Goat Anti-HRH2 Polyclonal Antibody – Cat# MBS422789

(0.1ug/ml) staining of Duodenum lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Histamine Receptor H2 (HRH2) Antibody – Goat Polyclonal

Name: HRH2 Antibody; Polyclonal; Goat; Human-Reactive; MyBioSource
Brand: MyBioSource
SKU: MBS422789
Price: 450 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS422789

MBS422789 is an antibody targeting HRH2. Also known as Goat anti-Histamine Receptor H2 (aa309-323) Antibody. Reactive in Tested: Human; Expected from sequence similarity: Human. Host: Goat. More details

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Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Product Name

Histamine Receptor H2 (HRH2), Polyclonal Antibody

Full Product Name

Goat anti-Histamine Receptor H2 (aa309-323) Antibody

Product Gene Name

anti-HRH2 antibody

Product Synonym Names

Goat Anti-Histamine Receptor H2 Antibody; HRH2; histamine receptor H2; H2R; HH2R; gastric receptor 1; gastric receptor I; histamine H2 receptor; HRH2 antibody; histamine receptor H2 antibody; H2R antibody; HH2R antibody; gastric receptor 1 antibody; gastric receptor I antibody; histamine H2 receptor antibody; gastric receptor 1 antibody; gastric receptor I antibody; H2R antibody; HH2R antibody; histamine H2 receptor antibody; histamine receptor H2 antibody; HRH2 antibody; Histamine Receptor H2; Histamine Receptor H2 (aa309-323)

Immunogen Sequence

SHNSHKTSLR LNNS

Clonality

Polyclonal

Host

Goat

Species Reactivity

Tested: Human; Expected from sequence similarity: Human

Form/Format

Supplied at 0.5 mg/ml in Tris saline, 0. 02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

Purity/Purification

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Concentration

100ug specific antibody in 200ul (lot specific)

Preparation and Storage

Aliquot and store at -20 degree C. Minimize freezing and thawing.

Molecular Weight

44,541 Da

OMIM

142703

Immunogen

Peptide with sequence C-SHNSHKTSLRLNNS, from the internal region of the protein sequence according to NP_001124527.1; NP_071640.1.

Epitope

Internal region

Note

This antibody is expected to recognize both reported isoforms (NP_001124527.1; NP_071640.1).

ISO Certification

Manufactured in an ISO 9001:2008 Certified Laboratory.

Applications Tested/Suitable for anti-HRH2 antibody

Peptide ELISA (EIA), Western Blot (WB)

Application Notes for anti-HRH2 antibody

Peptide ELISA: Antibody detection limit dilution 1: 32000.
Western Blot: Approx 45kDa band observed in Human Duodenum and Ileum lysates (calculated MW of 44.5kDa according to NP_001124527.1). Recommended concentration: 0.1-0.3ug/ml.

Other Notes

Small volumes of anti-HRH2 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for HRH2. It may not necessarily be applicable to this product.

NCBI GI #

197085610

NCBI GeneID

3274

NCBI Accession #

NP_001124527.1

NCBI Related Accession #

NP_071640.1

UniProt Secondary Accession #

Q14464; Q7Z5R9; B5BUP7

UniProt Related Accession #

P25021

GenBank Nucleotide #

NM_001131055.1

NCBI Official Full Name

histamine H2 receptor isoform 1

NCBI Official Synonym Full Names

histamine receptor H2

NCBI Official Symbol

HRH2

NCBI Official Synonym Symbols

H2R

UniProt Protein Name

Histamine H2 receptor

UniProt Synonym Protein Names

Gastric receptor I

Protein Family

Histamine H2 receptor

UniProt Gene Name

HRH2

UniProt Synonym Gene Names

H2R; HH2R

UniProt Entry Name

HRH2_HUMAN

NCBI Protein Information

histamine H2 receptor

NCBI Summary for HRH2

Histamine is a ubiquitous messenger molecule released from mast cells, enterochromaffin-like cells, and neurons. Its various actions are mediated by histamine receptors H1, H2, H3 and H4. Histamine receptor H2 belongs to the family 1 of G protein-coupled receptors. It is an integral membrane protein and stimulates gastric acid secretion. It also regulates gastrointestinal motility and intestinal secretion and is thought to be involved in regulating cell growth and differentiation. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Aug 2008]

UniProt Comments for HRH2

H2R: The H2 subclass of histamine receptors mediates gastric acid secretion. Also appears to regulate gastrointestinal motility and intestinal secretion. Possible role in regulating cell growth and differentiation. The activity of this receptor is mediated by G proteins which activate adenylyl cyclase and, through a separate G protein-dependent mechanism, the phosphoinositide/protein kinase (PKC) signaling pathway. Belongs to the G-protein coupled receptor 1 family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral; GPCR, family 1; Membrane protein, multi-pass; Receptor, GPCR

Chromosomal Location of Human Ortholog: 5q35.2

Cellular Component: integral to plasma membrane; plasma membrane

Molecular Function: histamine receptor activity

Biological Process: G-protein signaling, coupled to cyclic nucleotide second messenger; gastrin-induced gastric acid secretion; gland development; gut development; histamine-induced gastric acid secretion; immune response; memory; positive regulation of vasoconstriction; regulation of synaptic plasticity; visual learning

Research Articles on HRH2

1. Thus, the results in the initial study were due to the degradation of histamine in skeletal muscle by ascorbate, because the histaminergic vasodilatation was unaffected by N-acetylcysteine.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Pathways associated with anti-HRH2 antibody

Products by Pathway	Pathway Diagram
Amine Ligand-binding Receptors Pathway antibodies	Amine Ligand-binding Receptors Pathway Diagram
Calcium Signaling Pathway antibodies	Calcium Signaling Pathway Diagram
Calcium Signaling Pathway antibodies	Calcium Signaling Pathway Diagram
Class A/1 (Rhodopsin-like Receptors) Pathway antibodies	Class A/1 (Rhodopsin-like Receptors) Pathway Diagram
G Alpha (s) Signalling Events Pathway antibodies	G Alpha (s) Signalling Events Pathway Diagram
GPCR Downstream Signaling Pathway antibodies	GPCR Downstream Signaling Pathway Diagram
GPCR Ligand Binding Pathway antibodies	GPCR Ligand Binding Pathway Diagram
GPCRs, Class A Rhodopsin-like Pathway antibodies	GPCRs, Class A Rhodopsin-like Pathway Diagram
Gastric Acid Secretion Pathway antibodies	Gastric Acid Secretion Pathway Diagram
Gastric Acid Secretion Pathway antibodies	Gastric Acid Secretion Pathway Diagram

Diseases associated with anti-HRH2 antibody

Disease Name	Pubmed Publications
Drug Hypersensitivity Antibodies	>3 publications with HRH2 and Drug Hypersensitivity
Inflammation Antibodies	>3 publications with HRH2 and Inflammation
Pain Antibodies	>2 publications with HRH2 and Pain
Urticaria Antibodies	>2 publications with HRH2 and Urticaria
Seizures Antibodies	>2 publications with HRH2 and Seizures
Neoplasms, Experimental Antibodies	>2 publications with HRH2 and Neoplasms, Experimental
Ulcer Antibodies	>1 publications with HRH2 and Ulcer
Kidney Diseases Antibodies	>1 publications with HRH2 and Kidney Diseases
Peptic Ulcer Antibodies	>1 publications with HRH2 and Peptic Ulcer

Organs/Tissues associated with anti-HRH2 antibody

Organ/Tissue Name	Pubmed Publications
Brain Antibodies	>4 publications with HRH2 and Brain
Skin Antibodies	>2 publications with HRH2 and Skin
Kidney Antibodies	>1 publications with HRH2 and Kidney