Goat Anti-TH1L Polyclonal Antibody – Cat# MBS422501

(0.5ug/ml) staining of nuclear Jurkat lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

TH1L Antibody – Goat Polyclonal

Name: TH1L Antibody; Polyclonal; Goat; Human-Reactive; MyBioSource
Brand: MyBioSource
SKU: MBS422501
Price: 450 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS422501

MBS422501 is an antibody targeting TH1L. Also known as Goat anti-TH1L Antibody. Reactive in Tested: Human; Expected from sequence similarity: Human, Mouse, Dog, Cow, Pig. Host: Goat. More details

$450 / 0.1 mg +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Online Datasheet in PDF

Product Name

TH1L, Polyclonal Antibody

Full Product Name

Goat anti-TH1L Antibody

Product Gene Name

anti-TH1L antibody

Product Synonym Names

TH1L; TH1-like (Drosophila); HSPC130; NELF-C; NELF-D; TH1; TH1 drosophila homolog; TH1-like protein; negative elongation factor proteins C and D; trihydrophobin 1; HSPC130 antibody; negative elongation factor proteins C and D antibody; NELF-C antibody; NELF-D antibody; TH1 antibody; TH1 drosophila homolog antibody; TH1-like (Drosophila) antibody; TH1-like protein antibody; trihydrophobin 1 antibody; TH1L antibody

Immunogen Sequence

NKRVSINKDE

Clonality

Polyclonal

Host

Goat

Species Reactivity

Tested: Human; Expected from sequence similarity: Human, Mouse, Dog, Cow, Pig

Form/Format

Supplied at 0.5 mg/ml in Tris saline, 0. 02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

Purity/Purification

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Concentration

100ug specific antibody in 200ul (lot specific)

Preparation and Storage

Aliquot and store at -20 degree C. Minimize freezing and thawing.

Antibody/Peptide Pairs

TH1L peptide (MBS427684) is used for blocking the activity of TH1L antibody (MBS422501)

Molecular Weight

65,479 Da

OMIM

605297

Immunogen

Peptide with sequence C-NKRVSINKDE, from the internal region of the protein sequence according to NP_945327.1.

Epitope

Internal region

ISO Certification

Manufactured in an ISO 9001:2008 Certified Laboratory.

Applications Tested/Suitable for anti-TH1L antibody

Peptide ELISA (EIA), Western Blot (WB)

Application Notes for anti-TH1L antibody

Peptide ELISA: Antibody detection limit dilution 1: 16000.
Western Blot: Approx 60kDa band observed in nuclear lysates of cell line Jurkat (calculated MW of 66.2kDa according to NP_945327.1). Recommended concentration: 0.5-2ug/ml.

Other Notes

Small volumes of anti-TH1L antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for TH1L. It may not necessarily be applicable to this product.

NCBI GI #

39812492

NCBI GeneID

51497

NCBI Accession #

NP_945327.1

UniProt Secondary Accession #

Q9BYL2; Q9H405; Q9H888; Q9H8T3; Q9NVX5; Q9P029; Q9UGN1; Q9UGN2; Q9UGN3; B4DE06

UniProt Related Accession #

Q8IXH7

GenBank Nucleotide #

NM_198976.1

NCBI Official Full Name

negative elongation factor C/D

NCBI Official Synonym Full Names

negative elongation factor complex member C/D

NCBI Official Symbol

NELFCD

NCBI Official Synonym Symbols

TH1; TH1L; NELF-C; NELF-D; HSPC130

UniProt Protein Name

Negative elongation factor C/D

UniProt Synonym Protein Names

TH1-like protein

UniProt Gene Name

NELFCD

UniProt Synonym Gene Names

NELFD; TH1; TH1L; NELF-C/D

UniProt Entry Name

NELFD_HUMAN

NCBI Protein Information

negative elongation factor C/D

NCBI Summary for TH1L

The NELF complex of proteins interacts with the DSIF protein complex to repress transcriptional elongation by RNA polymerase II. The protein encoded by this gene is an essential part of the NELF complex. Alternative translation initiation site usage results in the formation of two isoforms with different N-termini. [provided by RefSeq, Jul 2008]

UniProt Comments for TH1L

NELFCD: Essential component of the NELF complex, a complex that negatively regulates the elongation of transcription by RNA polymerase II. The NELF complex, which acts via an association with the DSIF complex and causes transcriptional pausing, is counteracted by the P-TEFb kinase complex. The NELF complex is composed of 4 subunits: WHSC2/NELF-A, COBRA1/NELF-B, TH1L (isoform NELF-C or isoform NELF-D) and RDBP/NELF-E. Interacts with ARAF1. Widely expressed. Expressed in heart, brain, lung, placenta, liver, skeletal and cardiac muscle, adrenal, thyroid, kidney and pancreas. Belongs to the NELF-D family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Adaptor/scaffold

Chromosomal Location of Human Ortholog: 20q13

Cellular Component: membrane; nucleoplasm

Molecular Function: protein binding

Biological Process: negative regulation of transcription, DNA-dependent; positive regulation of viral transcription; RNA elongation from RNA polymerase II promoter; transcription from RNA polymerase II promoter

Research Articles on TH1L

1. data show that NELF subunits exhibit highly specific subcellular localizations, such as in NELF bodies or in midbodies, and some shuttle actively between the nucleus and cytoplasm; loss of NELF from cells can lead to enlarged and/or multiple nuclei

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Pathways associated with anti-TH1L antibody

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Abortive Elongation Of HIV-1 Transcript In The Absence Of Tat Pathway antibodies	Abortive Elongation Of HIV-1 Transcript In The Absence Of Tat Pathway Diagram
Disease Pathway antibodies	Disease Pathway Diagram
Formation Of HIV Elongation Complex In The Absence Of HIV Tat Pathway antibodies	Formation Of HIV Elongation Complex In The Absence Of HIV Tat Pathway Diagram
Formation Of HIV-1 Elongation Complex Containing HIV-1 Tat Pathway antibodies	Formation Of HIV-1 Elongation Complex Containing HIV-1 Tat Pathway Diagram
Formation Of RNA Pol II Elongation Complex Pathway antibodies	Formation Of RNA Pol II Elongation Complex Pathway Diagram
Formation Of The Early Elongation Complex Pathway antibodies	Formation Of The Early Elongation Complex Pathway Diagram
Formation Of The HIV-1 Early Elongation Complex Pathway antibodies	Formation Of The HIV-1 Early Elongation Complex Pathway Diagram
Gene Expression Pathway antibodies	Gene Expression Pathway Diagram
Generic Transcription Pathway antibodies	Generic Transcription Pathway Diagram
HIV Infection Pathway antibodies	HIV Infection Pathway Diagram