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NCKAP1 Antibody – Goat Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS423012

MBS423012 is an antibody targeting NCKAP1. Also known as Goat anti-NCKAP1 Antibody. Reactive in Expected from sequence similarity: Human, Mouse, Rat, Dog, Pig, Cow. Host: Goat. More details

$450 / 0.1 mg +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
NCKAP1, Polyclonal Antibody
Full Product Name
Goat anti-NCKAP1 Antibody
Product Gene Name
Product Synonym Names
NCKAP1; NCK-associated protein 1; FLJ11291; HEM2; KIAA0587; MGC8981; NAP1; NAP125; NAP 1; OTTHUMP00000163460; OTTHUMP00000163461; membrane-associated protein HEM-2; nck-associated protein 1; p125Nap1; FLJ11291 antibody; HEM2 antibody; KIAA0587 antibody; membrane-associated protein HEM-2 antibody; MGC8981 antibody; NAP 1 antibody; NAP1 antibody; NAP125 antibody; NCKAP1 antibody; NCK-associated protein 1 antibody; OTTHUMP00000163460 antibody; OTTHUMP00000163461 antibody; p125Nap1 antibody
Immunogen Sequence
TPREYLTSHL EIR
Clonality
Polyclonal
Host
Goat
Species Reactivity
Expected from sequence similarity: Human, Mouse, Rat, Dog, Pig, Cow
Form/Format
Supplied at 0.5 mg/ml in Tris saline, 0. 02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Purity/Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Concentration
100ug specific antibody in 200ul (lot specific)
Preparation and Storage
Aliquot and store at -20 degree C. Minimize freezing and thawing.
Antibody/Peptide Pairs
NCKAP1 peptide (MBS427920) is used for blocking the activity of NCKAP1 antibody (MBS423012)
Molecular Weight
129,517 Da
OMIM
604891
Immunogen
Peptide with sequence C-TPREYLTSHLEIR, from the internal region of the protein sequence according to NP_038464.1; NP_995314.1.
Epitope
Internal region
Note
This antibody is expected to recognize both reported isoforms (NP_038464.1; NP_995314.1).
ISO Certification
Manufactured in an ISO 9001:2008 Certified Laboratory.
Applications Tested/Suitable for anti-NCKAP1 antibody
Peptide ELISA (EIA)
Application Notes for anti-NCKAP1 antibody
Peptide ELISA: Antibody detection limit dilution 1: 128000.
Western Blot: Preliminary experiments gave an approx 100kDa band in Human Brain (Cerebellum) lysates after 0.1ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 129kDa according to NP_038464.1. The 100kDa band was successfully blocked by incubation with the immunizing peptide.

Other Notes

Small volumes of anti-NCKAP1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for NCKAP1. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI Related Accession #
UniProt Secondary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
nck-associated protein 1 isoform 1
NCBI Official Synonym Full Names
NCK associated protein 1
NCBI Official Symbol
NCKAP1
NCBI Official Synonym Symbols
HEM2; NAP1; NAP125; p125Nap1
UniProt Protein Name
Nck-associated protein 1
UniProt Synonym Protein Names
Membrane-associated protein HEM-2; p125Nap1
UniProt Gene Name
NCKAP1
UniProt Synonym Gene Names
HEM2; KIAA0587; NAP1; NAP 1
UniProt Entry Name
NCKP1_HUMAN
NCBI Protein Information
nck-associated protein 1

UniProt Comments for NCKAP1

NCKAP1: Part of the WAVE complex that regulates lamellipodia formation. The WAVE complex regulates actin filament reorganization via its interaction with the Arp2/3 complex. Actin remodeling activity is regulated by RAC1. Belongs to the HEM-1/HEM-2 family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral; Apoptosis; Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 2q32

Cellular Component: cytosol; focal adhesion; integral to membrane

Molecular Function: protein binding; protein complex binding; Rac GTPase binding

Biological Process: apoptosis; central nervous system development; Rac protein signal transduction; vascular endothelial growth factor receptor signaling pathway; viral reproduction
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Organs/Tissues associated with anti-NCKAP1 antibody

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