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RRAD Antibody – Goat Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS423159

MBS423159 is an antibody targeting RRAD. Also known as Goat anti-RRAD (aa36-48) Antibody. Reactive in Tested: Mouse; Expected from sequence similarity: Human, Mouse, Rat, Dog, Pig, Cow. Host: Goat. More details

$450 / 0.1 mg +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
RRAD, Polyclonal Antibody
Full Product Name
Goat anti-RRAD (aa36-48) Antibody
Product Gene Name
anti-RRAD antibody
Product Synonym Names
RRAD; Ras-related associated with diabetes; RAD; RAD1; REM3; GTP-binding protein RAD; OTTHUMP00000174805; RAS (RAD and GEM) like GTP binding 3; ras associated with diabetes; GTP-binding protein RAD antibody; OTTHUMP00000174805 antibody; RAD antibody; RAD1 antibody; RAS (RAD and GEM) like GTP binding 3 antibody; ras associated with diabetes antibody; Ras-related associated with diabetes antibody; REM3 antibody; RRAD antibody; RRAD (aa36-48)
Immunogen Sequence
HRRSMPVDER DLQ
Clonality
Polyclonal
Host
Goat
Species Reactivity
Tested: Mouse; Expected from sequence similarity: Human, Mouse, Rat, Dog, Pig, Cow
Form/Format
Supplied at 0.5 mg/ml in Tris saline, 0. 02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Purity/Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Concentration
100ug specific antibody in 200ul (lot specific)
Preparation and Storage
Aliquot and store at -20 degree C. Minimize freezing and thawing.
Antibody/Peptide Pairs
RRAD peptide (MBS427795) is used for blocking the activity of RRAD antibody (MBS423159)
Molecular Weight
33,245 Da
OMIM
179503
Immunogen
Peptide with sequence C-HRRSMPVDERDLQ, from the internal region of the protein sequence according to NP_004156.1.
Epitope
Internal region
Note
Reported variants represent identical protein: NP_004156.1, NP_001122322.1
ISO Certification
Manufactured in an ISO 9001:2008 Certified Laboratory.
Applications Tested/Suitable for anti-RRAD antibody
Peptide ELISA (EIA), Western Blot (WB)
Application Notes for anti-RRAD antibody
Peptide ELISA: Antibody detection limit dilution 1: 64000.
Western Blot: Approx 37kDa band observed in Mouse Heart lysates and no 37kDa in the KO lysates (calculated MW of 33.2kDa according to mouse NP_062636.2 and human NP_004156.1). There is some non-specific background, we call for caution when used for other assays than Western blot. In transfected HEK293 transiently expressing Mouse Rrad (HA tagged), a band of approx. 45kDa is observed. No bands are observed in transfected HEK293 transiently expressing related Mouse GTPases. Recommended concentration: 0.5-2ug/ml.

Western Blot (WB) of anti-RRAD antibody

A): (0.5ug/ml) staining of HEK293 lysates overexpressing several HA-tagged Mouse GTPases, including Rrad (10ug protein in RIPA buffer) and compared with an HA-specific antibody. B): (0.5ug/ml) staining of WT and KO lysates of Mouse Heart (100ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence. Data provided by Prof. D Andres, University of Kentucky College of Medicine, USA.
Goat Anti-RRAD Polyclonal Antibody – Cat# MBS423159

Other Notes

Small volumes of anti-RRAD antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for RRAD. It may not necessarily be applicable to this product.

NCBI GI #
4759054
NCBI GeneID
6236
NCBI Accession #
NP_004156.1
UniProt Secondary Accession #
Q96F39
UniProt Related Accession #
P55042
GenBank Nucleotide #
NM_004165.2
NCBI Official Full Name
GTP-binding protein RAD
NCBI Official Synonym Full Names
RRAD, Ras related glycolysis inhibitor and calcium channel regulator
NCBI Official Symbol
RRAD
NCBI Official Synonym Symbols
RAD; RAD1; REM3
UniProt Protein Name
GTP-binding protein RAD
UniProt Synonym Protein Names
RAD1; Ras associated with diabetes
Protein Family
GTP-binding protein
UniProt Gene Name
RRAD
UniProt Synonym Gene Names
RAD
UniProt Entry Name
RAD_HUMAN
NCBI Protein Information
GTP-binding protein RAD

UniProt Comments for RRAD

RRAD: a small G protein of the RGK family. May play an important role in cardiac antiarrhythmia via the strong suppression of voltage-gated L-type Ca(2+) currents. Regulates voltage-dependent L-type calcium channel subunit alpha- 1C trafficking to the cell membrane. Inhibits cardiac hypertrophy through the calmodulin-dependent kinase II (CaMKII) pathway. Interacts with calmodulin preferentially in the inactive, GDP-bound form. Interacts with CAMK2D and CACNB2. Interaction with CACNB2 regulates the trafficking of CACNA1C to the cell membrane. Most abundantly expressed in the heart. Also found in the skeletal muscle and lung. Lesser amounts in placenta and kidney. Also detected in adipose tissue. Overexpressed in muscle of type II diabetic humans.

Protein type: G protein, monomeric, RGK; G protein, monomeric; G protein

Chromosomal Location of Human Ortholog: 16q22

Cellular Component: intracellular; plasma membrane

Molecular Function: calmodulin binding; GTP binding; GTPase activity; protein binding

Biological Process: negative regulation of cell growth; protein transport; small GTPase mediated signal transduction
Show more

Research Articles on RRAD

1. Data show that Ras-related associated with diabetes protein (RRAD) directly binds to the p65 subunit of the NF-kappa B (NF-kappaB)complex and inhibits the nuclear translocation of p65, which in turn negatively regulates the NF-kappaB signaling

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Pathways associated with anti-RRAD antibody

 Products by Pathway  Pathway Diagram
 Insulin Signaling Pathway antibodies  Insulin Signaling Pathway Diagram

Diseases associated with anti-RRAD antibody

 Disease Name  Pubmed Publications
 Lung Neoplasms Antibodies  >7 publications with RRAD and Lung Neoplasms
 Lung Diseases Antibodies  >6 publications with RRAD and Lung Diseases
 Disease Models, Animal Antibodies  >5 publications with RRAD and Disease Models, Animal
 Carcinoma, Non-Small-Cell Lung Antibodies  >5 publications with RRAD and Carcinoma, Non-Small-Cell Lung
 Inflammation Antibodies  >4 publications with RRAD and Inflammation
 Necrosis Antibodies  >4 publications with RRAD and Necrosis
 Hypertrophy Antibodies  >4 publications with RRAD and Hypertrophy
 Adenocarcinoma Antibodies  >3 publications with RRAD and Adenocarcinoma
 Cell Transformation, Neoplastic Antibodies  >3 publications with RRAD and Cell Transformation, Neoplastic
 Liver Neoplasms Antibodies  >2 publications with RRAD and Liver Neoplasms

Organs/Tissues associated with anti-RRAD antibody

 Organ/Tissue Name  Pubmed Publications
 Muscle Antibodies  >44 publications with RRAD and Muscle
 Heart Antibodies  >19 publications with RRAD and Heart
 Lung Antibodies  >9 publications with RRAD and Lung
 Brain Antibodies  >6 publications with RRAD and Brain
 Embryonic Tissue Antibodies  >4 publications with RRAD and Embryonic Tissue
 Connective Tissue Antibodies  >3 publications with RRAD and Connective Tissue
 Kidney Antibodies  >3 publications with RRAD and Kidney
 Liver Antibodies  >3 publications with RRAD and Liver
 Prostate Antibodies  >2 publications with RRAD and Prostate
 Pancreas Antibodies  >2 publications with RRAD and Pancreas
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