Goat Anti-HSP31P Polyclonal Antibody – Cat# MBS423090

(0. 05ug/ml) staining of yeast lysate (wt) and the KO (delta) in th eleft panel and the Ponceau stain in the right panel (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence. Data obtained by Dr. M. Skoneczny, IBB, Warsaw, Poland.

HSP31P Antibody – Goat Polyclonal

Name: HSP31P Antibody; Polyclonal; Goat; Saccharomyces-Reactive; MyBioSource
Brand: MyBioSource
SKU: MBS423090
Price: 450 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS423090

MBS423090 is an antibody targeting Hsp31p. Also known as Goat anti-Hsp31p (yeast, aa50-64) Antibody. Reactive in Tested: S. cerevisiae; Expected from sequence similarity: Saccharomyces cerevisiae S288c. Host: Goat. More details

$450 / 0.1 mg +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Online Datasheet in PDF

Product Name

Hsp31p, Polyclonal Antibody

Full Product Name

Goat anti-Hsp31p (yeast, aa50-64) Antibody

Product Gene Name

anti-Hsp31p antibody

Product Synonym Names

Hsp31p; YDR533C; Hsp31p antibody; YDR533C antibody; Hsp31p (yeast, aa50-64)

Immunogen Sequence

ETGKFGWDEH SLAKD

Clonality

Polyclonal

Host

Goat

Species Reactivity

Tested: S. cerevisiae; Expected from sequence similarity: Saccharomyces cerevisiae S288c

Form/Format

Supplied at 0.5 mg/ml in Tris saline, 0. 02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

Purity/Purification

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Concentration

100ug specific antibody in 200ul (lot specific)

Preparation and Storage

Aliquot and store at -20 degree C. Minimize freezing and thawing.

Antibody/Peptide Pairs

Hsp31p peptide (MBS427974) is used for blocking the activity of Hsp31p antibody (MBS423090)

Molecular Weight

25,670 Da

Immunogen

Peptide with sequence C-ETGKFGWDEHSLAKD, from the internal region of the protein sequence according to NP_010822.1.

Epitope

Internal region

ISO Certification

Manufactured in an ISO 9001:2008 Certified Laboratory.

Applications Tested/Suitable for anti-Hsp31p antibody

Peptide ELISA (EIA), Western Blot (WB)

Application Notes for anti-Hsp31p antibody

Peptide ELISA: Antibody detection limit dilution 1: 128000.
Western Blot: Approx 24kDa band observed in lysates of postdiauxic (overnight grown yeast cells, and no signal in KO grown under identical conditions. Cells grown in logarithmic phase show diminished signals as expected. (calculated MW of 25.7kDa according to NP_010822.1). Recommended concentration: 0. 02-0. 06ug/ml.

Other Notes

Small volumes of anti-Hsp31p antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for Hsp31p. It may not necessarily be applicable to this product.

NCBI GI #

6320742

NCBI GeneID

852146

NCBI Accession #

NP_010822.1

UniProt Secondary Accession #

D6VTF4; E9P8V9

UniProt Related Accession #

Q04432

GenBank Nucleotide #

NM_001180841.1

NCBI Official Full Name

glutathione-independent methylglyoxalase

NCBI Official Symbol

HSP31

UniProt Protein Name

Glutathione-independent glyoxalase HSP31

UniProt Synonym Protein Names

Glyoxalase 3 homolog 1

UniProt Gene Name

HSP31

UniProt Entry Name

HSP31_YEAST

NCBI Protein Information

glutathione-independent methylglyoxalase

UniProt Comments for Hsp31p

Catalyzes the conversion of methylglyoxal (MG) to D-lactate in a single glutathione (GSH)-independent step. May play a role in detoxifying endogenously produced glyoxals (PubMed:24302734). Involved in protection against reactive oxygen species (ROS) (PubMed:17395014). Important for viability in stationary phase. May negatively regulate TORC1 in response to nutrient limitation (PubMed:24706893).

Research Articles on Hsp31p

1. Hsp31 maintains cellular glutathione and NADPH levels, thus conferring protection against oxidative stress

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.