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E2F1 Antibody – Goat Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS422994

MBS422994 is an antibody targeting E2F1. Also known as Goat anti-E2F1 (Drosophila, aa480-493) Antibody. Reactive in Tested: Drosophila; Expected from sequence similarity: Drosophila. Host: Goat. More details

$450 / 0.1 mg +1 FREE 8GB USB
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Testing Data View PDF
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Product Overview

Online Datasheet in PDF
Product Name
E2F1, Polyclonal Antibody
Full Product Name
Goat anti-E2F1 (Drosophila, aa480-493) Antibody
Product Gene Name
anti-E2F1 antibody
Product Synonym Names
E2f; E2F transcription factor; Dmel_CG6376; CG6376; DRTF1/E2F; DmE2F-1; Dmel\CG6376; Dp; E(Sev-CycE)3A; E(var)3-93E; E(var)3-95E; E(var)93E; E2F; E2F-1; E2F1; E2f1; Evar(3)164; dE2F; dE2F1; dE2f; dE2f1; de2f1; def21; drosE2F1; e2f; e2f1; l(3)07172; l(3)j3; CG6376 antibody; CG6376-PA antibody; CG6376-PB antibody; CG6376-PC antibody; dE2F antibody; dE2F1 antibody; def21 antibody; DmE2F-1 antibody; Dmel\CG6376 antibody; Dmel_CG6376 antibody; Dp antibody; drosE2F1 antibody; DRTF1/E2F antibody; E(Sev-CycE)3A antibody; E(var)3-93E antibody; E(var)3-95E antibody; E(var)93E antibody; E2f antibody; E2F transcription factor antibody; E2F1 antibody; E2F-1 antibody; E2f-PA antibody; E2f-PB antibody; E2f-PC antibody; E2-promoter binding factor antibody; Evar(3)164 antibody; l(3)07172 antibody; l(3)j3B1 antibody; l(3)j3C2 antibody; l(3)rM729 antibody; E2F1 (Drosophila, aa480-493)
Immunogen Sequence
TKGLFQTPYR SARN
Clonality
Polyclonal
Host
Goat
Species Reactivity
Tested: Drosophila; Expected from sequence similarity: Drosophila
Form/Format
Supplied at 0.5 mg/ml in Tris saline, 0. 02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Purity/Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Concentration
100ug specific antibody in 200ul (lot specific)
Preparation and Storage
Aliquot and store at -20 degree C. Minimize freezing and thawing.
Antibody/Peptide Pairs
E2F1 peptide (MBS427875) is used for blocking the activity of E2F1 antibody (MBS422994)
Molecular Weight
87,460 Da
Immunogen
Peptide with sequence C-TKGLFQTPYRSARN, from the internal region of the protein sequence according to NP_524437.2.
Epitope
Internal region
Note
Reported isoforms represent identical protein: NP_524437.2; NP_732646.1; NP_732647.1
ISO Certification
Manufactured in an ISO 9001:2008 Certified Laboratory.
Applications Tested/Suitable for anti-E2F1 antibody
Peptide ELISA (EIA), Immunofluorescence (IF)
Application Notes for anti-E2F1 antibody
Peptide ELISA: Antibody detection limit dilution 1: 32000.
Immunofluorescence: This antibody shows identical pattern in the Drosophila eye-antennal imaginal disc as was observed before with a different antibody (Moon et al, Mol Cell Biol.2006 Oct;26(20): 7601-15. PMID: 16954388). Recommended concentration: 0.3-1ug/ml.

Immunofluorescence (IF) of anti-E2F1 antibody

(0.5ug/ml) staining of the Drosophila Eye-Antennal Imaginal Disc. Data obtained from Dr. Nam-Sung Moon, McGill University, Montreal, Canada.
Goat Anti-E2F1 Polyclonal Antibody – Cat# MBS422994

Other Notes

Small volumes of anti-E2F1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for E2F1. It may not necessarily be applicable to this product.

NCBI GI #
24648770
NCBI GeneID
42550
NCBI Accession #
NP_524437.2
UniProt Secondary Accession #
O77035; Q5U0Z6
UniProt Related Accession #
Q27368
GenBank Nucleotide #
NM_079713.3
NCBI Official Full Name
E2F transcription factor 1, isoform A
NCBI Official Synonym Full Names
E2F transcription factor 1
NCBI Official Symbol
E2f1
NCBI Official Synonym Symbols
CG6376; dE2f; dE2F; de2f1; dE2f1; dE2F1; def21; DmE2F-1; DmelCG6376; Dp; drosE2F1; DRTF1/E2F; E(Sev-CycE)3A; E(var)3-93E; E(var)3-95E; E(var)93E; e2f; E2f; E2F; E2F-1; e2f1; E2F1; Evar(3)164; l(3)07172; l(3)j3B1; l(3)j3C2; l(3)rM729
UniProt Protein Name
Transcription factor E2f1
UniProt Synonym Protein Names
dE2F
Protein Family
Transcription factor
UniProt Gene Name
E2f1
UniProt Entry Name
E2F1_DROME
NCBI Protein Information
CG6376 gene product from transcript CG6376-RF

UniProt Comments for E2F1

Transcriptional activator that binds to E2f sites. Required for wild-type growth in mitotic and polytene tissues, Contributes to the expression of replication genes at the G1-S transition and Cyclin E. Activates cell proliferation in wing imaginal disk, which requires expression of vg.

Research Articles on E2F1

1. Mechanistically, miR-998 operates by repressing dCbl, a negative regulator of EGFR signaling. Significantly, dCbl is a critical target of miR-998 since dCbl phenocopies the effects of miR-998 on dE2f1-dependent apoptosis in rbf mutants

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-E2F1 antibody

 Reference Product  PubMed Publications
 E2f antibody  >1652 publications with E2F1 and E2f
 E2f2 antibody  >187 publications with E2F1 and E2f2
 Cdc6 antibody  >23 publications with E2F1 and Cdc6
 Rbf antibody  >5 publications with E2F1 and Rbf
 CycE antibody  >4 publications with E2F1 and CycE
 cdc2c antibody  >1 publications with E2F1 and cdc2c
 Rbf2 antibody  >1 publications with E2F1 and Rbf2
 CycA antibody  >1 publications with E2F1 and CycA

Pathways associated with anti-E2F1 antibody

 Products by Pathway  Pathway Diagram
 Assembly Of The Pre-replicative Complex Pathway antibodies  Assembly Of The Pre-replicative Complex Pathway Diagram
 Association Of Licensing Factors With The Pre-replicative Complex Pathway antibodies  Association Of Licensing Factors With The Pre-replicative Complex Pathway Diagram
 CDC6 Association With The ORC:origin Complex Pathway antibodies  CDC6 Association With The ORC:origin Complex Pathway Diagram
 Cell Cycle Pathway antibodies  Cell Cycle Pathway Diagram
 Cell Cycle, Mitotic Pathway antibodies  Cell Cycle, Mitotic Pathway Diagram
 DNA Replication Pathway antibodies  DNA Replication Pathway Diagram
 DNA Replication Pre-Initiation Pathway antibodies  DNA Replication Pre-Initiation Pathway Diagram
 E2F Mediated Regulation Of DNA Replication Pathway antibodies  E2F Mediated Regulation Of DNA Replication Pathway Diagram
 G0 And Early G1 Pathway antibodies  G0 And Early G1 Pathway Diagram
 G1/S Transition Pathway antibodies  G1/S Transition Pathway Diagram

Diseases associated with anti-E2F1 antibody

 Disease Name  Pubmed Publications
 Adenocarcinoma Antibodies  >187 publications with E2F1 and Adenocarcinoma
 Breast Neoplasms Antibodies  >179 publications with E2F1 and Breast Neoplasms
 Neoplasms, Experimental Antibodies  >125 publications with E2F1 and Neoplasms, Experimental
 Lung Neoplasms Antibodies  >120 publications with E2F1 and Lung Neoplasms
 Nervous System Diseases Antibodies  >119 publications with E2F1 and Nervous System Diseases
 Disease Models, Animal Antibodies  >113 publications with E2F1 and Disease Models, Animal
 Liver Neoplasms Antibodies  >106 publications with E2F1 and Liver Neoplasms
 Liver Diseases Antibodies  >103 publications with E2F1 and Liver Diseases
 Carcinoma, Hepatocellular Antibodies  >102 publications with E2F1 and Carcinoma, Hepatocellular
 Melanoma Antibodies  >69 publications with E2F1 and Melanoma

Organs/Tissues associated with anti-E2F1 antibody

 Organ/Tissue Name  Pubmed Publications
 Blood Antibodies  >165 publications with E2F1 and Blood
 Embryonic Tissue Antibodies  >57 publications with E2F1 and Embryonic Tissue
 Head Antibodies  >41 publications with E2F1 and Head
 Gonad Antibodies  >26 publications with E2F1 and Gonad
 Testis Antibodies  >20 publications with E2F1 and Testis
 Ovary Antibodies  >16 publications with E2F1 and Ovary
 Uncharacterized Tissue Antibodies  >4 publications with E2F1 and Uncharacterized Tissue
 Whole Body Antibodies  >2 publications with E2F1 and Whole Body
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