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IRF2BP1 Antibody – Goat Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS422591

MBS422591 is an antibody targeting IRF2BP1. Also known as Goat anti-IRF2BP1 (aa109-122) Antibody. Reactive in Tested: Human, Mouse; Expected from sequence similarity: Human, Mouse, Rat, Dog. Host: Goat. More details

$450 / 0.1 mg +1 FREE 8GB USB
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Product Overview

Online Datasheet in PDF
Product Name
IRF2BP1, Polyclonal Antibody
Full Product Name
Goat anti-IRF2BP1 (aa109-122) Antibody
Product Gene Name
Product Synonym Names
Goat Anti-IRF2BP1 (aa461-474) Antibody; IRF2BP1; interferon regulatory factor 2 binding protein 1; DKFZp434M154; IRF2BP1 antibody; interferon regulatory factor 2 binding protein 1 antibody; DKFZp434M154 antibody; IRF2BP1 (aa109-122)
Immunogen Sequence
QDRYDRATSS GRLP
Clonality
Polyclonal
Host
Goat
Species Reactivity
Tested: Human, Mouse; Expected from sequence similarity: Human, Mouse, Rat, Dog
Form/Format
Supplied at 0.5 mg/ml in Tris saline, 0. 02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Purity/Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Concentration
100ug specific antibody in 200ul (lot specific)
Preparation and Storage
Aliquot and store at -20 degree C. Minimize freezing and thawing.
Antibody/Peptide Pairs
IRF2BP1 peptide (MBS428288) is used for blocking the activity of IRF2BP1 antibody (MBS422591)
Molecular Weight
61,688 Da
OMIM
615331
Immunogen
Peptide with sequence C-QDRYDRATSSGRLP, from the internal region of the protein sequence according to NP_056464.1.
Epitope
Internal region
ISO Certification
Manufactured in an ISO 9001:2008 Certified Laboratory.
Applications Tested/Suitable for anti-IRF2BP1 antibody
Peptide ELISA (EIA), Western Blot (WB)
Application Notes for anti-IRF2BP1 antibody
Peptide ELISA: Antibody detection limit dilution 1: 32000.
Western Blot: Approx 75kDa band observed in lysates of cell line HepG2 and 80kDa in nuclear lysates of cell line NIH3T3 (calculated MW of 61.7kDa according to NP_056464.1). Recommended concentration: 0.1-0.3ug/ml.

Western Blot (WB) of anti-IRF2BP1 antibody

(0.1ug/ml) staining of HepG2 lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Goat Anti-IRF2BP1 Polyclonal Antibody – Cat# MBS422591

Western Blot (WB) of anti-IRF2BP1 antibody

(0.3ug/ml) staining of nuclear NIH3T3 lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Goat Anti-IRF2BP1 Polyclonal Antibody, Western Blot (WB) – Cat# MBS422591

Other Notes

Small volumes of anti-IRF2BP1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for IRF2BP1. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Secondary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
interferon regulatory factor 2-binding protein 1
NCBI Official Synonym Full Names
interferon regulatory factor 2 binding protein 1
NCBI Official Symbol
IRF2BP1
UniProt Protein Name
Interferon regulatory factor 2-binding protein 1
UniProt Synonym Protein Names
Probable E3 ubiquitin-protein ligase IRF2BP1 (EC:6.3.2.-)
UniProt Gene Name
IRF2BP1
UniProt Synonym Gene Names
IRF-2-binding protein 1; IRF-2BP1
UniProt Entry Name
I2BP1_HUMAN
NCBI Protein Information
interferon regulatory factor 2-binding protein 1

UniProt Comments for IRF2BP1

IRF2BP1: Acts as a transcriptional corepressor in a IRF2- dependent manner; this repression is not mediated by histone deacetylase activities. May act as an E3 ligase towards JDP2, enhancing its polyubiquitination. Represses ATF2-dependent transcriptional activation. Belongs to the IRF2BP family.

Protein type: Transcription, coactivator/corepressor; EC 6.3.2.-

Chromosomal Location of Human Ortholog: 19q13.32

Cellular Component: nucleoplasm

Molecular Function: ligase activity; metal ion binding; protein binding

Biological Process: regulation of transcription, DNA-dependent; transcription, DNA-dependent
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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