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NPAS4 Antibody – Goat Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS422570

MBS422570 is an antibody targeting NPAS4. Also known as Goat anti-NPAS4 Antibody. Reactive in Tested: Human, Mouse, Rat; Expected from sequence similarity: Human, Mouse, Rat, Dog, Cow. Host: Goat. 266743 More details

$450 / 0.1 mg +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
NPAS4, Polyclonal Antibody
Full Product Name
Goat anti-NPAS4 Antibody
Product Gene Name
Product Synonym Names
NPAS4; neuronal PAS domain protein 4; Le-PAS; NXF; PASD10; bHLHe79; HLH-PAS transcription factor NXF; PAS domain-containing protein 10; class E basic helix-loop-helix protein 79; neuronal PAS domain-containing protein 4; neuronal PAS4; NPAS4 antibody; neuronal PAS domain protein 4 antibody; Le-PAS antibody; NXF antibody; PASD10 antibody; bHLHe79 antibody; HLH-PAS transcription factor NXF antibody; PAS domain-containing protein 10 antibody; class E basic helix-loop-helix protein 79 antibody; neuronal PAS4 antibody
Immunogen Sequence
CRFNTSKSLR RQS
Clonality
Polyclonal
Host
Goat
Species Reactivity
Tested: Human, Mouse, Rat; Expected from sequence similarity: Human, Mouse, Rat, Dog, Cow
Form/Format
Supplied at 0.5 mg/ml in Tris saline, 0. 02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Purity/Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Concentration
100ug specific antibody in 200ul (lot specific)
Preparation and Storage
Aliquot and store at -20 degree C. Minimize freezing and thawing.
Antibody/Peptide Pairs
NPAS4 peptide (MBS427576) is used for blocking the activity of NPAS4 antibody (MBS422570)
OMIM
608554
Immunogen
Peptide with sequence CRFNTSKSLRRQS, from the internal region of the protein sequence according to NP_849195.2.
ISO Certification
Manufactured in an ISO 9001:2008 Certified Laboratory.
Applications Tested/Suitable for anti-NPAS4 antibody
Peptide ELISA (EIA), Western Blot (WB)
Application Notes for anti-NPAS4 antibody
Peptide ELISA: Antibody detection limit dilution 1: 4000.
Western Blot: Approx 90kDa band observed in Human Brain (Frontal Cortex) lysates and in Mouse and Rat Brain lysates (calculated MW of 87.1kDa according to NP_849195.2). Recommended concentration: 0.3-1ug/ml.

Western Blot (WB) of anti-NPAS4 antibody

(0.3ug/ml) staining of Human Frontal Cortex lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Goat Anti-NPAS4 Polyclonal Antibody – Cat# MBS422570

Western Blot (WB) of anti-NPAS4 antibody

(1ug/ml) staining of Mouse (A) and Rat (B) Brain lysates (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Goat Anti-NPAS4 Polyclonal Antibody, Western Blot (WB) – Cat# MBS422570

Other Notes

Small volumes of anti-NPAS4 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for NPAS4. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Secondary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
neuronal PAS domain-containing protein 4 isoform 1
NCBI Official Synonym Full Names
neuronal PAS domain protein 4
NCBI Official Symbol
NPAS4
NCBI Official Synonym Symbols
NXF; Le-PAS; PASD10; bHLHe79
UniProt Protein Name
Neuronal PAS domain-containing protein 4
UniProt Synonym Protein Names
Class E basic helix-loop-helix protein 79; bHLHe79; HLH-PAS transcription factor NXF; PAS domain-containing protein 10
UniProt Gene Name
NPAS4
UniProt Synonym Gene Names
BHLHE79; NXF; PASD10; Neuronal PAS4; bHLHe79
UniProt Entry Name
NPAS4_HUMAN
NCBI Protein Information
neuronal PAS domain-containing protein 4

NCBI Summary for NPAS4

NXF is a member of the basic helix-loop-helix-PER (MIM 602260)-ARNT (MIM 126110)-SIM (see SIM2; MIM 600892) (bHLH-PAS) class of transcriptional regulators, which are involved in a wide range of physiologic and developmental events (Ooe et al., 2004 [PubMed 14701734]).[supplied by OMIM, Mar 2008]

UniProt Comments for NPAS4

NPAS4: Acts as a transcriptional activator in the presence of ARNT. Can activate the CME (CNS midline enhancer) element and the expression of the drebrin gene.

Protein type: DNA-binding

Chromosomal Location of Human Ortholog: 11q13

Cellular Component: nucleus; transcription factor complex

Molecular Function: protein heterodimerization activity

Biological Process: positive regulation of transcription from RNA polymerase II promoter; regulation of inhibitory postsynaptic membrane potential; transcription from RNA polymerase II promoter
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Organs/Tissues associated with anti-NPAS4 antibody

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