• Call +1.858.633.0165 or Fax +1.858.633.0166 or Contact Us

ERK3/MAPK6 (MAPK6) Antibody – Goat Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS422967

MBS422967 is an antibody targeting Mapk6. Also known as Goat anti-ERK3/MAPK6 Antibody. Reactive in Tested: Mouse; Expected from sequence similarity: Human, Mouse, Rat, Dog, Pig, Cow. Host: Goat. More details

$450 / 0.1 mg +1 FREE 8GB USB
Product Documentation
PDF
Sample Manual Insert View PDF
PDF
Testing Data View PDF
PDF
Certificate of Analysis (COA) View PDF
PDF
QC Report View PDF
PDF
Material Safety Data Sheet View PDF
PDF
Technical Datasheet View PDF
PDF
Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
ERK3/MAPK6, Polyclonal Antibody
Full Product Name
Goat anti-ERK3/MAPK6 Antibody
Product Gene Name
Product Synonym Gene Name
ERK3
Product Synonym Names
MAPK6; mitogen-activated protein kinase 6; ERK3; HsT17250; PRKM6; p97MAPK; ERK-3; MAP kinase 6; MAP kinase isoform p97; MAPK 6; extracellular signal-regulated kinase 3; extracellular signal-regulated kinase, p97; p97-MAPK; protein kinase, mitogen-activate; MAPK6 antibody; mitogen-activated protein kinase 6 antibody; ERK3 antibody; HsT17250 antibody; PRKM6 antibody; p97MAPK antibody; ERK-3 antibody; MAP kinase 6 antibody; MAP kinase isoform p97 antibody; MAPK 6 antibody; extracellular signal-regulated kinase 3 antibody; extracellular signal-regulated kinase; p97 antibody; p97-MAPK antibody; protein kinase; mitogen-activated 5 antibody; protein kinase; mitogen-activated 6 antibody; ERK3 / MAPK6
Immunogen Sequence
HSPVGSPLKS IQ
Clonality
Polyclonal
Host
Goat
Species Reactivity
Tested: Mouse; Expected from sequence similarity: Human, Mouse, Rat, Dog, Pig, Cow
Form/Format
Supplied at 0.5 mg/ml in Tris saline, 0. 02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Purity/Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Concentration
100ug specific antibody in 200ul (lot specific)
Preparation and Storage
Aliquot and store at -20 degree C. Minimize freezing and thawing.
Antibody/Peptide Pairs
Molecular Weight
82,681 Da
OMIM
602904
Immunogen
Peptide with sequence C-HSPVGSPLKSIQ, from the internal region (near C Terminus) of the protein sequence according to NP_002739.1.
Epitope
Internal region (near C Terminus)
Note
Reported variants represent identical protein (NP_056621.4; NP_081694.1).
ISO Certification
Manufactured in an ISO 9001:2008 Certified Laboratory.
Applications Tested/Suitable for anti-Mapk6 antibody
Peptide ELISA (EIA), Western Blot (WB-Trf), Immunoprecipitation (IP)
Application Notes for anti-Mapk6 antibody
Peptide ELISA: Antibody detection limit dilution 1: 128000.
Western Blot: In transfected HEK293 transiently expressing Mouse ERK3 / Mapk6 fused to GFP the band with expected size is intensified when co-expressed with MK5 as the stabilizer for ERK3. No bands are observed in transfected HEK293 with empty vector and the same band is observed using anti-GFP antibody. Data obtained from Dr. M. B. Menon, Inst. Biochemistry, Hannover Medical School, Germany. Recommended concentration, 0.5-1ug/ml.

Western Blot (WB) of anti-Mapk6 antibody

HEK293 lysate (10ug protein in RIPA buffer) overexpressing GFP-fused Mouse ERK3 probed with (0.5ug/ml) in left panel and with anti-GFP in right panel. GFP-only exression in the first lane. Primary incubations were for 2 hours. Detected by chemiluminescence.
Goat Anti-MAPK6 Polyclonal Antibody – Cat# MBS422967

Western Blot (WB) of anti-Mapk6 antibody

(1.5ug) immunoprecipitation from lysates of MK5/ERK3 double knockout MEFs, with (third lane) and without (fourth lane) rescued MK5/ERK3 expression through retroviral transduction. The corresponding lysates (first and second lane resp. ) were analyzed in parallel in this Western blot labelled with rabbit anti-Erk3 (and co-precipitation was measured using mouse anti-MK5/PRAK in the lower panel).
Goat Anti-MAPK6 Polyclonal Antibody, Western Blot (WB) – Cat# MBS422967

Other Notes

Small volumes of anti-Mapk6 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for Mapk6. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Secondary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
mitogen-activated protein kinase 6
NCBI Official Synonym Full Names
mitogen-activated protein kinase 6
NCBI Official Symbol
MAPK6
NCBI Official Synonym Symbols
ERK3; PRKM6; p97MAPK; HsT17250
UniProt Protein Name
Mitogen-activated protein kinase 6
UniProt Synonym Protein Names
Extracellular signal-regulated kinase 3; ERK-3; MAP kinase isoform p97; p97-MAPK
UniProt Gene Name
MAPK6
UniProt Synonym Gene Names
ERK3; PRKM6; MAP kinase 6; MAPK 6; ERK-3; p97-MAPK
UniProt Entry Name
MK06_HUMAN
NCBI Protein Information
mitogen-activated protein kinase 6

NCBI Summary for Mapk6

The protein encoded by this gene is a member of the Ser/Thr protein kinase family, and is most closely related to mitogen-activated protein kinases (MAP kinases). MAP kinases also known as extracellular signal-regulated kinases (ERKs), are activated through protein phosphorylation cascades and act as integration points for multiple biochemical signals. This kinase is localized in the nucleus, and has been reported to be activated in fibroblasts upon treatment with serum or phorbol esters. [provided by RefSeq, Jul 2008]
Show more

UniProt Comments for Mapk6

ERK3: a protein kinase of the MAPK family. Plays a critical role in the regulation of cell growth and differentiation. Localized in the nucleus, and has been reported to be activated in fibroblasts upon treatment with serum or phorbol esters.

Protein type: Protein kinase, CMGC; EC 2.7.11.24; Protein kinase, Ser/Thr (non-receptor); Kinase, protein; CMGC group; MAPK family; ERK subfamily; MAPK/ERK subfamily

Chromosomal Location of Human Ortholog: 15q21

Cellular Component: cytoplasm; cytosol; nucleoplasm; nucleus

Molecular Function: ATP binding; MAP kinase activity; protein binding; protein heterodimerization activity; protein kinase binding; protein serine/threonine kinase activity

Biological Process: cell cycle; MAPKKK cascade; protein amino acid phosphorylation; signal transduction
Show more

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Request a Quote

Please fill out the form below and our representative will get back to you shortly.

MBS000000
Contact Us

Please fill out the form below and our representative will get back to you shortly.

MBS000000