Goat Anti-ROBO3 Polyclonal Antibody – Cat# MBS423254

ROBO3 Antibody – Goat Polyclonal

Name: ROBO3 Antibody; Polyclonal; Goat; Human-Reactive; MyBioSource
Brand: MyBioSource
SKU: MBS423254
Price: 450 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS423254

MBS423254 is an antibody targeting Robo3. Also known as Goat anti-Robo3.2 (mouse aa1303-1316) Antibody. Reactive in Expected from sequence similarity: Human, Mouse, Rat, Dog, Pig, Cow. Host: Goat. More details

$450 / 0.1 mg +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Online Datasheet in PDF

Product Name

Robo3.2, Polyclonal Antibody

Full Product Name

Goat anti-Robo3.2 (mouse aa1303-1316) Antibody

Product Gene Name

anti-Robo3 antibody

Product Synonym Names

Robo3; roundabout homolog 3 (Drosophila); Rbig1; Rig-1; Rig1; Robo3a; Robo3b; retinoblastoma inhibiting gene 1; retinoblastoma-inhibiting gene 1 protein; roundabout homolog 3; Rbig1 antibody; retinoblastoma inhibiting gene 1 antibody; retinoblastoma-inhibiting gene 1 protein antibody; Rig1 antibody; Rig-1 antibody; Robo3 antibody; Robo3a antibody; Robo3b antibody; roundabout homolog 3 antibody; roundabout homolog 3 (Drosophila) antibody; Robo3.2 (mouse aa1303-1316)

Immunogen Sequence

KESQGRGRGL EACR

Clonality

Polyclonal

Host

Goat

Species Reactivity

Expected from sequence similarity: Human, Mouse, Rat, Dog, Pig, Cow

Form/Format

Supplied at 0.5 mg/ml in Tris saline, 0. 02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

Purity/Purification

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Concentration

100ug specific antibody in 200ul (lot specific)

Preparation and Storage

Aliquot and store at -20 degree C. Minimize freezing and thawing.

Antibody/Peptide Pairs

Robo3.2 peptide (MBS428475) is used for blocking the activity of Robo3.2 antibody (MBS423254)

Molecular Weight

143,440 Da

Immunogen

Peptide with sequence KESQGRGRGLEACR, from the C-terminus of the protein sequence according to AAD11628.1.

Epitope

C-Terminus

ISO Certification

Manufactured in an ISO 9001:2008 Certified Laboratory.

Applications Tested/Suitable for anti-Robo3 antibody

Peptide ELISA (EIA)

Application Notes for anti-Robo3 antibody

Peptide ELISA: Antibody detection limit dilution 1: 128000.
Western Blot: Preliminary experiments gave bands at approx 85kDa in Mouse fetal Brain lysates after 1ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the bands we observe given the calculated size of 150kDa according to Mouse AAD11628.1. Both detected bands were successfully blocked by incubation with the immunizing peptide (and BLAST results with the immunizing peptide sequence did not identify any other proteins to explain the additional bands).

Other Notes

Small volumes of anti-Robo3 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for Robo3. It may not necessarily be applicable to this product.

NCBI GI #

4206386

NCBI GeneID

19649

NCBI Accession #

AAD11628.1

UniProt Related Accession #

Q9Z2I4

NCBI Official Full Name

rig-1 protein

NCBI Official Synonym Full Names

roundabout guidance receptor 3

NCBI Official Symbol

Robo3

NCBI Official Synonym Symbols

Rig1; Rbig1; Rig-1; Robo3a; Robo3b

UniProt Protein Name

Roundabout homolog 3

UniProt Synonym Protein Names

Retinoblastoma-inhibiting gene 1 protein; Rig-1

Protein Family

Roundabout

UniProt Gene Name

Robo3

UniProt Synonym Gene Names

Rbig1; Rig-1

UniProt Entry Name

ROBO3_MOUSE

NCBI Protein Information

roundabout homolog 3

UniProt Comments for Robo3

ROBO3: Thought to be involved during neural development in axonal navigation at the ventral midline of the neural tube. In spinal chord development plays a role in guiding commissural axons probably by preventing premature sensitivity to Slit proteins thus inhibiting Slit signaling through ROBO1. Required for hindbrain axon midline crossing. Defects in ROBO3 are a cause of familial horizontal gaze palsy with progressive scoliosis (HGPPS). Patients show a medulla where motor and sensory projections appear uncrossed. Belongs to the immunoglobulin superfamily. ROBO family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral

Cellular Component: axon; integral to membrane; membrane

Molecular Function: protein binding

Biological Process: axon guidance; axon midline choice point recognition; cell differentiation; chemotaxis; multicellular organismal development; nervous system development; neuron migration

Research Articles on Robo3

1. This study show that this commissural neuron population includes spinal inhibitory neurons and sensory nociceptors and show that this population depends on Robo/Slit signaling yet crosses the dorsal midline in a Robo3-independent manner.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-Robo3 antibody

Reference Product	PubMed Publications
Robo1 antibody	>29 publications with Robo3 and Robo1
Slit1 antibody	>21 publications with Robo3 and Slit1
Slit2 antibody	>16 publications with Robo3 and Slit2
Slit3 antibody	>11 publications with Robo3 and Slit3
Ntn1 antibody	>3 publications with Robo3 and Ntn1

Pathways associated with anti-Robo3 antibody

Products by Pathway	Pathway Diagram
Axon Guidance Pathway antibodies	Axon Guidance Pathway Diagram
Axon Guidance Pathway antibodies	Axon Guidance Pathway Diagram
Axon Guidance Pathway antibodies	Axon Guidance Pathway Diagram
Developmental Biology Pathway antibodies	Developmental Biology Pathway Diagram
Regulation Of Commissural Axon Pathfinding By Slit And Robo Pathway antibodies	Regulation Of Commissural Axon Pathfinding By Slit And Robo Pathway Diagram
Signaling By Robo Receptor Pathway antibodies	Signaling By Robo Receptor Pathway Diagram

Organs/Tissues associated with anti-Robo3 antibody

Organ/Tissue Name	Pubmed Publications
Brain Antibodies	>59 publications with Robo3 and Brain
Embryonic Tissue Antibodies	>30 publications with Robo3 and Embryonic Tissue
Spinal Cord Antibodies	>25 publications with Robo3 and Spinal Cord
Eye Antibodies	>23 publications with Robo3 and Eye
Liver Antibodies	>4 publications with Robo3 and Liver
Ovary Antibodies	>3 publications with Robo3 and Ovary