NANOG Antibody – Goat Polyclonal

The protein encoded by this gene is a DNA binding homeobox transcription factor involved in embryonic stem (ES) cell proliferation, renewal, and pluripotency. The encoded protein can block ES cell differentiation and can also autorepress its own expression in differentiating cells. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2015]

NANOG: Transcription regulator involved in inner cell mass and embryonic stem (ES) cells proliferation and self-renewal. Imposes pluripotency on ES cells and prevents their differentiation towards extraembryonic endoderm and trophectoderm lineages. Blocks bone morphogenetic protein-induced mesoderm differentiation of ES cells by physically interacting with SMAD1 and interfering with the recruitment of coactivators to the active SMAD transcriptional complexes. Acts as a transcriptional activator or repressor. Binds optimally to the DNA consensus sequence 5'-TAAT[GT][GT]-3' or 5'-[CG][GA][CG]C[GC]ATTAN[GC]-3'. When overexpressed, promotes cells to enter into S phase and proliferation. Interacts with SMAD1 and SALL4. Expressed in testicular carcinoma and derived germ cell tumors. Expressed in fetal gonads, ovary and testis. Also expressed in ovary teratocarcinoma cell line and testicular embryonic carcinoma. Not expressed in many somatic organs and oocytes. Belongs to the Nanog homeobox family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Transcription factor; Cell development/differentiation; DNA-binding

Cellular Component: nucleolus; nucleoplasm; nucleus

Molecular Function: chromatin binding; DNA binding; protein binding; sequence-specific DNA binding; transcription corepressor activity; transcription factor activity

Biological Process: cell proliferation; embryonic development; endodermal cell fate specification; mesodermal cell fate commitment; multicellular organismal development; negative regulation of BMP signaling pathway; negative regulation of cell differentiation; negative regulation of cell fate commitment; negative regulation of endodermal cell fate specification; negative regulation of transcription from RNA polymerase II promoter; positive regulation of cell proliferation; positive regulation of mitotic cell cycle; positive regulation of transcription from RNA polymerase II promoter; positive regulation of transcription, DNA-dependent; regulation of cell differentiation; regulation of gene expression; regulation of transcription, DNA-dependent; response to organic substance; response to retinoic acid; somatic stem cell maintenance; stem cell differentiation; stem cell division; stem cell maintenance; transcription, DNA-dependent