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PDLIM2 Antibody – Goat Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS423131

MBS423131 is an antibody targeting PDLIM2. Also known as Goat anti-PDLIM2 Antibody. Reactive in Tested: Mouse; Expected from sequence similarity: Human, Mouse, Rat, Pig, Cow. Host: Goat. More details

$450 / 0.1 mg +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Product Overview

Online Datasheet in PDF
Product Name
PDLIM2, Polyclonal Antibody
Full Product Name
Goat anti-PDLIM2 Antibody
Product Gene Name
Product Synonym Names
PDLIM2; PDZ and LIM domain 2 (mystique); MYSTIQUE; SLIM; PDZ and LIM domain protein 2; PDLIM2 antibody; PDZ and LIM domain 2 (mystique) antibody; MYSTIQUE antibody; SLIM antibody; PDZ and LIM domain protein 2 antibody; PDLIM2
Immunogen Sequence
NQAVRIQEGR YRHP
Clonality
Polyclonal
Host
Goat
Species Reactivity
Tested: Mouse; Expected from sequence similarity: Human, Mouse, Rat, Pig, Cow
Form/Format
Supplied at 0.5 mg/ml in Tris saline, 0. 02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Purity/Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Concentration
100ug specific antibody in 200ul (lot specific)
Preparation and Storage
Aliquot and store at -20 degree C. Minimize freezing and thawing.
Antibody/Peptide Pairs
PDLIM2 peptide (MBS427788) is used for blocking the activity of PDLIM2 antibody (MBS423131)
Molecular Weight
62,746 Da
OMIM
609722
Immunogen
Peptide with sequence C-NQAVRIQEGRYRHP, from the internal region of the protein sequence according to NP_789847.1; NP_067643.3; NP_932159.1.
Epitope
Internal region
Note
This antibody is expected to recognize reported isoforms 1 and 2 (NP_789847.1; NP_067643.3).
ISO Certification
Manufactured in an ISO 9001:2008 Certified Laboratory.
Applications Tested/Suitable for anti-PDLIM2 antibody
Peptide ELISA (EIA), Western Blot (WB)
Application Notes for anti-PDLIM2 antibody
Peptide ELISA: Antibody detection limit dilution 1: 128000.
Western Blot: Approx 38kDa bands observed in Mouse Lung lysates (calculated MW of 37.7kDa according to Mouse NP_666090.1). Recommended concentration: 0.3-1ug/ml.

Western Blot (WB) of anti-PDLIM2 antibody

(0.3ug/ml) staining of Mouse Lung lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Goat Anti-PDLIM2 Polyclonal Antibody – Cat# MBS423131

Other Notes

Small volumes of anti-PDLIM2 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for PDLIM2. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI Related Accession #
UniProt Secondary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
PDZ and LIM domain protein 2 isoform 1
NCBI Official Synonym Full Names
PDZ and LIM domain 2
NCBI Official Symbol
PDLIM2
NCBI Official Synonym Symbols
SLIM; MYSTIQUE
UniProt Protein Name
PDZ and LIM domain protein 2
UniProt Synonym Protein Names
PDZ-LIM protein mystique
UniProt Gene Name
PDLIM2
UniProt Entry Name
PDLI2_HUMAN
NCBI Protein Information
PDZ and LIM domain protein 2

NCBI Summary for PDLIM2

This gene encodes a member of the ALP subfamily of PDZ-LIM domain proteins. The encoded protein suppresses anchorage-dependent growth and promotes cell migration and adhesion through interactions with the actin cytoskeleton via the PDZ domain. The encoded protein is also a putative tumor suppressor protein, and decreased expression of this gene is associated with several malignancies including breast cancer and adult T-cell leukemia. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Oct 2011]
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UniProt Comments for PDLIM2

PDLIM2: Probable adapter protein located at the actin cytoskeleton that promotes cell attachment. Necessary for the migratory capacity of epithelial cells. Overexpression enhances cell adhesion to collagen and fibronectin and suppresses anchorage independent growth. May contribute to tumor cell migratory capacity. Interacts with alpha-actinins ACTN1 and ACTN4, FLNA and MYH9. 4 isoforms of the human protein are produced by alternative splicing.

Protein type: Ubiquitin ligase; Cell adhesion

Chromosomal Location of Human Ortholog: 8p21.3

Cellular Component: actin cytoskeleton; cortical actin cytoskeleton; focal adhesion; nucleus; stress fiber

Molecular Function: filamin binding; muscle alpha-actinin binding; myosin heavy chain binding; protein binding; zinc ion binding
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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