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Endophilin-A2 (SH3GL1) Antibody – Goat Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS422553

MBS422553 is an antibody targeting SH3GL1. Also known as Goat anti-Endophilin-A2 Antibody. Reactive in Tested: Human; Expected from sequence similarity: Human, Dog, Cow. Host: Goat. More details

$450 / 0.1 mg +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
Endophilin-A2 (SH3GL1), Polyclonal Antibody
Full Product Name
Goat anti-Endophilin-A2 Antibody
Product Gene Name
Product Synonym Names
SH3GL1; SH3-domain GRB2-like 1; CNSA1; EEN; MGC111371; SH3D2B; SH3P8; EEN fusion partner of MLL; SH3 domain GRB2-like 1; SH3 domain protein 2B; SH3 domain-containing GRB2-like protein 1; SH3-containing Grb-2-like 1 protein; SH3-containing protein EEN; end; SH3GL1 antibody; SH3-domain GRB2-like 1 antibody; CNSA1 antibody; EEN antibody; MGC111371 antibody; SH3D2B antibody; SH3P8 antibody; EEN fusion partner of MLL antibody; SH3 domain GRB2-like 1 antibody; SH3 domain-containing GRB2-like protein 1 antibody; SH3-containing Grb-2-like 1 protein antibody; SH3-containing protein EEN antibody; endophilin-A2 antibody; endophilin-2 antibody; SH3 domain protein 2B antibody; extra 11-19 leukemia fusion antibody; extra eleven-nineteen leukemia fusion gene protein antibody; Endophilin-A2; Endophilin-A2
Immunogen Sequence
EPFDLGEPEQ SN
Clonality
Polyclonal
Host
Goat
Species Reactivity
Tested: Human; Expected from sequence similarity: Human, Dog, Cow
Form/Format
Supplied at 0.5 mg/ml in Tris saline, 0. 02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Purity/Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Concentration
100ug specific antibody in 200ul (lot specific)
Preparation and Storage
Aliquot and store at -20 degree C. Minimize freezing and thawing.
Antibody/Peptide Pairs
Molecular Weight
34,519 Da
OMIM
601768
Immunogen
Peptide with sequence C-EPFDLGEPEQSN, from the internal region of the protein sequence according to NP_003016.1.
Epitope
Internal region
ISO Certification
Manufactured in an ISO 9001:2008 Certified Laboratory.
Applications Tested/Suitable for anti-SH3GL1 antibody
Peptide ELISA (EIA), Western Blot (WB)
Application Notes for anti-SH3GL1 antibody
Peptide ELISA: Antibody detection limit dilution 1: 32000.
Western Blot: Approx 48kDa band observed in lysates of cell line K562 (calculated MW of 41.5kDa according to NP_003016.1. The observed molecular weight corresponds to earlier findings in literature with different antibodies (So et al, Proc Natl Acad Sci U S A.1997 Mar 18;94(6): 2563-8.; PMID: 9122235). Recommended concentration: 0.3-1ug/ml.

Western Blot (WB) of anti-SH3GL1 antibody

(0.3ug/ml) staining of K562 lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Goat Anti-SH3GL1 Polyclonal Antibody – Cat# MBS422553

Other Notes

Small volumes of anti-SH3GL1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for SH3GL1. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Secondary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
endophilin-A2 isoform 1
NCBI Official Synonym Full Names
SH3 domain containing GRB2 like 1, endophilin A2
NCBI Official Symbol
SH3GL1
NCBI Official Synonym Symbols
EEN; CNSA1; SH3P8; SH3D2B
UniProt Protein Name
Endophilin-A2
UniProt Synonym Protein Names
EEN fusion partner of MLL; Endophilin-2; Extra eleven-nineteen leukemia fusion gene protein; EEN; SH3 domain protein 2B; SH3 domain-containing GRB2-like protein 1
Protein Family
UniProt Gene Name
SH3GL1
UniProt Synonym Gene Names
CNSA1; SH3D2B; EEN
UniProt Entry Name
SH3G1_HUMAN
NCBI Protein Information
endophilin-A2

NCBI Summary for SH3GL1

This gene encodes a member of the endophilin family of Src homology 3 domain-containing proteins. The encoded protein is involved in endocytosis and may also play a role in the cell cycle. Overexpression of this gene may play a role in leukemogenesis, and the encoded protein has been implicated in acute myeloid leukemia as a fusion partner of the myeloid-lymphoid leukemia protein. Pseudogenes of this gene are located on the long arm of chromosomes 11 and 17. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Jan 2011]
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UniProt Comments for SH3GL1

SH3GL1: Implicated in endocytosis. May recruit other proteins to membranes with high curvature. In some cases of acute leukemia, a translocation results in the formation of a MLL-EEN fusion gene. Belongs to the endophilin family.

Protein type: Vesicle; Oncoprotein

Chromosomal Location of Human Ortholog: 19p13.3

Cellular Component: cell junction; cell projection; cytoplasm; early endosome membrane; podosome

Molecular Function: GTPase binding; identical protein binding; lipid binding; phosphatase binding; protein binding

Biological Process: central nervous system development; endocytosis; signal transduction

Disease: Leukemia, Acute Myeloid
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-SH3GL1 antibody

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