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NUP84 Antibody – Goat Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS422722

MBS422722 is an antibody targeting NUP84. Also known as Goat anti-NUP84 (yeast) Antibody. Reactive in Tested: S. cerevisiae; Expected from sequence similarity: Saccharomyces cerevisiae S288c. Host: Goat. More details

$450 / 0.1 mg +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
NUP84, Polyclonal Antibody
Full Product Name
Goat anti-NUP84 (yeast) Antibody
Product Gene Name
Product Synonym Names
NUP84; Nup84p; YDL116W; Nuclear pore protein NUP84; Nucleoporin NUP84; NUP84 antibody; Nup84p antibody; YDL116W antibody; Nuclear pore protein NUP84 antibody; Nucleoporin NUP84 antibody; NUP84 (yeast)
Immunogen Sequence
ELSPTYQTER FTK
Clonality
Polyclonal
Host
Goat
Species Reactivity
Tested: S. cerevisiae; Expected from sequence similarity: Saccharomyces cerevisiae S288c
Form/Format
Supplied at 0.5 mg/ml in Tris saline, 0. 02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Purity/Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Concentration
100ug specific antibody in 200ul (lot specific)
Preparation and Storage
Aliquot and store at -20 degree C. Minimize freezing and thawing.
Antibody/Peptide Pairs
NUP84 peptide (MBS427559) is used for blocking the activity of NUP84 antibody (MBS422722)
Molecular Weight
83,635 Da
Immunogen
Peptide with sequence ELSPTYQTERFTK-C, from the N Terminus of the protein sequence according to NP_010167.1.
Epitope
N Terminus
ISO Certification
Manufactured in an ISO 9001:2008 Certified Laboratory.
Applications Tested/Suitable for anti-NUP84 antibody
Peptide ELISA (EIA), Western Blot (WB)
Application Notes for anti-NUP84 antibody
Peptide ELISA: Antibody detection limit dilution 1: 8000.
Western Blot: Approx 75kDa band observed in S. cerevisiae wildtype lysates but not in the knock-out strain (calculated MW of 83.6kDa according to NP_010167.1). Recommended concentration: 2-4ug/ml.

Western Blot (WB) of anti-NUP84 antibody

(2ug/ml) staining of Saccharomyces cerevisiae S288c lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence. Data kindly provided by Dr. F Reggiori, University of Utrecht, Netherlands
Goat Anti-NUP84 Polyclonal Antibody – Cat# MBS422722

Other Notes

Small volumes of anti-NUP84 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for NUP84. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Secondary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
Nup84p
NCBI Official Symbol
NUP84
UniProt Protein Name
Nucleoporin NUP84
UniProt Synonym Protein Names
Nuclear pore protein NUP84
Protein Family
UniProt Gene Name
NUP84
UniProt Entry Name
NUP84_YEAST
NCBI Protein Information
Nup84p

UniProt Comments for NUP84

Functions as a component of the nuclear pore complex (NPC). NPC components, collectively referred to as nucleoporins (NUPs), can play the role of both NPC structural components and of docking or interaction partners for transiently associated nuclear transport factors. NUP84 is involved in nuclear poly(A)+ RNA export, in NPC assembly and distribution, as well as in nuclear envelope organization.
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Pathways associated with anti-NUP84 antibody

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