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ZNRF1 Antibody – Goat Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS422965

MBS422965 is an antibody targeting Znrf1. Also known as Goat anti-ZNRF1 (mouse) Antibody. Reactive in Tested: Mouse; Expected from sequence similarity: Human, Mouse, Rat, Dog, Pig, Cow. Host: Goat. More details

$450 / 0.1 mg +1 FREE 8GB USB
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Testing Data View PDF
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Technical Datasheet View PDF
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Product Overview

Online Datasheet in PDF
Product Name
ZNRF1, Polyclonal Antibody
Full Product Name
Goat anti-ZNRF1 (mouse) Antibody
Product Gene Name
anti-Znrf1 antibody
Product Synonym Names
Znrf1; zinc and ring finger 1; B830022L21Rik; MGC101991; Rnf42; Zrfp1; nin283; E3 ubiquitin-protein ligase ZNRF1; nerve injury-induced gene 283 protein; peripheral nerve injury protein nin283; ring finger protein 42; zinc ring finger protein 1; zinc/RING; B830022L21Rik antibody; E3 ubiquitin-protein ligase ZNRF1 antibody; MGC101991 antibody; nerve injury-induced gene 283 protein antibody; nin283 antibody; peripheral nerve injury protein nin283 antibody; ring finger protein 42 antibody; Rnf42 antibody; zinc and ring finger 1 antibody; zinc ring finger protein 1 antibody; zinc/RING finger protein 1 antibody; Znrf1 antibody; Zrfp1 antibody; ZNRF1 (mouse)
Immunogen Sequence
SDSTYAHGNG YQET
Clonality
Polyclonal
Host
Goat
Species Reactivity
Tested: Mouse; Expected from sequence similarity: Human, Mouse, Rat, Dog, Pig, Cow
Form/Format
Supplied at 0.5 mg/ml in Tris saline, 0. 02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Purity/Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Concentration
100ug specific antibody in 200ul (lot specific)
Preparation and Storage
Aliquot and store at -20 degree C. Minimize freezing and thawing.
Antibody/Peptide Pairs
ZNRF1 peptide (MBS427873) is used for blocking the activity of ZNRF1 antibody (MBS422965)
Molecular Weight
23,843 Da
Immunogen
Peptide with sequence C-SDSTYAHGNGYQET, from the internal region of the protein sequence according to NP_573469.1; NP_001162093.1.
Epitope
Internal region
Note
This antibody is expected to recognize reported isoforms a and b (NP_573469.1; NP_001162093.1). Reported variants represent identical protein: NP_001162092.1, NP_573469.1
ISO Certification
Manufactured in an ISO 9001:2008 Certified Laboratory.
Applications Tested/Suitable for anti-Znrf1 antibody
Peptide ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC)
Application Notes for anti-Znrf1 antibody
Peptide ELISA: Antibody detection limit dilution 1: 32000.
Immunohistochemistry: In paraffin embedded Mouse Cerebral Cortex shows colabeling with Neurofilament M. Recommended concentration, 10ug/ml. Data obtained from National Institute of Neuroscience, Tokyo, Japan.

Western Blot (WB) of anti-Znrf1 antibody

N2a overexpressing Mouse Znrf1 (mock transfection in first lane) and probed with (1ug/ml), also staining of Mouse Brain lysates (Embryo E14 and adult cerebellum). Primary incubation was 1 hour. Detected by chemiluminescence.
Goat Anti-ZNRF1 Polyclonal Antibody – Cat# MBS422965

Immunofluorescence (IF) of anti-Znrf1 antibody

(10ug/ml) staining of paraffin embedded Mouse Cerebral Cortex. Microwaved antigen retrieval with citrate buffer pH 6, streptavidfine-Alexa 488-staining after biotinylated anti-goat secondary. The Neurofilament M was labeled by Millipore AB1987 (1: 100).
Goat Anti-ZNRF1 Polyclonal Antibody, Immunofluorescence (IF) – Cat# MBS422965

Other Notes

Small volumes of anti-Znrf1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for Znrf1. It may not necessarily be applicable to this product.

NCBI GI #
18875354
NCBI GeneID
170737
NCBI Accession #
NP_573469.1
NCBI Related Accession #
NP_001162093.1
UniProt Secondary Accession #
Q8C5D7
UniProt Related Accession #
Q91V17
GenBank Nucleotide #
NM_133206.3
NCBI Official Full Name
E3 ubiquitin-protein ligase ZNRF1 isoform a
NCBI Official Synonym Full Names
zinc and ring finger 1
NCBI Official Symbol
Znrf1
NCBI Official Synonym Symbols
Rnf42; Zrfp1; nin283; B830022L21Rik
UniProt Protein Name
E3 ubiquitin-protein ligase ZNRF1
UniProt Synonym Protein Names
Nerve injury-induced gene 283 protein; Zinc/RING finger protein 1
Protein Family
E3 ubiquitin-protein ligase
UniProt Gene Name
Znrf1
UniProt Synonym Gene Names
Nin283
UniProt Entry Name
ZNRF1_MOUSE
NCBI Protein Information
E3 ubiquitin-protein ligase ZNRF1

UniProt Comments for Znrf1

ZNRF1: E3 ubiquitin-protein ligase that mediates the ubiquitination of AKT1 and GLUL, thereby playing a role in neuron cells differentiation. Plays a role in the establishment and maintenance of neuronal transmission and plasticity. Regulates Schwann cells differentiation by mediating ubiquitination of GLUL. Promotes neurodegeneration by mediating 'Lys-48'-linked polyubiquitination and subsequent degradation of AKT1 in axons: degradation of AKT1 prevents AKT1-mediated phosphorylation of GSK3B, leading to GSK3B activation and phosphorylation of DPYSL2/CRMP2 followed by destabilization of microtubule assembly in axons (Probable). 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Ligase; Ubiquitin ligase; EC 6.3.2.-; EC 6.3.2.19; Ubiquitin conjugating system

Cellular Component: cell junction; cytoplasmic vesicle; cytosol; endosome; lysosome; membrane; synapse

Molecular Function: coenzyme F420-0 gamma-glutamyl ligase activity; coenzyme F420-2 alpha-glutamyl ligase activity; ligase activity; metal ion binding; protein binding; ribosomal S6-glutamic acid ligase activity; ubiquitin-protein ligase activity; UDP-N-acetylmuramoylalanyl-D-glutamyl-2,6-diaminopimelate-D-alanyl-D-alanine ligase activity; zinc ion binding

Biological Process: cytoskeleton organization and biogenesis; proteasomal ubiquitin-dependent protein catabolic process
Show more

Research Articles on Znrf1

1. up-regulation of ZNRF1 activity by oxidative stress leads to neuronal apoptosis and Wallerian degeneration.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Pathways associated with anti-Znrf1 antibody

 Products by Pathway  Pathway Diagram
 Adaptive Immune System Pathway antibodies  Adaptive Immune System Pathway Diagram
 Antigen Processing: Ubiquitination & Proteasome Degradation Pathway antibodies  Antigen Processing: Ubiquitination & Proteasome Degradation Pathway Diagram
 Class I MHC Mediated Antigen Processing & Presentation Pathway antibodies  Class I MHC Mediated Antigen Processing & Presentation Pathway Diagram
 Immune System Pathway antibodies  Immune System Pathway Diagram

Diseases associated with anti-Znrf1 antibody

 Disease Name  Pubmed Publications
 Nervous System Diseases Antibodies  >5 publications with Znrf1 and Nervous System Diseases
 Brain Diseases Antibodies  >2 publications with Znrf1 and Brain Diseases
 Disease Models, Animal Antibodies  >1 publications with Znrf1 and Disease Models, Animal

Organs/Tissues associated with anti-Znrf1 antibody

 Organ/Tissue Name  Pubmed Publications
 Muscle Antibodies  >1 publications with Znrf1 and Muscle
 Sympathetic Ganglion Antibodies  >1 publications with Znrf1 and Sympathetic Ganglion
 Eye Antibodies  >1 publications with Znrf1 and Eye
 Brain Antibodies  >1 publications with Znrf1 and Brain
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