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MGAT1 Antibody – Goat Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS422530

MBS422530 is an antibody targeting MGAT1. Also known as Goat anti-MGAT1 Antibody. Reactive in Tested: HumanExpected from sequence similarity: Human, Mouse, Rat, Dog, Cow. Host: Goat. More details

$450 / 0.1 mg +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
MGAT1, Polyclonal Antibody
Full Product Name
Goat anti-MGAT1 Antibody
Product Gene Name
Product Synonym Names
MGAT1; mannosyl (alpha-1,3-)-glycoprotein beta-1,2-N-acetylglucosaminyltransferase; GLCNAC-TI; GLCT1; GLYT1; GNT-1; GNT-I; MGAT; N-glycosyl-oligosaccharide-glycoprotein N-acetylglucosaminyltransferase; OTTHUMP00000161546; GLCNAC-TI antibody; GLCT1 antibody; GLYT1 antibody; GNT-1 antibody; GNT-I antibody; mannosyl (alpha-1; 3-)-glycoprotein beta-1; 2-N-acetylglucosaminyltransferase antibody; MGAT antibody; N-glycosyl-oligosaccharide-glycoprotein N-acetylglucosaminyltransferase antibody; OTTHUMP00000161546 antibody; MGAT1 antibody
Clonality
Polyclonal
Host
Goat
Species Reactivity
Tested: Human
Expected from sequence similarity: Human, Mouse, Rat, Dog, Cow
Form/Format
Supplied at 0.5 mg/ml in Tris saline, 0. 02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Purity/Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Concentration
100ug specific antibody in 200ul (lot specific)
Preparation and Storage
Aliquot and store at -20 degree C. Minimize freezing and thawing.
Antibody/Peptide Pairs
MGAT1 peptide (MBS427465) is used for blocking the activity of MGAT1 antibody (MBS422530)
OMIM
160995
Immunogen
Peptide with sequence C-QVEKVRTNDR, from the internal region of the protein sequence according to NP_002397.2.
Epitope
Internal region
Note
Reported variants represent identical protein: NP_002397.2, NP_001108090.1, NP_001108091.1, NP_001108092.1, NP_001108089.1.
ISO Certification
Manufactured in an ISO 9001:2008 Certified Laboratory.
Applications Tested/Suitable for anti-MGAT1 antibody
Peptide ELISA (EIA), Western Blot (WB-Trf)
Application Notes for anti-MGAT1 antibody
Peptide ELISA: Antibody detection limit dilution 1: 8000.
Western Blot: In transfected HEK293 transiently expressing full-length Human MGAT1 (myc and DYKDDDDK tagged), a band of approx.55kDa was observed. No bands were observed in mock-transfected HEK293 and the same band was observed using anti-myc tag antibody. Recommended concentration, 0.2-1ug/ml.

Western Blot (WB) of anti-MGAT1 antibody

HEK293 lysate (10ug protein in RIPA buffer) over expressing Human MGAT1 with C-terminal MYC tag probed with MBS422530 (1ug/ml) in Lane A and probed with anti-MYC Tag (1/1000) in lane C. Mock-transfected HEK293 probed with (1mg/ml) in Lane B. Primary incubations were for 1 hour. Detected by chemiluminescence.
Goat Anti-MGAT1 Polyclonal Antibody – Cat# MBS422530

Other Notes

Small volumes of anti-MGAT1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for MGAT1. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Secondary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
alpha-1,3-mannosyl-glycoprotein 2-beta-N-acetylglucosaminyltransferase
NCBI Official Synonym Full Names
mannosyl (alpha-1,3-)-glycoprotein beta-1,2-N-acetylglucosaminyltransferase
NCBI Official Symbol
MGAT1
NCBI Official Synonym Symbols
GnTI; MGAT; GLCT1; GLYT1; GNT-1; GNT-I; GLCNAC-TI
UniProt Protein Name
Alpha-1,3-mannosyl-glycoprotein 2-beta-N-acetylglucosaminyltransferase
UniProt Synonym Protein Names
N-glycosyl-oligosaccharide-glycoprotein N-acetylglucosaminyltransferase I; GNT-I; GlcNAc-T I
UniProt Gene Name
MGAT1
UniProt Synonym Gene Names
GGNT1; GLCT1; GLYT1; MGAT; GNT-I; GlcNAc-T I
UniProt Entry Name
MGAT1_HUMAN
NCBI Protein Information
alpha-1,3-mannosyl-glycoprotein 2-beta-N-acetylglucosaminyltransferase

NCBI Summary for MGAT1

There are believed to be over 100 different glycosyltransferases involved in the synthesis of protein-bound and lipid-bound oligosaccharides. UDP-N-acetylglucosamine:alpha-3-D-mannoside beta-1,2-N-acetylglucosaminyltransferase I is a medial-Golgi enzyme essential for the synthesis of hybrid and complex N-glycans. The protein, encoded by a single exon, shows typical features of a type II transmembrane protein. The protein is believed to be essential for normal embryogenesis. Several variants encoding the same protein have been found for this gene. [provided by RefSeq, Jul 2008]
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UniProt Comments for MGAT1

MGAT1: Initiates complex N-linked carbohydrate formation. Essential for the conversion of high-mannose to hybrid and complex N-glycans. Belongs to the glycosyltransferase 13 family.

Protein type: EC 2.4.1.101; Glycan Metabolism - N-glycan biosynthesis; Membrane protein, integral; Transferase

Chromosomal Location of Human Ortholog: 5q35

Cellular Component: Golgi membrane; integral to membrane; membrane

Molecular Function: acetylglucosaminyltransferase activity; alpha-1,3-mannosylglycoprotein 2-beta-N-acetylglucosaminyltransferase activity; metal ion binding

Biological Process: carbohydrate metabolic process; in utero embryonic development; protein amino acid glycosylation; UDP-N-acetylglucosamine catabolic process
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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