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DCTN3 Antibody – Goat Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS422499

MBS422499 is an antibody targeting DCTN3. Also known as Goat anti-DCTN3 Antibody. Reactive in Tested: Human, Mouse, RatExpected from sequence similarity: Human, Mouse, Rat, Dog, Cow. Host: Goat. More details

$450 / 0.1 mg +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
DCTN3, Polyclonal Antibody
Full Product Name
Goat anti-DCTN3 Antibody
Product Gene Name
anti-DCTN3 antibody
Product Synonym Names
DCTN3; dynactin 3 (p22); DCTN-22; DCTN22; MGC111190; dynactin 3; dynactin 3, isoform 1; dynactin light chain; DCTN22 antibody; DCTN-22 antibody; dynactin 3 antibody; dynactin 3 (p22) antibody; dynactin 3; isoform 1 antibody; dynactin light chain antibody; MGC111190 antibody; DCTN3 antibody
Clonality
Polyclonal
Host
Goat
Species Reactivity
Tested: Human, Mouse, Rat
Expected from sequence similarity: Human, Mouse, Rat, Dog, Cow
Form/Format
Supplied at 0.5 mg/ml in Tris saline, 0. 02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Purity/Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Concentration
100ug specific antibody in 200ul (lot specific)
Preparation and Storage
Aliquot and store at -20 degree C. Minimize freezing and thawing.
Antibody/Peptide Pairs
DCTN3 peptide (MBS427704) is used for blocking the activity of DCTN3 antibody (MBS422499)
OMIM
607387
Immunogen
Peptide with sequence C-QVALGNISSKRE, from the internal region of the protein sequence according to NP_009165.1; NP_077324.1.
Note
This antibody is expected to recognize both reported isoforms (NP_009165.1; NP_077324.1).
ISO Certification
Manufactured in an ISO 9001:2008 Certified Laboratory.
Applications Tested/Suitable for anti-DCTN3 antibody
Peptide ELISA (EIA), Western Blot (WB)
Application Notes for anti-DCTN3 antibody
Peptide ELISA: Antibody detection limit dilution 1: 16000.
Western Blot: Approx 20kDa band observed in Human Kidney, Skeletal Muscle and Pancreas lysates (calculated MW of 19.5kDa according to NP_077324.1). In addition, a 25kDa band is observed in both Mouse and Rat Skeletal Muscle lysates accompanied by an 18kDa band in Mouse Skeletal Msuscle. In Mouse the protein is known as p24. The 18kDa band could reprsent a smaller isoform. Recommended concentration: 0. 03-0.1ug/ml.

Western Blot (WB) of anti-DCTN3 antibody

MBS422499 (0. 03ug/ml) staining of Human Kidney lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Goat Anti-DCTN3 Polyclonal Antibody – Cat# MBS422499

Western Blot (WB) of anti-DCTN3 antibody

MBS422499 (0.2ug/ml) staining of Mouse (A) and Rat (B) Skeletal Muscle lysates (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Goat Anti-DCTN3 Polyclonal Antibody, Western Blot (WB) – Cat# MBS422499

Other Notes

Small volumes of anti-DCTN3 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for DCTN3. It may not necessarily be applicable to this product.

NCBI GI #
6005745
NCBI GeneID
11258
NCBI Accession #
NP_009165.1
NCBI Related Accession #
NP_077324.1
UniProt Secondary Accession #
Q5T1I5; Q5T1I7; Q5T8G3; Q9BPU8; A6NII7; B2RBM5
UniProt Related Accession #
O75935
GenBank Nucleotide #
NM_007234.4
NCBI Official Full Name
dynactin subunit 3 isoform 1
NCBI Official Synonym Full Names
dynactin subunit 3
NCBI Official Symbol
DCTN3
NCBI Official Synonym Symbols
DCTN22; DCTN-22
UniProt Protein Name
Dynactin subunit 3
UniProt Synonym Protein Names
Dynactin complex subunit 22 kDa subunit; p22
Protein Family
Dynactin
UniProt Gene Name
DCTN3
UniProt Synonym Gene Names
p22
UniProt Entry Name
DCTN3_HUMAN
NCBI Protein Information
dynactin subunit 3

NCBI Summary for DCTN3

This gene encodes the smallest subunit of dynactin, a macromolecular complex consisting of 10 subunits ranging in size from 22 to 150 kD. Dynactin binds to both microtubules and cytoplasmic dynein. It is involved in a diverse array of cellular functions, including ER-to-Golgi transport, the centripetal movement of lysosomes and endosomes, spindle formation, cytokinesis, chromosome movement, nuclear positioning, and axonogenesis. This subunit, like most other dynactin subunits, exists only as a part of the dynactin complex. It is primarily an alpha-helical protein with very little coiled coil, and binds directly to the largest subunit (p150) of dynactin. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2013]
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UniProt Comments for DCTN3

dynactin 3: Together with dynein may be involved in spindle assembly and cytokinesis. Belongs to the dynactin subunit 3 family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Microtubule-binding; Motility/polarity/chemotaxis; Motor

Chromosomal Location of Human Ortholog: 9p13

Cellular Component: centrosome; cleavage furrow; cytosol; dynactin complex; midbody; perinuclear region of cytoplasm; spindle

Molecular Function: protein binding; structural molecule activity

Biological Process: antigen processing and presentation of exogenous peptide antigen via MHC class II; cytokinesis; ER to Golgi vesicle-mediated transport; G2/M transition of mitotic cell cycle; microtubule-based process; mitosis
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Pathways associated with anti-DCTN3 antibody

 Products by Pathway  Pathway Diagram
 AURKA Activation By TPX2 Pathway antibodies  AURKA Activation By TPX2 Pathway Diagram
 Adaptive Immune System Pathway antibodies  Adaptive Immune System Pathway Diagram
 Anchoring Of The Basal Body To The Plasma Membrane Pathway antibodies  Anchoring Of The Basal Body To The Plasma Membrane Pathway Diagram
 Asparagine N-linked Glycosylation Pathway antibodies  Asparagine N-linked Glycosylation Pathway Diagram
 Assembly Of The Primary Cilium Pathway antibodies  Assembly Of The Primary Cilium Pathway Diagram
 COPI-independent Golgi-to-ER Retrograde Traffic Pathway antibodies  COPI-independent Golgi-to-ER Retrograde Traffic Pathway Diagram
 COPI-mediated Anterograde Transport Pathway antibodies  COPI-mediated Anterograde Transport Pathway Diagram
 Cell Cycle Pathway antibodies  Cell Cycle Pathway Diagram
 Cell Cycle, Mitotic Pathway antibodies  Cell Cycle, Mitotic Pathway Diagram
 Centrosome Maturation Pathway antibodies  Centrosome Maturation Pathway Diagram

Diseases associated with anti-DCTN3 antibody

 Disease Name  Pubmed Publications
 Atrophy Antibodies  >1 publications with DCTN3 and Atrophy
 Chromosome Aberrations Antibodies  >1 publications with DCTN3 and Chromosome Aberrations
 Precancerous Conditions Antibodies  >1 publications with DCTN3 and Precancerous Conditions
 Neoplasm Metastasis Antibodies  >1 publications with DCTN3 and Neoplasm Metastasis
 Prostatic Diseases Antibodies  >1 publications with DCTN3 and Prostatic Diseases

Organs/Tissues associated with anti-DCTN3 antibody

 Organ/Tissue Name  Pubmed Publications
 Mouth Antibodies  >2 publications with DCTN3 and Mouth
 Lymph Antibodies  >1 publications with DCTN3 and Lymph
 Lymph Node Antibodies  >1 publications with DCTN3 and Lymph Node
 Blood Antibodies  >1 publications with DCTN3 and Blood
 Prostate Antibodies  >1 publications with DCTN3 and Prostate
 Brain Antibodies  >1 publications with DCTN3 and Brain
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