POU3F2 and Skin
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