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POU3F3 Antibody – Goat Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS422364

MBS422364 is an antibody targeting POU3F3. Also known as Goat anti-POU3F3/BRN1/OCT8 Antibody. Reactive in Tested: Mouse, RatExpected from sequence similarity: Human, Mouse, Rat. Host: Goat. More details

$450 / 0.1 mg +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
POU3F3/BRN1/OCT8, Polyclonal Antibody
Full Product Name
Goat anti-POU3F3/BRN1/OCT8 Antibody
Product Gene Name
anti-POU3F3 antibody
Product Synonym Gene Name
BRN1; OCT8
Product Synonym Names
POU3F3; POU class 3 homeobox 3; BRN1; OTF8; OTF-8; POU domain, class 3, transcription factor 3; brain-1; brain-specific homeobox/POU domain protein 1; brn-1; oct-8; octamer-binding protein 8; octamer-binding transcription factor 8; Brain-1 antibody; BRN1 antibody; OTF8 antibody; POU class 3 homeobox 3 antibody; POU domain; class 3; transcription factor 3 antibody; POU3F3 antibody; OTF-8 antibody; brain-specific homeobox/POU domain protein 1 antibody; brn-1 antibody; oct-8 antibody; octamer-binding protein 8 antibody; octamer-binding transcription factor 8 antibody; POU3F3 / BRN1 / OCT8
Immunogen Sequence
HMLSHAHQWV TAL
Clonality
Polyclonal
Host
Goat
Species Reactivity
Tested: Mouse, Rat
Expected from sequence similarity: Human, Mouse, Rat
Form/Format
Supplied at 0.5 mg/ml in Tris saline, 0. 02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Purity/Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Concentration
100ug specific antibody in 200ul (lot specific)
Preparation and Storage
Aliquot and store at -20°C. Minimize freezing and thawing.
Antibody/Peptide Pairs
POU3F3 / BRN1 / OCT8 peptide (MBS427296) is used for blocking the activity of POU3F3/BRN1/OCT8 antibody (MBS422364)
OMIM
602480
Immunogen
Peptide with sequence C-HMLSHAHQWVTAL, from the internal region of the protein sequence according to NP_006227.1.
ISO Certification
Manufactured in an ISO 9001:2008 Certified Laboratory.
Applications Tested/Suitable for anti-POU3F3 antibody
Peptide ELISA (EIA), Western Blot (WB)
Application Notes for anti-POU3F3 antibody
Peptide ELISA: Antibody detection limit dilution 1: 4000.
Western Blot: Approx 48kDa band observed in Mouse and Rat Spinal Cord lysates (calculated MW of 50.3kDa according to Human NP_006227.1 and 50.2kDa according to Mouse NP_032926.2). Recommended concentration: 1-3ug/ml.

Western Blot (WB) of anti-POU3F3 antibody

(1ug/ml) staining of Mouse Spinal Cord lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Goat Anti-POU3F3 Polyclonal Antibody – Cat# MBS422364

Other Notes

Small volumes of anti-POU3F3 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for POU3F3. It may not necessarily be applicable to this product.

NCBI GI #
5453936
NCBI GeneID
5455
NCBI Accession #
NP_006227.1
UniProt Secondary Accession #
P78379; Q4ZG25
UniProt Related Accession #
P20264
GenBank Nucleotide #
NM_006236.2
NCBI Official Full Name
POU domain, class 3, transcription factor 3
NCBI Official Synonym Full Names
POU class 3 homeobox 3
NCBI Official Symbol
POU3F3
NCBI Official Synonym Symbols
BRN1; OTF8; oct-8; brain-1
UniProt Protein Name
POU domain, class 3, transcription factor 3
UniProt Synonym Protein Names
Brain-specific homeobox/POU domain protein 1; Brain-1; Brn-1; Octamer-binding protein 8; Oct-8; Octamer-binding transcription factor 8; OTF-8
Protein Family
POU domain, class 3, transcription factor
UniProt Gene Name
POU3F3
UniProt Synonym Gene Names
BRN1; OTF8; Brain-1; Brn-1; Oct-8; OTF-8
UniProt Entry Name
PO3F3_HUMAN
NCBI Protein Information
POU domain, class 3, transcription factor 3

NCBI Summary for POU3F3

This gene encodes a POU-domain containing protein that functions as a transcription factor. The encoded protein recognizes an octamer sequence in the DNA of target genes. This protein may play a role in development of the nervous system. [provided by RefSeq, Apr 2015]

UniProt Comments for POU3F3

Oct8: Transcription factor that acts synergistically with SOX11 and SOX4. Plays a role in neuronal development. Is implicated in an enhancer activity at the embryonic met- mesencephalic junction; the enhancer element contains the octamer motif (5'-ATTTGCAT-3'). Belongs to the POU transcription factor family. Class- 3 subfamily.

Protein type: DNA-binding; Transcription factor

Chromosomal Location of Human Ortholog: 2q12.1

Cellular Component: nucleus

Molecular Function: sequence-specific DNA binding; transcription factor activity

Biological Process: central nervous system development; cerebral cortex radially oriented cell migration; forebrain ventricular zone progenitor cell division; negative regulation of apoptosis; negative regulation of transcription, DNA-dependent; positive regulation of cell proliferation; positive regulation of transcription from RNA polymerase II promoter; positive regulation of transcription, DNA-dependent; transcription, DNA-dependent
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Research Articles on POU3F3

1. linc-POU3F3 might affect glioma development via altering expression level of POU3F3, and may also have a crucial regulatory role in glioma progression

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-POU3F3 antibody

 Reference Product  PubMed Publications
 POU3F1 antibody  >4 publications with POU3F3 and POU3F1
 SOX11 antibody  >4 publications with POU3F3 and SOX11
 DLL1 antibody  >1 publications with POU3F3 and DLL1
 ASCL1 antibody  >1 publications with POU3F3 and ASCL1
 BMP4 antibody  >1 publications with POU3F3 and BMP4

Diseases associated with anti-POU3F3 antibody

 Disease Name  Pubmed Publications
 Nervous System Diseases Antibodies  >3 publications with POU3F3 and Nervous System Diseases
 Disease Models, Animal Antibodies  >3 publications with POU3F3 and Disease Models, Animal
 Adenocarcinoma Antibodies  >2 publications with POU3F3 and Adenocarcinoma
 Neoplasms, Experimental Antibodies  >2 publications with POU3F3 and Neoplasms, Experimental
 Kidney Diseases Antibodies  >2 publications with POU3F3 and Kidney Diseases
 Neurobehavioral Manifestations Antibodies  >1 publications with POU3F3 and Neurobehavioral Manifestations
 Inflammation Antibodies  >1 publications with POU3F3 and Inflammation
 Osteoarthritis Antibodies  >1 publications with POU3F3 and Osteoarthritis

Organs/Tissues associated with anti-POU3F3 antibody

 Organ/Tissue Name  Pubmed Publications
 Brain Antibodies  >19 publications with POU3F3 and Brain
 Embryonic Tissue Antibodies  >15 publications with POU3F3 and Embryonic Tissue
 Kidney Antibodies  >8 publications with POU3F3 and Kidney
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