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NSG1 Antibody – Goat Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS423142

MBS423142 is an antibody targeting Nsg1. Also known as Goat anti-Nsg1/Neep21 Antibody. Reactive in Tested: Mouse, Rat; Expected from sequence similarity: Human, Mouse, Rat, Cow. Host: Goat. More details

$450 / 0.1 mg +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
Nsg1/Neep21, Polyclonal Antibody
Full Product Name
Goat anti-Nsg1/Neep21 Antibody
Product Gene Name
Product Synonym Gene Name
Neep21
Product Synonym Names
Nsg1; neuron specific gene family member 1; Bsmrb; Neep21; PEP1; brain neuron cytoplasmic protein 1/2; brain specific mRNA b; neuron-specific protein family member 1; brain neuron cytoplasmic protein 1/2 antibody; brain specific mRNA b antibody; Bsmrb antibody; Neep21 antibody; neuron specific gene family member 1 antibody; neuron-specific protein family member 1 antibody; Nsg1 antibody; PEP1 antibody; Nsg1 / Neep21
Immunogen Sequence
EKGTKQPLLE DGFD
Clonality
Polyclonal
Host
Goat
Species Reactivity
Tested: Mouse, Rat; Expected from sequence similarity: Human, Mouse, Rat, Cow
Form/Format
Supplied at 0.5 mg/ml in Tris saline, 0. 02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Purity/Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Concentration
100ug specific antibody in 200ul (lot specific)
Preparation and Storage
Aliquot and store at -20 degree C. Minimize freezing and thawing.
Antibody/Peptide Pairs
Molecular Weight
20,943 Da
Immunogen
Peptide with sequence EKGTKQPLLEDGFD-C, from the N Terminus of the protein sequence according to NP_077042.2.
Epitope
N Terminus
ISO Certification
Manufactured in an ISO 9001:2008 Certified Laboratory.
Applications Tested/Suitable for anti-Nsg1 antibody
Peptide ELISA (EIA), Western Blot (WB), Immunocytochemistry (ICC)
Application Notes for anti-Nsg1 antibody
Peptide ELISA: Antibody detection limit dilution 1: 128000.
Immunohistochemistry: Immunocytochemistry: Primary DIV9 cells from Hippocampus E18 Rat embryos, stained for neuronal marker MAP2 in blue and for glial marker GFAP in green showed exclusive labelling of this product (red) in the neurons only. Recommended concentration, 1-5ug/ml.
Western Blot: Approx 22kDa band observed in Mouse fetal Brain lysates (calculated MW of 20.9kDa according to NP_035072.2). Recommended concentration: 0.1-0.3ug/ml.
Immunocytochemistry: Primary DIV9 cells from Hippocampus E18 Rat embryos, stained for neuronal marker MAP2 in blue and for glial marker GFAP in green showed exclusive labelling of (red) in the neurons only. Recommended concentration, 1-5ug/ml.

Western Blot (WB) of anti-Nsg1 antibody

(0.1ug/ml) staining of fetal Mouse Brain lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Goat Anti-NSG1 Polyclonal Antibody – Cat# MBS423142

Other Notes

Small volumes of anti-Nsg1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for Nsg1. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Secondary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
neuron-specific protein family member 1
NCBI Official Synonym Full Names
neuron specific gene family member 1
NCBI Official Symbol
Nsg1
NCBI Official Synonym Symbols
PEP1; Bsmrb; Neep21
UniProt Protein Name
Neuron-specific protein family member 1
UniProt Synonym Protein Names
Brain neuron cytoplasmic protein 1/2
UniProt Gene Name
Nsg1
UniProt Synonym Gene Names
Bsmrb
UniProt Entry Name
NSG1_RAT
NCBI Protein Information
neuron-specific protein family member 1

NCBI Summary for Nsg1

(brain-specific protein that) may be involved in neuronal fiber networks [RGD, Feb 2006]

UniProt Comments for Nsg1

D4S234E: Belongs to the NSG family.

Protein type: Membrane protein, integral

Cellular Component: endosome; Golgi membrane; integral to membrane; lateral plasma membrane; postsynaptic membrane

Molecular Function: clathrin light chain binding; receptor binding

Biological Process: clathrin cage assembly; dopamine receptor signaling pathway; endosome transport; positive regulation of receptor recycling
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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