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RPA1 Antibody – Goat Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS423247

MBS423247 is an antibody targeting RPA1. Also known as Goat anti-RPA1/RPA70 (aa323-337) Antibody. Reactive in Tested: Human, Mouse; Expected from sequence similarity: Human. Host: Goat. More details

$450 / 0.1 mg +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Product Overview

Online Datasheet in PDF
Product Name
RPA1/RPA70, Polyclonal Antibody
Full Product Name
Goat anti-RPA1/RPA70 (aa323-337) Antibody
Product Gene Name
anti-RPA1 antibody
Product Synonym Gene Name
RPA70
Product Synonym Names
RPA1; replication protein A1, 70kDa; HSSB; MST075; REPA1; RF-A; RP-A; RPA70; MSTP075; RF-A protein 1; RP-A p70; replication factor A protein 1; replication protein A 70 kDa DNA-binding subunit; single-stranded DNA-binding protein; RPA1 antibody; replication protein A1; 70kDa antibody; HSSB antibody; MST075 antibody; REPA1 antibody; RF-A antibody; RP-A antibody; RPA70 antibody; MSTP075 antibody; RF-A protein 1 antibody; RP-A p70 antibody; replication factor A protein 1 antibody; replication protein A 70 kDa DNA-binding subunit antibody; single-stranded DNA-binding protein antibody; RPA1 / RPA70 (aa323-337)
Immunogen Sequence
CKSYEDATKI TVRSN
Clonality
Polyclonal
Host
Goat
Species Reactivity
Tested: Human, Mouse; Expected from sequence similarity: Human
Form/Format
Supplied at 0.5 mg/ml in Tris saline, 0. 02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Purity/Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Concentration
100ug specific antibody in 200ul (lot specific)
Preparation and Storage
Aliquot and store at -20 degree C. Minimize freezing and thawing.
Antibody/Peptide Pairs
RPA1 / RPA70 peptide (MBS428100) is used for blocking the activity of RPA1/RPA70 antibody (MBS423247)
Molecular Weight
68,138 Da
OMIM
179835
Immunogen
Peptide with sequence CKSYEDATKITVRSN, from the internal region of the protein sequence according to NP_002936.1.
Epitope
Internal region
ISO Certification
Manufactured in an ISO 9001:2008 Certified Laboratory.
Applications Tested/Suitable for anti-RPA1 antibody
Peptide ELISA (EIA), Western Blot (WB)
Application Notes for anti-RPA1 antibody
Peptide ELISA: Antibody detection limit dilution 1: 128000.
Western Blot: Approx 70+80kDa band observed in lysates of cell line HeLa (calculated MW of 68.1kDa according to NP_002936.1). The upper band likely represents the acetylated protein. A 75kDa band was observed in several Mouse cell lines and in lysates of Mouse Lymph Node. Recommended concentration: 0.3-1ug/ml.

Western Blot (WB) of anti-RPA1 antibody

(0.3ug/ml) staining of HeLa lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Goat Anti-RPA1 Polyclonal Antibody – Cat# MBS423247

Western Blot (WB) of anti-RPA1 antibody

(0.5ug/ml) staining of NIH3T3 (A), 3T3L1 (B), Mouse Lymph Node (C) and NSO (D) lysates (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Goat Anti-RPA1 Polyclonal Antibody, Western Blot (WB) – Cat# MBS423247

Other Notes

Small volumes of anti-RPA1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for RPA1. It may not necessarily be applicable to this product.

NCBI GI #
4506583
NCBI GeneID
6117
NCBI Accession #
NP_002936.1
UniProt Secondary Accession #
Q59ES9; A8K0Y9
UniProt Related Accession #
P27694
GenBank Nucleotide #
NM_002945.3
NCBI Official Full Name
replication protein A 70 kDa DNA-binding subunit
NCBI Official Synonym Full Names
replication protein A1
NCBI Official Symbol
RPA1
NCBI Official Synonym Symbols
HSSB; RF-A; RP-A; REPA1; RPA70; MST075
UniProt Protein Name
Replication protein A 70 kDa DNA-binding subunit
UniProt Synonym Protein Names
Replication factor A protein 1; RF-A protein 1
Protein Family
DNA-directed RNA polymerase
UniProt Gene Name
RPA1
UniProt Synonym Gene Names
REPA1; RPA70; RP-A p70; RF-A protein 1
UniProt Entry Name
RFA1_HUMAN
NCBI Protein Information
replication protein A 70 kDa DNA-binding subunit

UniProt Comments for RPA1

RPA1: Plays an essential role in several cellular processes in DNA metabolism including replication, recombination and DNA repair. Binds and subsequently stabilizes single-stranded DNA intermediates and thus prevents complementary DNA from reannealing. Heterotrimer composed of RPA1, RPA2 and RPA3 (canonical replication protein A complex). Component of the alternative replication protein A complex (aRPA) composed of RPA1, RPA3 and RPA4. The DNA-binding activity may reside exclusively on the RPA1 subunit. Interacts with RIPK1 and XPA. Interacts with RPA4. Interacts with the polymerase alpha subunit POLA1/p180; this interaction stabilizes the replicative complex and reduces the misincorporation rate of DNA polymerase alpha by acting as a fidelity clamp. Interact with RAD51 and SENP6 to regulate DNA repair. Interacts with HELB; this interaction promotes HELB recruitement to chromatin following DNA damage. Belongs to the replication factor A protein 1 family.

Protein type: DNA-binding

Chromosomal Location of Human Ortholog: 17p13.3

Cellular Component: DNA replication factor A complex; nuclear chromosome, telomeric region; nucleoplasm; nucleus; PML body

Molecular Function: damaged DNA binding; metal ion binding; protein binding; single-stranded DNA binding

Biological Process: base-excision repair; bypass DNA synthesis; DNA damage response, detection of DNA damage; DNA recombination; DNA repair; DNA replication; DNA-dependent DNA replication; double-strand break repair via homologous recombination; error-prone postreplication DNA repair; G1/S transition of mitotic cell cycle; mismatch repair; nucleotide-excision repair; nucleotide-excision repair, DNA gap filling; nucleotide-excision repair, DNA incision; nucleotide-excision repair, DNA incision, 3'-to lesion; nucleotide-excision repair, DNA incision, 5'-to lesion; nucleotide-excision repair, preincision complex assembly; nucleotide-excision repair, preincision complex stabilization; protein sumoylation; telomere maintenance; telomere maintenance via recombination; transcription-coupled nucleotide-excision repair
Show more

Research Articles on RPA1

1. BRG1-RAD52 complex mediates the replacement of RPA with RAD51 on single-stranded DNA (ssDNA) to initiate DNA strand invasion. Loss of BRG1 results in a failure of RAD51 loading onto ssDNA, abnormal homologous recombination repair

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-RPA1 antibody

 Reference Product  PubMed Publications
 PCNA antibody  >65 publications with RPA1 and PCNA
 XPA antibody  >62 publications with RPA1 and XPA
 RPA2 antibody  >61 publications with RPA1 and RPA2
 ATR antibody  >51 publications with RPA1 and ATR
 RAD51 antibody  >49 publications with RPA1 and RAD51
 RPA3 antibody  >33 publications with RPA1 and RPA3
 RAD52 antibody  >16 publications with RPA1 and RAD52
 BLM antibody  >13 publications with RPA1 and BLM
 DNA2 antibody  >5 publications with RPA1 and DNA2
 RPA4 antibody  >5 publications with RPA1 and RPA4

Pathways associated with anti-RPA1 antibody

 Products by Pathway  Pathway Diagram
 Activation Of ATR In Response To Replication Stress Pathway antibodies  Activation Of ATR In Response To Replication Stress Pathway Diagram
 Activation Of The Pre-replicative Complex Pathway antibodies  Activation Of The Pre-replicative Complex Pathway Diagram
 Base Excision Repair Pathway antibodies  Base Excision Repair Pathway Diagram
 Cell Cycle Pathway antibodies  Cell Cycle Pathway Diagram
 Cell Cycle Checkpoints Pathway antibodies  Cell Cycle Checkpoints Pathway Diagram
 Cell Cycle, Mitotic Pathway antibodies  Cell Cycle, Mitotic Pathway Diagram
 Cellular Response To Heat Stress Pathway antibodies  Cellular Response To Heat Stress Pathway Diagram
 Cellular Responses To Stress Pathway antibodies  Cellular Responses To Stress Pathway Diagram
 Chromosome Maintenance Pathway antibodies  Chromosome Maintenance Pathway Diagram
 DNA Damage Bypass Pathway antibodies  DNA Damage Bypass Pathway Diagram

Diseases associated with anti-RPA1 antibody

 Disease Name  Pubmed Publications
 Carcinoma Antibodies  >24 publications with RPA1 and Carcinoma
 Precancerous Conditions Antibodies  >23 publications with RPA1 and Precancerous Conditions
 Breast Neoplasms Antibodies  >13 publications with RPA1 and Breast Neoplasms
 Vascular Diseases Antibodies  >10 publications with RPA1 and Vascular Diseases
 Cardiovascular Diseases Antibodies  >10 publications with RPA1 and Cardiovascular Diseases
 Abnormalities, Multiple Antibodies  >8 publications with RPA1 and Abnormalities, Multiple
 Anemia Antibodies  >7 publications with RPA1 and Anemia
 Adenocarcinoma Antibodies  >6 publications with RPA1 and Adenocarcinoma
 Lung Neoplasms Antibodies  >5 publications with RPA1 and Lung Neoplasms
 Ovarian Neoplasms Antibodies  >4 publications with RPA1 and Ovarian Neoplasms

Organs/Tissues associated with anti-RPA1 antibody

 Organ/Tissue Name  Pubmed Publications
 Blood Antibodies  >22 publications with RPA1 and Blood
 Brain Antibodies  >11 publications with RPA1 and Brain
 Skin Antibodies  >9 publications with RPA1 and Skin
 Lung Antibodies  >8 publications with RPA1 and Lung
 Ovary Antibodies  >6 publications with RPA1 and Ovary
 Liver Antibodies  >6 publications with RPA1 and Liver
 Lymph Node Antibodies  >5 publications with RPA1 and Lymph Node
 Placenta Antibodies  >4 publications with RPA1 and Placenta
 Lymph Antibodies  >4 publications with RPA1 and Lymph
 Bone Antibodies  >4 publications with RPA1 and Bone
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