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GEM Antibody – Goat Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS423162

MBS423162 is an antibody targeting GEM. Also known as Goat anti-GEM (aa34-46) Antibody. Reactive in Tested: Human; Expected from sequence similarity: Human. Host: Goat. More details

$450 / 0.1 mg +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
GEM, Polyclonal Antibody
Full Product Name
Goat anti-GEM (aa34-46) Antibody
Product Gene Name
anti-GEM antibody
Product Synonym Names
Goat Anti-GEM / KIR Antibody; GEM; GTP binding protein overexpressed in skeletal muscle; KIR; MGC26294; GTP-binding mitogen-induced T-cell protein; GTP-binding protein GEM; GTP-binding protein overexpressed in skeletal muscle; OTTHUMP00000226600; OTTHUMP00000226601; RAS-like protein K; GEM antibody; GTP binding protein overexpressed in skeletal muscle antibody; GTP-binding mitogen-induced T-cell protein antibody; GTP-binding protein GEM antibody; GTP-binding protein overexpressed in skeletal muscle antibody; kinase-inducible Ras-like protein antibody; KIR antibody; MGC26294 antibody; OTTHUMP00000226600 antibody; OTTHUMP00000226601 antibody; RAS-like protein KIR antibody; GEM (aa34-46)
Immunogen Sequence
QKEPHQYSHR NRH
Clonality
Polyclonal
Host
Goat
Species Reactivity
Tested: Human; Expected from sequence similarity: Human
Form/Format
Supplied at 0.5 mg/ml in Tris saline, 0. 02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Purity/Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Concentration
100ug specific antibody in 200ul (lot specific)
Preparation and Storage
Aliquot and store at -20 degree C. Minimize freezing and thawing.
Antibody/Peptide Pairs
GEM peptide (MBS427901) is used for blocking the activity of GEM antibody (MBS423162)
Molecular Weight
33,949 Da
OMIM
600164
Immunogen
Peptide with sequence C-QKEPHQYSHRNRH, from the internal region of the protein sequence according to NP_005252.1.
Epitope
Internal region
Note
Reported variants represent identical protein: NP_005252.1, NP_859053.1
ISO Certification
Manufactured in an ISO 9001:2008 Certified Laboratory.
Applications Tested/Suitable for anti-GEM antibody
Peptide ELISA (EIA), Western Blot (WB)
Application Notes for anti-GEM antibody
Peptide ELISA: Antibody detection limit dilution 1: 16000.
Western Blot: In transfected HEK293 transiently expressing Human recombinant GEM bands of approx.35+45kDa are observed. These bands are not observed in the transfected HEK293 with empty vector and the antibody does not cross-react to related protein such as Rem, Rem2L or Rad. Data provided by Prof. R Andres, Univ. Kentucky, USA. A similar banding pattern we observed in Human Spleen. The calculated molecular size is 33.9kDa according to NP_005252.1. Recommended concentration: 1-3ug/ml.

Western Blot (WB) of anti-GEM antibody

HEK293 lysate overexpressing full-length Human GEM (HA tagged), mock-transfected HEK293 (EV) and HEK293 transiently expressing GEM-related genes (Rem, Rem2L and Rad) probed with (1ug/ml). The same lysates probed with anti-HA tag antibody in the lower panel.
Goat Anti-GEM Polyclonal Antibody – Cat# MBS423162

Western Blot (WB) of anti-GEM antibody

(1ug/ml) staining of Human Spleen lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Goat Anti-GEM Polyclonal Antibody, Western Blot (WB) – Cat# MBS423162

Other Notes

Small volumes of anti-GEM antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for GEM. It may not necessarily be applicable to this product.

NCBI GI #
4885263
NCBI GeneID
2669
NCBI Accession #
NP_005252.1
UniProt Secondary Accession #
B2RA31
UniProt Related Accession #
P55040
GenBank Nucleotide #
NM_005261.3
NCBI Official Full Name
GTP-binding protein GEM
NCBI Official Synonym Full Names
GTP binding protein overexpressed in skeletal muscle
NCBI Official Symbol
GEM
NCBI Official Synonym Symbols
KIR
UniProt Protein Name
GTP-binding protein GEM
UniProt Synonym Protein Names
GTP-binding mitogen-induced T-cell protein; RAS-like protein KIR
Protein Family
GLABRA2 expression modulator
UniProt Gene Name
GEM
UniProt Synonym Gene Names
KIR
UniProt Entry Name
GEM_HUMAN
NCBI Protein Information
GTP-binding protein GEM

NCBI Summary for GEM

The protein encoded by this gene belongs to the RAD/GEM family of GTP-binding proteins. It is associated with the inner face of the plasma membrane and could play a role as a regulatory protein in receptor-mediated signal transduction. Alternative splicing occurs at this locus and two transcript variants encoding the same protein have been identified. [provided by RefSeq, Jul 2008]
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UniProt Comments for GEM

GEM: Could be a regulatory protein, possibly participating in receptor-mediated signal transduction at the plasma membrane. Has guanine nucleotide-binding activity but undetectable intrinsic GTPase activity. Belongs to the small GTPase superfamily. RGK family.

Protein type: G protein, monomeric, RGK; G protein; G protein, monomeric

Chromosomal Location of Human Ortholog: 8q13-q21

Cellular Component: internal side of plasma membrane; midbody; nucleus; spindle midzone

Molecular Function: calmodulin binding; GDP binding; GTP binding; GTPase activity; magnesium ion binding; protein binding

Biological Process: cell surface receptor linked signal transduction; chromosome organization and biogenesis; immune response; metaphase plate congression; mitosis; protein transport; signal transduction; small GTPase mediated signal transduction
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Research Articles on GEM

1. These findings demonstrate a new role of Gem/Gmip/RhoA signaling in cortical actin regulation during early mitotic stages.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-GEM antibody

 Reference Product  PubMed Publications
 PLK1 antibody  >2 publications with GEM and PLK1
 KIF9 antibody  >2 publications with GEM and KIF9
 CDC42 antibody  >2 publications with GEM and CDC42

Diseases associated with anti-GEM antibody

 Disease Name  Pubmed Publications
 Lung Neoplasms Antibodies  >179 publications with GEM and Lung Neoplasms
 Lung Diseases Antibodies  >171 publications with GEM and Lung Diseases
 Disease Models, Animal Antibodies  >139 publications with GEM and Disease Models, Animal
 Liver Neoplasms Antibodies  >132 publications with GEM and Liver Neoplasms
 Nervous System Diseases Antibodies  >131 publications with GEM and Nervous System Diseases
 Cardiovascular Diseases Antibodies  >122 publications with GEM and Cardiovascular Diseases
 Neoplasm Metastasis Antibodies  >118 publications with GEM and Neoplasm Metastasis
 Neoplasms, Experimental Antibodies  >113 publications with GEM and Neoplasms, Experimental
 Liver Diseases Antibodies  >99 publications with GEM and Liver Diseases
 Anemia Antibodies  >87 publications with GEM and Anemia

Organs/Tissues associated with anti-GEM antibody

 Organ/Tissue Name  Pubmed Publications
 Blood Antibodies  >404 publications with GEM and Blood
 Liver Antibodies  >247 publications with GEM and Liver
 Lung Antibodies  >222 publications with GEM and Lung
 Bone Antibodies  >105 publications with GEM and Bone
 Brain Antibodies  >105 publications with GEM and Brain
 Pancreas Antibodies  >93 publications with GEM and Pancreas
 Vascular Antibodies  >87 publications with GEM and Vascular
 Muscle Antibodies  >76 publications with GEM and Muscle
 Heart Antibodies  >65 publications with GEM and Heart
 Skin Antibodies  >64 publications with GEM and Skin
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