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NEK7 Antibody – Goat Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS422522

MBS422522 is an antibody targeting NEK7. Also known as Goat anti-NEK7 Antibody. Reactive in Tested: Human; Expected from sequence similarity: Human, Mouse, Rat, Dog, Cow. Host: Goat. More details

$450 / 0.1 mg +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
NEK7, Polyclonal Antibody
Full Product Name
Goat anti-NEK7 Antibody
Product Gene Name
Product Synonym Names
Goat Anti-NEK7 (aa 274-286) Antibody; NEK7; NIMA (never in mitosis gene a)-related kinase 7; NIMA-related kinase 7; NIMA (never in mitosis gene a)-related kinase 7 antibody; NIMA-related kinase 7 antibody; NEK7 antibody
Immunogen Sequence
NTLANFRIEK K
Clonality
Polyclonal
Host
Goat
Species Reactivity
Tested: Human; Expected from sequence similarity: Human, Mouse, Rat, Dog, Cow
Form/Format
Supplied at 0.5 mg/ml in Tris saline, 0. 02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Purity/Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Concentration
100ug specific antibody in 200ul (lot specific)
Preparation and Storage
Aliquot and store at -20 degree C. Minimize freezing and thawing.
Antibody/Peptide Pairs
NEK7 peptide (MBS428173) is used for blocking the activity of NEK7 antibody (MBS422522)
Molecular Weight
13,402 Da
OMIM
606848
Immunogen
Peptide with sequence C-NTLANFRIEKK, from the internal region of the protein sequence according to NP_598001.1.
Epitope
Internal region
ISO Certification
Manufactured in an ISO 9001:2008 Certified Laboratory.
Applications Tested/Suitable for anti-NEK7 antibody
Peptide ELISA (EIA), Western Blot (WB)
Application Notes for anti-NEK7 antibody
Peptide ELISA: Antibody detection limit dilution 1: 64000.
Western Blot: Approx 35kDa band observed in lysates of cell line HeLa (calculated MW of 34.6kDa according to NP_598001.1). Recommended concentration: 0. 05-0.2ug/ml.

Western Blot (WB) of anti-NEK7 antibody

(0. 05ug/ml) staining of HeLa lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Goat Anti-NEK7 Polyclonal Antibody – Cat# MBS422522

Other Notes

Small volumes of anti-NEK7 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for NEK7. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Secondary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
serine/threonine-protein kinase Nek7
NCBI Official Synonym Full Names
NIMA related kinase 7
NCBI Official Symbol
NEK7
UniProt Protein Name
Serine/threonine-protein kinase Nek7
UniProt Synonym Protein Names
Never in mitosis A-related kinase 7; NimA-related protein kinase 7
UniProt Gene Name
NEK7
UniProt Synonym Gene Names
NimA-related protein kinase 7
UniProt Entry Name
NEK7_HUMAN
NCBI Protein Information
serine/threonine-protein kinase Nek7

NCBI Summary for NEK7

NIMA-related kinases share high amino acid sequence identity with the gene product of the Aspergillus nidulans 'never in mitosis A' gene, which controls initiation of mitosis.[supplied by OMIM, Jul 2002]

UniProt Comments for NEK7

NEK7: a protein kinase of the NEK family. Phosphorylates and activates p70S6 kinase.

Protein type: Kinase, protein; EC 2.7.11.1; Protein kinase, Other; Protein kinase, Ser/Thr (non-receptor); Other group; NEK family

Chromosomal Location of Human Ortholog: 1q31.3

Cellular Component: cytoplasm; microtubule; microtubule organizing center; nucleoplasm; spindle pole

Molecular Function: ATP binding; metal ion binding; protein serine/threonine kinase activity

Biological Process: cytokinesis; positive regulation of telomerase activity; positive regulation of telomere maintenance via telomerase; protein amino acid phosphorylation; regulation of mitotic cell cycle; spindle assembly
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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