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IDH3A Antibody – Goat Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS422653

MBS422653 is an antibody targeting IDH3A. Also known as Goat anti-IDH3A Antibody. Reactive in Tested: Human, Mouse, Rat, Pig; Expected from sequence similarity: Human, Mouse, Rat, Dog, Pig, Cow. Host: Goat. More details

$450 / 0.1 mg +1 FREE 8GB USB
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Product Overview

Online Datasheet in PDF
Product Name
IDH3A, Polyclonal Antibody
Full Product Name
Goat anti-IDH3A Antibody
Product Gene Name
anti-IDH3A antibody
Product Synonym Names
IDH3A; isocitrate dehydrogenase 3 (NAD+) alpha; H-IDH alpha; NAD(+)-specific ICDH subunit alpha; NAD(H)-specific isocitrate dehydrogenase alpha subunit; NAD+-specific ICDH; isocitrate dehydrogenase (NAD+) alpha chain; isocitrate dehydrogenase [NAD] subuni; IDH3A antibody; isocitrate dehydrogenase 3 (NAD+) alpha antibody; H-IDH alpha antibody; NAD(+)-specific ICDH subunit alpha antibody; NAD(H)-specific isocitrate dehydrogenase alpha subunit antibody; NAD+-specific ICDH antibody; isocitrate dehydrogenase (NAD+) alpha chain antibody; isocitrate dehydrogenase [NAD] subunit alpha; mitochondrial antibody; isocitric dehydrogenase subunit alpha antibody
Immunogen Sequence
DFTEEICRRV KDLD
Clonality
Polyclonal
Host
Goat
Species Reactivity
Tested: Human, Mouse, Rat, Pig; Expected from sequence similarity: Human, Mouse, Rat, Dog, Pig, Cow
Form/Format
Supplied at 0.5 mg/ml in Tris saline, 0. 02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Purity/Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Concentration
100ug specific antibody in 200ul (lot specific)
Preparation and Storage
Aliquot and store at -20 degree C. Minimize freezing and thawing.
Antibody/Peptide Pairs
IDH3A peptide (MBS427412) is used for blocking the activity of IDH3A antibody (MBS422653)
Molecular Weight
31,381 Da
OMIM
601149
Immunogen
Peptide with sequence DFTEEICRRVKDLD, from the C Terminus of the protein sequence according to NP_005521.1.
ISO Certification
Manufactured in an ISO 9001:2008 Certified Laboratory.
Applications Tested/Suitable for anti-IDH3A antibody
Peptide ELISA (EIA), Western Blot (WB)
Application Notes for anti-IDH3A antibody
Peptide ELISA: Antibody detection limit dilution 1: 32000.
Western Blot: Approx 37kDa band observed in lysates of Human Lymph Nodes, and of cell line DAUDI, and 38kDa in Human, Mouse and Rat Spleen lysates and in Pig Spleen and Skeletal Muscle lysates (calculated MW of 39.6kDa according to NP_005521.1). Recommended concentration: 0.1-0.3ug/ml. Primary incubation was 1 hour.
IHC: Paraffin embedded Human Thymus. Recommended concentration: 3.75µg/ml.

Western Blot (WB) of anti-IDH3A antibody

(0.1ug/ml) staining of Human Lymph Nodes lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Goat Anti-IDH3A Polyclonal Antibody – Cat# MBS422653

Western Blot (WB) of anti-IDH3A antibody

(0.1ug/ml) staining of Human (A), Mouse (B) and Rat (C) Spleen lysates (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Goat Anti-IDH3A Polyclonal Antibody, Western Blot (WB) – Cat# MBS422653

Western Blot (WB) of anti-IDH3A antibody

(0.3ug/ml) staining of Pig Spleen (A) and Pig Skeletal Muscle (B) lysates (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Goat Anti-IDH3A Polyclonal Antibody, Western Blot (WB) – Cat# MBS422653

Immunohistochemistry (IHC) of anti-IDH3A antibody

3.75µg/ml) staining of paraffin embedded Human Thymus. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
Goat Anti-IDH3A Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS422653

Other Notes

Small volumes of anti-IDH3A antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for IDH3A. It may not necessarily be applicable to this product.

NCBI GI #
5031777
NCBI GeneID
3419
NCBI Accession #
NP_005521.1
UniProt Secondary Accession #
Q9H3X0; D3DW83
UniProt Related Accession #
P50213
GenBank Nucleotide #
NM_005530.2
NCBI Official Full Name
isocitrate dehydrogenase
NCBI Official Synonym Full Names
isocitrate dehydrogenase 3 (NAD(+)) alpha
NCBI Official Symbol
IDH3A
UniProt Protein Name
Isocitrate dehydrogenase [NAD] subunit alpha, mitochondrial
UniProt Synonym Protein Names
Isocitric dehydrogenase subunit alpha; NAD(+)-specific ICDH subunit alpha
Protein Family
Isocitrate dehydrogenase
UniProt Gene Name
IDH3A
UniProt Entry Name
IDH3A_HUMAN
NCBI Protein Information
isocitrate dehydrogenase [NAD] subunit alpha, mitochondrial

NCBI Summary for IDH3A

Isocitrate dehydrogenases catalyze the oxidative decarboxylation of isocitrate to 2-oxoglutarate. These enzymes belong to two distinct subclasses, one of which utilizes NAD(+) as the electron acceptor and the other NADP(+). Five isocitrate dehydrogenases have been reported: three NAD(+)-dependent isocitrate dehydrogenases, which localize to the mitochondrial matrix, and two NADP(+)-dependent isocitrate dehydrogenases, one of which is mitochondrial and the other predominantly cytosolic. NAD(+)-dependent isocitrate dehydrogenases catalyze the allosterically regulated rate-limiting step of the tricarboxylic acid cycle. Each isozyme is a heterotetramer that is composed of two alpha subunits, one beta subunit, and one gamma subunit. The protein encoded by this gene is the alpha subunit of one isozyme of NAD(+)-dependent isocitrate dehydrogenase. [provided by RefSeq, Jul 2008]
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UniProt Comments for IDH3A

IDH3A: a enzyme that that catalyzes the oxidative decarboxylation of isocitrate to alpha-ketoglutarate (alpha-KG), requiring nicotinamide adenine dinucleotide (NAD) as a cofactor. The holoenzyme is a heterooligomer of subunits alpha, beta, and gamma in the apparent ratio of 2:1:1. IDH3 is a mitochondrial enzyme that functions in the Krebs cycle, which generates substrates for cellular respiration. Alpha-KG can exit the mitochondria and enter other cellular compartments where it is a cofactor for the dioxygenases that hydroxylate the transcription factor HIF and trigger its degradation by VHL. Since HIF turns on oncogenic pathways, IDH3 has apparent tumor suppressor activity. Two isoforms of the human protein are produced by alternative splicing.

Protein type: Mitochondrial; Carbohydrate Metabolism - citrate (TCA) cycle; Oxidoreductase; EC 1.1.1.41

Chromosomal Location of Human Ortholog: 15q25.1-q25.2

Cellular Component: mitochondrial matrix; mitochondrion; myelin sheath; nucleus

Molecular Function: isocitrate dehydrogenase (NAD+) activity; magnesium ion binding; NAD binding

Biological Process: carbohydrate metabolic process; tricarboxylic acid cycle
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Research Articles on IDH3A

1. The silencing of IDH3a significantly delayed tumor growth by suppressing the HIF-1-mediated Warburg effect and angiogenesis.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Pathways associated with anti-IDH3A antibody

 Products by Pathway  Pathway Diagram
 2-Oxocarboxylic Acid Metabolism Pathway antibodies  2-Oxocarboxylic Acid Metabolism Pathway Diagram
 2-Oxocarboxylic Acid Metabolism Pathway antibodies  2-Oxocarboxylic Acid Metabolism Pathway Diagram
 Biosynthesis Of Amino Acids Pathway antibodies  Biosynthesis Of Amino Acids Pathway Diagram
 Biosynthesis Of Amino Acids Pathway antibodies  Biosynthesis Of Amino Acids Pathway Diagram
 Carbon Metabolism Pathway antibodies  Carbon Metabolism Pathway Diagram
 Carbon Metabolism Pathway antibodies  Carbon Metabolism Pathway Diagram
 Citrate Cycle (TCA Cycle) Pathway antibodies  Citrate Cycle (TCA Cycle) Pathway Diagram
 Citrate Cycle (TCA Cycle) Pathway antibodies  Citrate Cycle (TCA Cycle) Pathway Diagram
 Citrate Cycle (TCA Cycle, Krebs Cycle) Pathway antibodies  Citrate Cycle (TCA Cycle, Krebs Cycle) Pathway Diagram
 Citrate Cycle (TCA Cycle, Krebs Cycle) Pathway antibodies  Citrate Cycle (TCA Cycle, Krebs Cycle) Pathway Diagram

Diseases associated with anti-IDH3A antibody

 Disease Name  Pubmed Publications
 Nervous System Diseases Antibodies  >4 publications with IDH3A and Nervous System Diseases
 Disease Models, Animal Antibodies  >3 publications with IDH3A and Disease Models, Animal
 Brain Diseases Antibodies  >3 publications with IDH3A and Brain Diseases
 Cardiovascular Diseases Antibodies  >2 publications with IDH3A and Cardiovascular Diseases
 Hypertension Antibodies  >1 publications with IDH3A and Hypertension
 Breast Neoplasms Antibodies  >1 publications with IDH3A and Breast Neoplasms
 Chromosome Aberrations Antibodies  >1 publications with IDH3A and Chromosome Aberrations
 Fatty Liver Antibodies  >1 publications with IDH3A and Fatty Liver
 Cognition Disorders Antibodies  >1 publications with IDH3A and Cognition Disorders
 Kidney Diseases Antibodies  >1 publications with IDH3A and Kidney Diseases

Organs/Tissues associated with anti-IDH3A antibody

 Organ/Tissue Name  Pubmed Publications
 Brain Antibodies  >5 publications with IDH3A and Brain
 Blood Antibodies  >3 publications with IDH3A and Blood
 Liver Antibodies  >3 publications with IDH3A and Liver
 Kidney Antibodies  >3 publications with IDH3A and Kidney
 Heart Antibodies  >2 publications with IDH3A and Heart
 Bone Antibodies  >1 publications with IDH3A and Bone
 Muscle Antibodies  >1 publications with IDH3A and Muscle
 Thyroid Antibodies  >1 publications with IDH3A and Thyroid
 Vascular Antibodies  >1 publications with IDH3A and Vascular
 Lung Antibodies  >1 publications with IDH3A and Lung
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