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DAPP1 Antibody – Goat Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS422886

MBS422886 is an antibody targeting DAPP1. Also known as Goat anti-DAPP1/BAM32 Antibody. Reactive in Tested: Human; Expected from sequence similarity: Human, Dog, Cow. Host: Goat. More details

$450 / 0.1 mg +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
DAPP1/BAM32, Polyclonal Antibody
Full Product Name
Goat anti-DAPP1/BAM32 Antibody
Product Gene Name
Product Synonym Gene Name
BAM32
Product Synonym Names
Goat Anti-DAPP1 Antibody; DAPP1; dual adaptor of phosphotyrosine and 3-phosphoinositides; BAM32; DKFZp667E0716; DAPP1 antibody; dual adaptor of phosphotyrosine and 3-phosphoinositides antibody; BAM32 antibody; DKFZp667E0716 antibody; DAPP1 / BAM32
Immunogen Sequence
KLSQIRKQLN QGE
Clonality
Polyclonal
Host
Goat
Species Reactivity
Tested: Human; Expected from sequence similarity: Human, Dog, Cow
Form/Format
Supplied at 0.5 mg/ml in Tris saline, 0. 02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Purity/Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Concentration
100ug specific antibody in 200ul (lot specific)
Preparation and Storage
Aliquot and store at -20 degree C. Minimize freezing and thawing.
Antibody/Peptide Pairs
Molecular Weight
32,044 Da
OMIM
605768
Immunogen
Peptide with sequence C-KLSQIRKQLNQGE, from the internal region (near C Terminus) of the protein sequence according to NP_055210.2.
Epitope
Internal region (near C Terminus)
ISO Certification
Manufactured in an ISO 9001:2008 Certified Laboratory.
Applications Tested/Suitable for anti-DAPP1 antibody
Peptide ELISA (EIA), Western Blot (WB-Trf)
Application Notes for anti-DAPP1 antibody
Peptide ELISA: Antibody detection limit dilution 1: 8000.
Western Blot: In transfected HEK293 transiently expressing full-length Human DAPP1 (myc and DYKDDDDK tagged), a band of approx.35kDa was observed. No bands were observed in mock-transfected HEK293 and the same band was observed using anti-DYKDDDDK tag antibody. Recommended concentration, 0.1- 0.3ug/ml.

Western Blot (WB) of anti-DAPP1 antibody

HEK293 lysate (10ug protein in RIPA buffer) over expressing Human DAPP1 with DYKDDDDK tag probed (0.1ug/ml) in Lane A and probed with anti- DYKDDDDK Tag (1/3000) in lane C. Mock-transfected HEK293 probed (1mg/ml) in Lane B. Primary incubations were for 1 hour. Detected by chemiluminescence.
Goat Anti-DAPP1 Polyclonal Antibody – Cat# MBS422886

Other Notes

Small volumes of anti-DAPP1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for DAPP1. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Secondary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
dual adapter for phosphotyrosine and 3-phosphotyrosine and 3-phosphoinositide isoform 1
NCBI Official Synonym Full Names
dual adaptor of phosphotyrosine and 3-phosphoinositides
NCBI Official Symbol
DAPP1
NCBI Official Synonym Symbols
BAM32
UniProt Protein Name
Dual adapter for phosphotyrosine and 3-phosphotyrosine and 3-phosphoinositide
UniProt Synonym Protein Names
B lymphocyte adapter protein Bam32; B-cell adapter molecule of 32 kDa
UniProt Gene Name
DAPP1
UniProt Synonym Gene Names
BAM32; hDAPP1
UniProt Entry Name
DAPP1_HUMAN
NCBI Protein Information
dual adapter for phosphotyrosine and 3-phosphotyrosine and 3-phosphoinositide

UniProt Comments for DAPP1

DAPP1: an adaptor protein. Regulates antigen receptor signaling downstream of phosphatidylinositol 3-kinase.

Protein type: Adaptor/scaffold

Chromosomal Location of Human Ortholog: 4q25-q27

Cellular Component: cytosol; plasma membrane

Molecular Function: phosphatidylinositol-3,4,5-triphosphate binding; phosphatidylinositol-3,4-bisphosphate binding; phospholipid binding; protein binding

Biological Process: protein amino acid dephosphorylation; signal transduction
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-DAPP1 antibody

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