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C-FOS (FOS) Antibody – Goat

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS423052

MBS423052 is an antibody targeting FOS. Also known as Goat anti-c-FOS (aa283-295) Antibody. Reactive in Tested: Human; Expected from sequence similarity: Human, Dog, Pig, Cow. Host: Goat. More details

$450 / 0.1 mg +1 FREE 8GB USB
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Product Overview

Online Datasheet in PDF
Product Name
c-FOS, Antibody
Full Product Name
Goat anti-c-FOS (aa283-295) Antibody
Product Gene Name
anti-FOS antibody
Product Synonym Gene Name
c-FOS
Product Synonym Names
activator protein 1, AP-1, cellular oncogene c-fos, C-FOS, FBJ murine osteosarcoma viral (v-fos) oncogene homolog (oncogene FOS), FBJ murine osteosarcoma viral oncogene homolog, FOS, G0/G1 switch regulatory protein 7, p55, proto-oncogene c-Fos
Host
Goat
Species Reactivity
Tested: Human; Expected from sequence similarity: Human, Dog, Pig, Cow
Form/Format
Supplied at 0.5 mg/ml in Tris saline, 0. 02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Purity/Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Preparation and Storage
Aliquot and store at -20 degrees C. Minimize freezing and thawing.
Antibody/Peptide Pairs
c-FOS peptide (MBS427916) is used for blocking the activity of c-FOS antibody (MBS423052)
OMIM
164810
Immunogen
Peptide with sequence C-SETARSVPDMDLS, from the internal region of the protein sequence according to NP_005243.1.
ISO Certification
Manufactured in an ISO 9001:2008 Certified Laboratory.
Applications Tested/Suitable for anti-FOS antibody
Peptide ELISA (EIA), Western Blot (WB), Immunofluroescence (IF), Immunohistochemistry (IHC)
Application Notes for anti-FOS antibody
Peptide ELISA: Antibody detection limit dilution 1: 128000.
IHC: Paraffin embedded Human Tonsil. Recommended concentration: 8ug/ml
Immunofluorescence : Strong expression of the protein seen in the nucleusof HeLa cells, and in the ER and cytoplasm of MCF7 cells. Recommended concentration : 10ug/mL.

Western Blot (WB) of anti-FOS antibody

MBS423052(0.3ug/ml) staining of HeLa lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Goat Anti-FOS Antibody – Cat# MBS423052

Immunohistochemistry (IHC) of anti-FOS antibody

MBS423052 (5ug/ml) staining of paraffin embedded Human Tonsil. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
Goat Anti-FOS Antibody, Immunohistochemistry (IHC) – Cat# MBS423052

Immunofluorescence (IF) of anti-FOS antibody

Immunofluorescence analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing strong nuclear and weak cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml)
Goat Anti-FOS Antibody, Immunofluorescence (IF) – Cat# MBS423052

Immunofluorescence (IF) of anti-FOS antibody

Immunofluorescence analysis of paraformaldehyde fixed MCF7 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing endoplasmic reticulum and cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).
Goat Anti-FOS Antibody, Immunofluorescence (IF) – Cat# MBS423052

Immunohistochemistry (IHC) of anti-FOS antibody

(8ug/ml) staining of paraffin embedded Human Tonsil. Heat induced antigen retrieval with citrate buffer pH 6, HRP-staining.
Goat Anti-FOS Antibody, Immunohistochemistry (IHC) – Cat# MBS423052

Immunohistochemistry (IHC) of anti-FOS antibody

Negative Control showing staining of paraffin embedded Human Tonsil, with no primary antibody.
Goat Anti-FOS Antibody, Immunohistochemistry (IHC) – Cat# MBS423052

Other Notes

Small volumes of anti-FOS antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for FOS. It may not necessarily be applicable to this product.

NCBI GI #
4885241
NCBI GeneID
2353
NCBI Accession #
NP_005243.1
UniProt Secondary Accession #
P18849; A8K4E2; B4DQ65
UniProt Related Accession #
P01100
GenBank Nucleotide #
NM_005252.3
NCBI Official Full Name
proto-oncogene c-Fos
NCBI Official Synonym Full Names
Fos proto-oncogene, AP-1 trancription factor subunit
NCBI Official Symbol
FOS
NCBI Official Synonym Symbols
p55; AP-1; C-FOS
UniProt Protein Name
Proto-oncogene c-Fos
UniProt Synonym Protein Names
Cellular oncogene fos; G0/G1 switch regulatory protein 7
Protein Family
Proto-oncogene
UniProt Gene Name
FOS
UniProt Synonym Gene Names
G0S7
UniProt Entry Name
FOS_HUMAN
NCBI Protein Information
proto-oncogene c-Fos

NCBI Summary for FOS

The Fos gene family consists of 4 members: FOS, FOSB, FOSL1, and FOSL2. These genes encode leucine zipper proteins that can dimerize with proteins of the JUN family, thereby forming the transcription factor complex AP-1. As such, the FOS proteins have been implicated as regulators of cell proliferation, differentiation, and transformation. In some cases, expression of the FOS gene has also been associated with apoptotic cell death. [provided by RefSeq, Jul 2008]
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UniProt Comments for FOS

Fos: a proto-oncogenic transcription factor of the bZIP family. Dimerizes with proteins of the JUN family, thereby forming the transcription factor complex AP-1. FOS proteins function as regulators of cell proliferation, differentiation, and transformation. In some cases, expression of FOS has also been associated with apoptotic cell death. Expression increases upon a variety of stimuli, including growth factors, cytokines, neurotransmitters, polypeptide hormones, stress and cell injury.

Protein type: DNA-binding; Motility/polarity/chemotaxis; Oncoprotein; Transcription factor

Chromosomal Location of Human Ortholog: 14q24.3

Cellular Component: nucleoplasm; nucleus

Molecular Function: protein binding; transcription factor activity; transcription factor binding

Biological Process: DNA methylation; inflammatory response; positive regulation of transcription from RNA polymerase II promoter; positive regulation of transcription, DNA-dependent; regulation of transcription factor activity; regulation of transcription from RNA polymerase II promoter; transforming growth factor beta receptor signaling pathway
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Research Articles on FOS

1. The translocation causes truncation of the FOS protein, with loss of the transactivation domain, which is thereby a novel mechanism involved in tumorigenesis.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-FOS antibody

 Reference Product  PubMed Publications
 JUN antibody  >8466 publications with FOS and JUN
 JUNB antibody  >733 publications with FOS and JUNB
 JUND antibody  >516 publications with FOS and JUND
 MAPK1 antibody  >470 publications with FOS and MAPK1
 SRF antibody  >283 publications with FOS and SRF
 RHOA antibody  >76 publications with FOS and RHOA
 CREB1 antibody  >65 publications with FOS and CREB1
 MAPK8 antibody  >49 publications with FOS and MAPK8
 SMAD3 antibody  >40 publications with FOS and SMAD3
 NFATC2 antibody  >21 publications with FOS and NFATC2

Pathways associated with anti-FOS antibody

 Products by Pathway  Pathway Diagram
 ATF-2 Transcription Factor Network Pathway antibodies  ATF-2 Transcription Factor Network Pathway Diagram
 Activated TLR4 Signalling Pathway antibodies  Activated TLR4 Signalling Pathway Diagram
 Activation Of The AP-1 Family Of Transcription Factors Pathway antibodies  Activation Of The AP-1 Family Of Transcription Factors Pathway Diagram
 Amphetamine Addiction Pathway antibodies  Amphetamine Addiction Pathway Diagram
 Amphetamine Addiction Pathway antibodies  Amphetamine Addiction Pathway Diagram
 Apoptosis Pathway antibodies  Apoptosis Pathway Diagram
 Apoptosis Pathway antibodies  Apoptosis Pathway Diagram
 Apoptosis Modulation And Signaling Pathway antibodies  Apoptosis Modulation And Signaling Pathway Diagram
 B Cell Receptor Signaling Pathway antibodies  B Cell Receptor Signaling Pathway Diagram
 B Cell Receptor Signaling Pathway antibodies  B Cell Receptor Signaling Pathway Diagram

Diseases associated with anti-FOS antibody

 Disease Name  Pubmed Publications
 Nervous System Diseases Antibodies  >3040 publications with FOS and Nervous System Diseases
 Pain Antibodies  >1535 publications with FOS and Pain
 Inflammation Antibodies  >1098 publications with FOS and Inflammation
 Necrosis Antibodies  >779 publications with FOS and Necrosis
 Epilepsy Antibodies  >542 publications with FOS and Epilepsy
 Seizures Antibodies  >524 publications with FOS and Seizures
 Hyperalgesia Antibodies  >438 publications with FOS and Hyperalgesia
 Breast Neoplasms Antibodies  >387 publications with FOS and Breast Neoplasms
 Hypertension Antibodies  >386 publications with FOS and Hypertension
 Heart Diseases Antibodies  >374 publications with FOS and Heart Diseases

Organs/Tissues associated with anti-FOS antibody

 Organ/Tissue Name  Pubmed Publications
 Brain Antibodies  >11626 publications with FOS and Brain
 Blood Antibodies  >3784 publications with FOS and Blood
 Nerve Antibodies  >2696 publications with FOS and Nerve
 Muscle Antibodies  >1518 publications with FOS and Muscle
 Spinal Cord Antibodies  >1515 publications with FOS and Spinal Cord
 Bone Antibodies  >1353 publications with FOS and Bone
 Vascular Antibodies  >1207 publications with FOS and Vascular
 Liver Antibodies  >1030 publications with FOS and Liver
 Heart Antibodies  >990 publications with FOS and Heart
 Skin Antibodies  >723 publications with FOS and Skin
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