Goat Anti-RUFY1 Polyclonal Antibody – Cat# MBS422549

(0. 05ug/ml) staining of HeLa lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

RUFY1 Antibody – Goat Polyclonal

Name: RUFY1 Antibody; Polyclonal; Goat; Human-Reactive; MyBioSource
Brand: MyBioSource
SKU: MBS422549
Price: 450 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS422549

MBS422549 is an antibody targeting RUFY1. Also known as Goat anti-RUFY1 Antibody. Reactive in Tested: Human; Expected from sequence similarity: Human, Mouse, Rat, Dog, Cow. Host: Goat. More details

$450 / 0.1 mg +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Online Datasheet in PDF

Product Name

RUFY1, Polyclonal Antibody

Full Product Name

Goat anti-RUFY1 Antibody

Product Gene Name

anti-RUFY1 antibody

Product Synonym Names

RUFY1; RUN and FYVE domain containing 1; FLJ22251; RABIP4; ZFYVE12; FYVE-finger protein EIP1; OTTHUMP00000223250; RUN and FYVE domain-containing 1; RUN and FYVE domain-containing protein 1; la-binding protein 1; rab4-interacting protein; zinc finger FYVE; RUFY1 antibody; RUN and FYVE domain containing 1 antibody; FLJ22251 antibody; RABIP4 antibody; ZFYVE12 antibody; FYVE-finger protein EIP1 antibody; OTTHUMP00000223250 antibody; RUN and FYVE domain-containing 1 antibody; RUN and FYVE domain-containing protein 1 antibody; rab4-interacting protein antibody; zinc finger FYVE antibody

Immunogen Sequence

QKNEAITSFE GKT

Clonality

Polyclonal

Host

Goat

Species Reactivity

Tested: Human; Expected from sequence similarity: Human, Mouse, Rat, Dog, Cow

Form/Format

Supplied at 0.5 mg/ml in Tris saline, 0. 02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

Purity/Purification

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Concentration

100ug specific antibody in 200ul (lot specific)

Preparation and Storage

Aliquot and store at -20 degree C. Minimize freezing and thawing.

Antibody/Peptide Pairs

RUFY1 peptide (MBS427446) is used for blocking the activity of RUFY1 antibody (MBS422549)

Molecular Weight

44,489 Da

OMIM

610327

Immunogen

Peptide with sequence C-QKNEAITSFEGKT, from the internal region of the protein sequence according to NP_079434.3; NP_001035541.1.

Epitope

Internal region

Note

This antibody is expected to recognize both reported isoforms (NP_079434.3; NP_001035541.1). Reported variants represent identical protein: NP_001035542.1, NP_001035541.1.

ISO Certification

Manufactured in an ISO 9001:2008 Certified Laboratory.

Applications Tested/Suitable for anti-RUFY1 antibody

Peptide ELISA (EIA), Western Blot (WB)

Application Notes for anti-RUFY1 antibody

Peptide ELISA: Antibody detection limit dilution 1: 32000.
Western Blot: Approx 85kDa band observed in lysates of cell line HeLa (calculated MW of 79.8kDa according to NP_079434.3). Recommended concentration: 0. 05-0.2ug/ml.

Other Notes

Small volumes of anti-RUFY1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for RUFY1. It may not necessarily be applicable to this product.

NCBI GI #

94721336

NCBI GeneID

80230

NCBI Accession #

NP_079434.3

NCBI Related Accession #

NP_001035541.1

UniProt Secondary Accession #

Q59FF3; Q71S93; Q9H6I3

UniProt Related Accession #

Q96T51

GenBank Nucleotide #

NM_025158.4

NCBI Official Full Name

RUN and FYVE domain-containing protein 1 isoform a

NCBI Official Synonym Full Names

RUN and FYVE domain containing 1

NCBI Official Symbol

RUFY1

NCBI Official Synonym Symbols

RABIP4; ZFYVE12

UniProt Protein Name

RUN and FYVE domain-containing protein 1

UniProt Synonym Protein Names

FYVE-finger protein EIP1; La-binding protein 1; Rab4-interacting protein; Zinc finger FYVE domain-containing protein 12

Protein Family

RUN and FYVE domain-containing protein

UniProt Gene Name

RUFY1

UniProt Synonym Gene Names

RABIP4; ZFYVE12

UniProt Entry Name

RUFY1_HUMAN

NCBI Protein Information

RUN and FYVE domain-containing protein 1

NCBI Summary for RUFY1

This gene encodes a protein that contains a RUN domain and a FYVE-type zinc finger domain. The encoded protein binds to phosphatidylinositol-3-phosphate (PI3P) and plays a role in early endosomal trafficking, tethering and fusion through interactions with small GTPases including Rab4, Rab5 and Rab14. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Dec 2011]

UniProt Comments for RUFY1

RUFY1: Binds phospholipid vesicles containing phosphatidylinositol 3-phosphate and participates in early endosomal trafficking. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Ubiquitin conjugating system

Chromosomal Location of Human Ortholog: 5q35.3

Cellular Component: cytoplasm; early endosome membrane; intracellular membrane-bound organelle; nucleus

Molecular Function: lipid binding; protein binding; protein transporter activity; zinc ion binding

Biological Process: endocytosis; protein transport; regulation of endocytosis

Research Articles on RUFY1

1. Data indicate that RUN and FYVE domain protein Rabip4'(RUFY1) interacts specifically and directly with adaptor protein complex AP-3.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Pathways associated with anti-RUFY1 antibody

Products by Pathway	Pathway Diagram
Endocytosis Pathway antibodies	Endocytosis Pathway Diagram
Endocytosis Pathway antibodies	Endocytosis Pathway Diagram

Organs/Tissues associated with anti-RUFY1 antibody

Organ/Tissue Name	Pubmed Publications
Ovary Antibodies	>1 publications with RUFY1 and Ovary