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MEX3C Antibody – Goat Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS423144

MBS423144 is an antibody targeting MEX3C. Also known as Goat anti-MEX3C (aa541-554) Antibody. Reactive in Tested: Mouse; Expected from sequence similarity: Human, Mouse, Rat, Dog, Pig, Cow. Host: Goat. More details

$450 / 0.1 mg +1 FREE 8GB USB
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
MEX3C, Polyclonal Antibody
Full Product Name
Goat anti-MEX3C (aa541-554) Antibody
Product Gene Name
Product Synonym Names
MEX3C; mex-3 homolog C (C. elegans); FLJ38871; MEX-3C; RKHD2; RNF194; OTTHUMP00000163545; RING finger and KH domain-containing protein 2; RING finger protein 194; RNA-binding protein MEX3C; ring finger and KH domain containing 2; FLJ38871 antibody; mex-3 homolog C (C. elegans) antibody; MEX3C antibody; MEX-3C antibody; OTTHUMP00000163545 antibody; ring finger and KH domain containing 2 antibody; RING finger and KH domain-containing protein 2 antibody; RING finger protein 194 antibody; RKHD2 antibody; RNA-binding protein MEX3C antibody; RNF194 antibody; MEX3C (aa541-554)
Immunogen Sequence
TPRLSPTFPE SIEH
Clonality
Polyclonal
Host
Goat
Species Reactivity
Tested: Mouse; Expected from sequence similarity: Human, Mouse, Rat, Dog, Pig, Cow
Form/Format
Supplied at 0.5 mg/ml in Tris saline, 0. 02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Purity/Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Concentration
100ug specific antibody in 200ul (lot specific)
Preparation and Storage
Aliquot and store at -20 degree C. Minimize freezing and thawing.
Antibody/Peptide Pairs
MEX3C peptide (MBS427984) is used for blocking the activity of MEX3C antibody (MBS423144)
Molecular Weight
69,366 Da
OMIM
611005
Immunogen
Peptide with sequence C-TPRLSPTFPESIEH, from the internal region of the protein sequence according to NP_057710.3.
Epitope
Internal region
ISO Certification
Manufactured in an ISO 9001:2008 Certified Laboratory.
Applications Tested/Suitable for anti-MEX3C antibody
Peptide ELISA (EIA), Western Blot (WB)
Application Notes for anti-MEX3C antibody
Peptide ELISA: Antibody detection limit dilution 1: 32000.
Western Blot: Approx 60+36kDa bands observed in Mouse Testis lysates, and these bands reduced after knock-down by gene trap (calculated MW of 69.4kDa according to NP_057710.3). Recommended concentration: 0.2-0.5ug/ml.

Western Blot (WB) of anti-MEX3C antibody

(0.2ug/ml) staining of Mouse Testis (+/+ is wt, trp/trp is knock-down) lysate (35ug protein in RIPA buffer). Data obtained by Dr. B. Lu, Wake Forest Baptist Medical Center, Winston-Salem, NC, USA. Primary incubation was 1 hour. Detected by chemiluminescence.
Goat Anti-MEX3C Polyclonal Antibody – Cat# MBS423144

Other Notes

Small volumes of anti-MEX3C antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for MEX3C. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Secondary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
RNA-binding E3 ubiquitin-protein ligase MEX3C
NCBI Official Synonym Full Names
mex-3 RNA binding family member C
NCBI Official Symbol
MEX3C
NCBI Official Synonym Symbols
RKHD2; BM-013; MEX-3C; RNF194
UniProt Protein Name
RNA-binding E3 ubiquitin-protein ligase MEX3C
UniProt Synonym Protein Names
RING finger and KH domain-containing protein 2; RING finger protein 194
UniProt Gene Name
MEX3C
UniProt Synonym Gene Names
RKHD2; RNF194
UniProt Entry Name
MEX3C_HUMAN
NCBI Protein Information
RNA-binding E3 ubiquitin-protein ligase MEX3C

NCBI Summary for MEX3C

This gene encodes a member of a family of proteins with two K homology (KH) RNA-binding domains and a C-terminal RING-finger domain. The protein interacts with mRNA via the KH domains, and the protein shuttles between the nucleus and cytoplasm. Polymorphisms in this gene may contribute to hypertension. [provided by RefSeq, Oct 2009]
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UniProt Comments for MEX3C

RKHD2: E3 ubiquitin ligase responsible for the post- transcriptional regulation of common HLA-A allotypes. Binds to the 3' UTR of HLA-A2 mRNA, and regulates its levels by promoting mRNA decay. RNA binding is sufficient to prevent translation, but ubiquitin ligase activity is required for mRNA degradation. Genetic variations in MEX3C may be associated with susceptibility to essential hypertension.

Protein type: Ubiquitin conjugating system; RNA-binding

Chromosomal Location of Human Ortholog: 18q21.2

Cellular Component: cytoplasm; nucleus

Molecular Function: ligase activity; protein binding; zinc ion binding

Biological Process: regulation of fat cell differentiation

Disease: Hypertension, Essential, Susceptibility To, 8
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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