• Call +1.858.633.0165 or Fax +1.858.633.0166 or Contact Us

Betacstf-64 Variant 1/ Betacstf-64 Variant 3 (CSTF2) Antibody – Goat Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS423289

MBS423289 is an antibody targeting Cstf2. Also known as Goat anti-betaCstF-64 variant 1/ betaCstF-64 variant 3 (mouse) Antibody. Reactive in Tested: Mouse; Expected from sequence similarity: Mouse. Host: Goat. More details

$450 / 0.1 mg +1 FREE 8GB USB
Product Documentation
PDF
Sample Manual Insert View PDF
PDF
Testing Data View PDF
PDF
Certificate of Analysis (COA) View PDF
PDF
QC Report View PDF
PDF
Material Safety Data Sheet View PDF
PDF
Technical Datasheet View PDF
PDF
Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
betaCstF-64 variant 1/ betaCstF-64 variant 3 (Cstf2), Polyclonal Antibody
Full Product Name
Goat anti-betaCstF-64 variant 1/ betaCstF-64 variant 3 (mouse) Antibody
Product Gene Name
anti-Cstf2 antibody
Product Synonym Names
Cstf2; cleavage stimulation factor, 3' pre-RNA subunit 2; 64kDa; C630034J23Rik; Cstf64; CF-1 64 kDa subunit; CSTF 64 kDa subunit; alphaCstF-64 variant 4; betaCstF-64 variant 1; betaCstF-64 variant 3; cleavage stimulation factor 64 kDa subunit; cleavage st; 64kDa antibody; alphaCstF-64 variant 4 antibody; betaCstF-64 variant 1 antibody; betaCstF-64 variant 3 antibody; C630034J23Rik antibody; CF-1 64 kDa subunit antibody; cleavage stimulation factor 64 kDa subunit antibody; cleavage stimulation factor subunit 2 antibody; cleavage stimulation factor; 3' pre-RNA subunit 2 antibody; cleavage stimulation factor; 3' pre-RNA subunit 2; 64 kDa antibody; CSTF 64 kDa subunit antibody; Cstf2 antibody; Cstf64 antibody; cstF-64 antibody; betaCstF-64 variant 1; betaCstF-64 variant 3; betaCstF-64 variant 1/ betaCstF-64 variant 3 (mouse)
Immunogen Sequence
PASIERVQGQ RT
Clonality
Polyclonal
Host
Goat
Species Reactivity
Tested: Mouse; Expected from sequence similarity: Mouse
Form/Format
Supplied at 0.5 mg/ml in Tris saline, 0. 02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Purity/Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Concentration
100ug specific antibody in 200ul (lot specific)
Preparation and Storage
Aliquot and store at -20 degree C. Minimize freezing and thawing.
Antibody/Peptide Pairs
betaCstF-64 variant 1/ betaCstF-64 variant 3 peptide (MBS428016) is used for blocking the activity of betaCstF-64 variant 1/ betaCstF-64 variant 3 antibody (MBS423289)
Molecular Weight
54,069 Da
Immunogen
Peptide with sequence C-PASIERVQGQRT, from the internal region of the protein sequence according to ACF05702.1; ACF05700.1.
Epitope
Internal region
Note
This antibody recognizes mouse variants 1 and 3 of the reported beta isoform (ACF05702.1; ACF05700.1).
ISO Certification
Manufactured in an ISO 9001:2008 Certified Laboratory.
Applications Tested/Suitable for anti-Cstf2 antibody
Peptide ELISA (EIA), Immunoprecipitation (IP)
Application Notes for anti-Cstf2 antibody
Peptide ELISA: Antibody detection limit dilution 1: 128000.
Immunoprecipitation: An approx 75kDa band is observed when endogenously expressed betaCstf-64 is pulled down from Mouse Brain lysates using while Cstf-64 which is endogenously expresed in Mouse Liver, is not pulled down. Western blot was probed with a mouse monoclonal antibody recognizing both betaCstf-64 anf Cstf-64 Recommended amount 2ug lysate protein.

Western Blot (WB) of anti-Cstf2 antibody

HeLa lysate either in RIPA buffer (lanes 1, 2, 4, 6) or TX100 buffer (lanes 3, 5, 7) overexpressing Mouse betaCstf-64 (DYKDDDDK tagged) immunoprecipitated using (2ug) with Protein G-coated magnetic beads in lanes 6 and 7 and probed with anti-DYKDDDDK (1/1000). Mock-transfected HeLa in Lane 1. A 1/40th part of the lysate was loaded in lanes 2 and 3 before IP and in lanes 4 an 5 after IP. Primary incubations were for 1 hour. Detected by chemiluminescence. Data obtained from Dr P Grozdanov, Texas Tech University Health Sciences Center, USA.
Goat Anti-CSTF2 Polyclonal Antibody – Cat# MBS423289

Western Blot (WB) of anti-Cstf2 antibody

(2ug) used to pull down betaCstf-64 from Mouse Brain (lane 5) lysate (500ug protein) while it cannot pull down Cstf-64 from Mouse Liver (lane 6) lysate (500ug) protein) using Protein G-coated magnetic beads. A 1/40th part of the lysates were loaded in lanes 1 (Brain) and 2 (Liver) before IP and in lanes 3 (Brain) and 4 (Liver) after IP. Western blot probed with mouse anti-betaCstf-64/Cstf-64 antibody and detected by chemiluminescence. Data obtained from Dr P Grozdanov, Texas Tech University Health Sciences Center, USA.
Goat Anti-CSTF2 Polyclonal Antibody, Western Blot (WB) – Cat# MBS423289

Other Notes

Small volumes of anti-Cstf2 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for Cstf2. It may not necessarily be applicable to this product.

NCBI GI #
192764316
NCBI GeneID
108062
NCBI Accession #
ACF05702.1
NCBI Related Accession #
ACF05700.1
UniProt Secondary Accession #
Q8K1Y6; Q9ERC2; A2AEJ9; A2AEK0
UniProt Related Accession #
Q8BIQ5
NCBI Official Full Name
betaCstF-64 variant 1
NCBI Official Synonym Full Names
cleavage stimulation factor, 3' pre-RNA subunit 2
NCBI Official Symbol
Cstf2
NCBI Official Synonym Symbols
Cstf64; mir-3112; C630034J23Rik
UniProt Protein Name
Cleavage stimulation factor subunit 2
UniProt Synonym Protein Names
CF-1 64 kDa subunit; Cleavage stimulation factor 64 kDa subunit; CSTF 64 kDa subunit; CstF-64
Protein Family
Cleavage stimulation factor
UniProt Gene Name
Cstf2
UniProt Synonym Gene Names
CSTF 64 kDa subunit; CstF-64
UniProt Entry Name
CSTF2_MOUSE
NCBI Protein Information
cleavage stimulation factor subunit 2

UniProt Comments for Cstf2

CSTF2: One of the multiple factors required for polyadenylation and 3'-end cleavage of mammalian pre-mRNAs. This subunit is directly involved in the binding to pre-mRNAs. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: RNA-binding; RNA splicing

Cellular Component: mRNA cleavage and polyadenylation specificity factor complex; nucleoplasm; nucleus

Molecular Function: mRNA binding; nucleic acid binding; nucleotide binding; RNA binding

Biological Process: mRNA 3'-end processing; mRNA polyadenylation; mRNA processing
Show more

Research Articles on Cstf2

1. The results suggest that CstF-64 plays a key role in modulating the cell cycle in embryonic stem cells while simultaneously controlling histone mRNA 3' end processing.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Pathways associated with anti-Cstf2 antibody

 Products by Pathway  Pathway Diagram
 Cleavage Of Growing Transcript In The Termination Region Pathway antibodies  Cleavage Of Growing Transcript In The Termination Region Pathway Diagram
 Gene Expression Pathway antibodies  Gene Expression Pathway Diagram
 Processing Of Capped Intron-Containing Pre-mRNA Pathway antibodies  Processing Of Capped Intron-Containing Pre-mRNA Pathway Diagram
 Processing Of Capped Intronless Pre-mRNA Pathway antibodies  Processing Of Capped Intronless Pre-mRNA Pathway Diagram
 Processing Of Intronless Pre-mRNAs Pathway antibodies  Processing Of Intronless Pre-mRNAs Pathway Diagram
 RNA Polymerase II Transcription Pathway antibodies  RNA Polymerase II Transcription Pathway Diagram
 RNA Polymerase II Transcription Termination Pathway antibodies  RNA Polymerase II Transcription Termination Pathway Diagram
 MRNA 3'-end Processing Pathway antibodies  MRNA 3'-end Processing Pathway Diagram
 MRNA Splicing Pathway antibodies  MRNA Splicing Pathway Diagram
 MRNA Splicing - Major Pathway antibodies  MRNA Splicing - Major Pathway Diagram

Diseases associated with anti-Cstf2 antibody

 Disease Name  Pubmed Publications
 Abnormalities, Multiple Antibodies  >1 publications with Cstf2 and Abnormalities, Multiple
 Congenital Abnormalities Antibodies  >1 publications with Cstf2 and Congenital Abnormalities
 Hyperplasia Antibodies  >1 publications with Cstf2 and Hyperplasia
 Necrosis Antibodies  >1 publications with Cstf2 and Necrosis

Organs/Tissues associated with anti-Cstf2 antibody

 Organ/Tissue Name  Pubmed Publications
 Brain Antibodies  >3 publications with Cstf2 and Brain
 Testis Antibodies  >2 publications with Cstf2 and Testis
 Liver Antibodies  >1 publications with Cstf2 and Liver
 Extraembryonic Tissue Antibodies  >1 publications with Cstf2 and Extraembryonic Tissue
 Lung Antibodies  >1 publications with Cstf2 and Lung
 Embryonic Tissue Antibodies  >1 publications with Cstf2 and Embryonic Tissue
Request a Quote

Please fill out the form below and our representative will get back to you shortly.

MBS000000
Contact Us

Please fill out the form below and our representative will get back to you shortly.

MBS000000