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POU6F1 Antibody – Goat Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS423089

MBS423089 is an antibody targeting POU6F1. Also known as Goat anti-POU6F1 Antibody. Reactive in Tested: Human, Mouse; Expected from sequence similarity: Human, Mouse, Rat, Dog, Cow. Host: Goat. More details

$450 / 0.1 mg +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
POU6F1, Polyclonal Antibody
Full Product Name
Goat anti-POU6F1 Antibody
Product Gene Name
Product Synonym Names
POU6F1; POU class 6 homeobox 1; BRN5; MPOU; TCFB1; POU domain, class 6, transcription factor 1; BRN5 antibody; MPOU antibody; POU class 6 homeobox 1 antibody; POU domain; class 6; transcription factor 1 antibody; TCFB1 antibody; POU6F1 antibody
Immunogen Sequence
ETPTVSQLVS KPHTP
Clonality
Polyclonal
Host
Goat
Species Reactivity
Tested: Human, Mouse; Expected from sequence similarity: Human, Mouse, Rat, Dog, Cow
Form/Format
Supplied at 0.5 mg/ml in Tris saline, 0. 02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Purity/Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Concentration
100ug specific antibody in 200ul (lot specific)
Preparation and Storage
Aliquot and store at -20 degree C. Minimize freezing and thawing.
Antibody/Peptide Pairs
POU6F1 peptide (MBS427839) is used for blocking the activity of POU6F1 antibody (MBS423089)
Molecular Weight
32,645 Da
Immunogen
Peptide with sequence C-ETPTVSQLVSKPHTP, from the internal region of the protein sequence according to NP_002693.3.
Epitope
Internal region
ISO Certification
Manufactured in an ISO 9001:2008 Certified Laboratory.
Applications Tested/Suitable for anti-POU6F1 antibody
Peptide ELISA (EIA), Western Blot (WB)
Application Notes for anti-POU6F1 antibody
Peptide ELISA: Antibody detection limit dilution 1: 128000.
Western Blot: Approx 30kDa band observed in Human Brain (Cerebral cortex) and Mouse fetal Brain lysates (calculated MW of 32.6kDa according to NP_002693.3). Recommended concentration: 0.3-1ug/ml.

Western Blot (WB) of anti-POU6F1 antibody

(0.3ug/ml) staining of Human Cerebral Cortex lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Goat Anti-POU6F1 Polyclonal Antibody – Cat# MBS423089

Western Blot (WB) of anti-POU6F1 antibody

(0. 05ug/ml) staining of Mouse fetal Brain lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Goat Anti-POU6F1 Polyclonal Antibody, Western Blot (WB) – Cat# MBS423089

Other Notes

Small volumes of anti-POU6F1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for POU6F1. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Secondary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
POU domain, class 6, transcription factor 1
NCBI Official Synonym Full Names
POU class 6 homeobox 1
NCBI Official Symbol
POU6F1
NCBI Official Synonym Symbols
BRN5; MPOU; TCFB1
UniProt Protein Name
POU domain, class 6, transcription factor 1
UniProt Synonym Protein Names
Brain-specific homeobox/POU domain protein 5; Brain-5; Brn-5; mPOU homeobox protein
UniProt Gene Name
POU6F1
UniProt Synonym Gene Names
BRN5; MPOU; TCFB1; Brain-5; Brn-5
UniProt Entry Name
PO6F1_HUMAN
NCBI Protein Information
POU domain, class 6, transcription factor 1

UniProt Comments for POU6F1

POU6F1: Transcription factor that binds preferentially to a variant of the octamer motif (5'-ATGATAAT-3'). Belongs to the POU transcription factor family. Class- 6 subfamily.

Protein type: DNA-binding; Transcription factor

Chromosomal Location of Human Ortholog: 12q13.13

Cellular Component: actin cytoskeleton; nucleus

Molecular Function: transcription factor activity

Biological Process: brain development; heart development; muscle development; regulation of transcription, DNA-dependent; transcription, DNA-dependent
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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