Goat Anti-MCF2L Polyclonal Antibody – Cat# MBS423041

(1ug/ml) staining of Jurkat (A) and Human Thymus (B) lysates (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

MCF2L Antibody – Goat Polyclonal

Name: MCF2L Antibody; Polyclonal; Goat; Human-Reactive; MyBioSource
Brand: MyBioSource
SKU: MBS423041
Price: 450 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS423041

MBS423041 is an antibody targeting MCF2L. Also known as Goat anti-MCF2L Antibody. Reactive in Tested: Human; Expected from sequence similarity: Human, Mouse, Rat, Dog, Cow. Host: Goat. More details

$450 / 0.1 mg +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Online Datasheet in PDF

Product Name

MCF2L, Polyclonal Antibody

Full Product Name

Goat anti-MCF2L Antibody

Product Gene Name

anti-MCF2L antibody

Product Synonym Names

MCF2L; MCF.2 cell line derived transforming sequence-like; ARHGEF14; DBS; FLJ12122; KIAA0362; OST; DBL's big sister; MCF2 transforming sequence-like protein; guanine nucleotide exchange factor DBS; ARHGEF14 antibody; DBL's big sister antibody; DBS antibody; FLJ12122 antibody; guanine nucleotide exchange factor DBS antibody; KIAA0362 antibody; MCF.2 cell line derived transforming sequence-like antibody; MCF2 transforming sequence-like protein antibody; OST antibody; MCF2L antibody

Immunogen Sequence

QLDNQATVQR

Clonality

Polyclonal

Host

Goat

Species Reactivity

Tested: Human; Expected from sequence similarity: Human, Mouse, Rat, Dog, Cow

Form/Format

Supplied at 0.5 mg/ml in Tris saline, 0. 02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

Purity/Purification

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Concentration

100ug specific antibody in 200ul (lot specific)

Preparation and Storage

Aliquot and store at -20 degree C. Minimize freezing and thawing.

Antibody/Peptide Pairs

MCF2L peptide (MBS427912) is used for blocking the activity of MCF2L antibody (MBS423041)

Molecular Weight

126,111 Da

OMIM

609499

Immunogen

Peptide with sequence C-QLDNQATVQR, from the internal region of the protein sequence according to NP_001106203.1; NP_079255.3.

Epitope

Internal region

Note

This antibody is expected to recognize both reported isoforms (NP_001106203.1; NP_079255.3).

ISO Certification

Manufactured in an ISO 9001:2008 Certified Laboratory.

Applications Tested/Suitable for anti-MCF2L antibody

Peptide ELISA (EIA), Western Blot (WB)

Application Notes for anti-MCF2L antibody

Peptide ELISA: Antibody detection limit dilution 1: 128000.
Western Blot: Approx 150kDa band observed in lysates of cell line Jurkat and of Human Thymus (calculated MW of 131kDa according to NP_001106203.1). Recommended concentration: 1-3ug/ml.

Other Notes

Small volumes of anti-MCF2L antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for MCF2L. It may not necessarily be applicable to this product.

NCBI GI #

163644325

NCBI GeneID

23263

NCBI Accession #

NP_001106203.1

NCBI Related Accession #

NP_079255.3

UniProt Secondary Accession #

Q5JU56; Q5VXT1; Q6ZWD4; Q765G8; A2A2X1; A2A2X2; A2A3G6; A2A3G8; B4DHD6; B4DIL6; E9PDN8

UniProt Related Accession #

O15068

GenBank Nucleotide #

NM_001112732.1

NCBI Official Full Name

guanine nucleotide exchange factor DBS isoform a

NCBI Official Synonym Full Names

MCF.2 cell line derived transforming sequence like

NCBI Official Symbol

MCF2L

NCBI Official Synonym Symbols

DBS; OST; ARHGEF14

UniProt Protein Name

Guanine nucleotide exchange factor DBS

UniProt Synonym Protein Names

DBL's big sister; MCF2-transforming sequence-like protein

Protein Family

Guanine nucleotide exchange factor

UniProt Gene Name

MCF2L

UniProt Entry Name

MCF2L_HUMAN

NCBI Protein Information

guanine nucleotide exchange factor DBS

NCBI Summary for MCF2L

This gene encodes a guanine nucleotide exchange factor that interacts specifically with the GTP-bound Rac1 and plays a role in the Rho/Rac signaling pathways. A variant in this gene was associated with osteoarthritis. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Mar 2016]

UniProt Comments for MCF2L

ARHGEF14: Guanine nucleotide exchange factor that potentially links pathways that signal through RAC1, RHOA and CDC42. Catalyzes guanine nucleotide exchange on RHOA and CDC42 and interacts specifically with the GTP-bound form of RAC1, suggesting that it functions as an effector of RAC1. May also participate in axonal transport in the brain. Becomes activated and highly tumorigenic by truncation of the N-terminus. Isoform 5 activates CDC42. Belongs to the MCF2 family. 8 isoforms of the human protein are produced by alternative splicing.

Protein type: Oncoprotein; GEFs; GEFs, Rac/Rho

Chromosomal Location of Human Ortholog: 13q34

Cellular Component: cytoplasm; cytosol; endomembrane system; extracellular space; extrinsic to internal side of plasma membrane; plasma membrane

Molecular Function: guanyl-nucleotide exchange factor activity; phosphoinositide binding; Rho guanyl-nucleotide exchange factor activity

Biological Process: positive regulation of apoptosis; positive regulation of GTPase activity; positive regulation of Rho protein signal transduction; regulation of Rho protein signal transduction; regulation of small GTPase mediated signal transduction

Research Articles on MCF2L

1. MCF2L is subject to a cis-acting eQTL in synovial membrane that correlates with the OA association signal. This signal contains several functional SNPs that could account for the susceptibility and which therefore merit further investigation

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Pathways associated with anti-MCF2L antibody

Products by Pathway	Pathway Diagram
Cell Death Signalling Via NRAGE, NRIF And NADE Pathway antibodies	Cell Death Signalling Via NRAGE, NRIF And NADE Pathway Diagram
G Alpha (12/13) Signalling Events Pathway antibodies	G Alpha (12/13) Signalling Events Pathway Diagram
GPCR Downstream Signaling Pathway antibodies	GPCR Downstream Signaling Pathway Diagram
NRAGE Signals Death Through JNK Pathway antibodies	NRAGE Signals Death Through JNK Pathway Diagram
Neurotrophic Factor-mediated Trk Receptor Signaling Pathway antibodies	Neurotrophic Factor-mediated Trk Receptor Signaling Pathway Diagram
Regulation Of CDC42 Activity Pathway antibodies	Regulation Of CDC42 Activity Pathway Diagram
Regulation Of RhoA Activity Pathway antibodies	Regulation Of RhoA Activity Pathway Diagram
Rho GTPase Cycle Pathway antibodies	Rho GTPase Cycle Pathway Diagram
Signal Transduction Pathway antibodies	Signal Transduction Pathway Diagram
Signaling By GPCR Pathway antibodies	Signaling By GPCR Pathway Diagram

Diseases associated with anti-MCF2L antibody

Disease Name	Pubmed Publications
Cell Transformation, Neoplastic Antibodies	>3 publications with MCF2L and Cell Transformation, Neoplastic
Neurologic Manifestations Antibodies	>1 publications with MCF2L and Neurologic Manifestations
Disease Models, Animal Antibodies	>1 publications with MCF2L and Disease Models, Animal
Nervous System Diseases Antibodies	>1 publications with MCF2L and Nervous System Diseases
Liver Diseases Antibodies	>1 publications with MCF2L and Liver Diseases
Neurobehavioral Manifestations Antibodies	>1 publications with MCF2L and Neurobehavioral Manifestations
Hypertrophy Antibodies	>1 publications with MCF2L and Hypertrophy
Kidney Diseases Antibodies	>1 publications with MCF2L and Kidney Diseases

Organs/Tissues associated with anti-MCF2L antibody

Organ/Tissue Name	Pubmed Publications
Brain Antibodies	>4 publications with MCF2L and Brain
Thymus Antibodies	>2 publications with MCF2L and Thymus
Prostate Antibodies	>2 publications with MCF2L and Prostate
Muscle Antibodies	>2 publications with MCF2L and Muscle
Ascites Antibodies	>1 publications with MCF2L and Ascites
Heart Antibodies	>1 publications with MCF2L and Heart
Liver Antibodies	>1 publications with MCF2L and Liver
Intestine Antibodies	>1 publications with MCF2L and Intestine
Kidney Antibodies	>1 publications with MCF2L and Kidney
Parathyroid Antibodies	>1 publications with MCF2L and Parathyroid