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LIMS2 Antibody – Goat Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS422526

MBS422526 is an antibody targeting LIMS2. Also known as Goat anti-LIMS2 Antibody. Reactive in Expected from sequence similarity: Human. Host: Goat. More details

$450 / 0.1 mg +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Product Overview

Online Datasheet in PDF
Product Name
LIMS2, Polyclonal Antibody
Full Product Name
Goat anti-LIMS2 Antibody
Product Gene Name
Product Synonym Names
LIMS2; LIM and senescent cell antigen-like domains 2; FLJ10044; PINCH2; ILK-binding protein; OTTHUMP00000203848; OTTHUMP00000203850; OTTHUMP00000203866; FLJ10044 antibody; ILK-binding protein antibody; LIM and senescent cell antigen-like domains 2 antibody; OTTHUMP00000203848 antibody; OTTHUMP00000203850 antibody; OTTHUMP00000203866 antibody; PINCH2 antibody; LIMS2 antibody
Immunogen Sequence
RWSNMSDALA
Clonality
Polyclonal
Host
Goat
Species Reactivity
Expected from sequence similarity: Human
Form/Format
Supplied at 0.5 mg/ml in Tris saline, 0. 02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Purity/Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Concentration
100ug specific antibody in 200ul (lot specific)
Preparation and Storage
Aliquot and store at -20 degree C. Minimize freezing and thawing.
Antibody/Peptide Pairs
LIMS2 peptide (MBS427546) is used for blocking the activity of LIMS2 antibody (MBS422526)
Molecular Weight
41,226 Da
OMIM
607908
Immunogen
Peptide with sequence C-RWSNMSDALA, from the internal region of the protein sequence according to NP_001129509.2; NP_001154875.1.
Epitope
Internal region
Note
This antibody is expected to recognize isoform 1 (NP_001129509.2) and isoformn 3 (NP_001154875.1).
ISO Certification
Manufactured in an ISO 9001:2008 Certified Laboratory.
Applications Tested/Suitable for anti-LIMS2 antibody
Peptide ELISA (EIA)
Application Notes for anti-LIMS2 antibody
Peptide ELISA: Antibody detection limit dilution 1: 8000.
Western Blot: Preliminary experiments gave an approx 80kDa band in Human Heart and Mouse Heart lysates after 0.5 ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 41.2kDa according to NP_001129509.2. The 80kDa band was successfully blocked by incubation with the immunizing peptide.

Other Notes

Small volumes of anti-LIMS2 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for LIMS2. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI Related Accession #
UniProt Secondary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
LIM and senescent cell antigen-like-containing domain protein 2 isoform 1
NCBI Official Synonym Full Names
LIM zinc finger domain containing 2
NCBI Official Symbol
LIMS2
NCBI Official Synonym Symbols
LGMD2W; PINCH2; PINCH-2
UniProt Protein Name
LIM and senescent cell antigen-like-containing domain protein 2
UniProt Synonym Protein Names
LIM-like protein 2; Particularly interesting new Cys-His protein 2; PINCH-2
UniProt Gene Name
LIMS2
UniProt Synonym Gene Names
PINCH2; PINCH-2
UniProt Entry Name
LIMS2_HUMAN
NCBI Protein Information
LIM and senescent cell antigen-like-containing domain protein 2

NCBI Summary for LIMS2

This gene encodes a member of a small family of focal adhesion proteins which interacts with ILK (integrin-linked kinase), a protein which effects protein-protein interactions with the extraceullar matrix. The encoded protein has five LIM domains, each domain forming two zinc fingers, which permit interactions which regulate cell shape and migration. A pseudogene of this gene is located on chromosome 4. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Nov 2011]
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UniProt Comments for LIMS2

LIMS2: Competes with LIMS1 for binding to ILK. Plays a role in modulating cell spreading and migration. 3 isoforms of the human protein are produced by alternative splicing.

Chromosomal Location of Human Ortholog: 2q14.3

Cellular Component: cytosol; focal adhesion; nucleus; plasma membrane

Molecular Function: protein binding; zinc ion binding

Biological Process: cell-cell adhesion; intercellular junction assembly and maintenance; negative regulation of apoptosis
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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