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POLRMT Antibody – Goat Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS423318

MBS423318 is an antibody targeting POLRMT. Also known as Goat anti-POLRMT Antibody. Reactive in Expected from sequence similarity: Human. Host: Goat. More details

$305 / 0.1 mg +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
POLRMT, Polyclonal Antibody
Full Product Name
Goat anti-POLRMT Antibody
Product Gene Name
Product Synonym Names
POLRMT; polymerase (RNA) mitochondrial (DNA directed); APOLMT; MTRNAP; MTRPOL; h-mtRPOL; DNA-directed RNA polymerase, mitochondrial; POLRMT antibody; polymerase (RNA) mitochondrial (DNA directed) antibody; APOLMT antibody; MTRNAP antibody; MTRPOL antibody; h-mtRPOL antibody; DNA-directed RNA polymerase; mitochondrial antibody
Immunogen Sequence
KETLQAVPKP GAFD
Clonality
Polyclonal
Host
Goat
Species Reactivity
Expected from sequence similarity: Human
Form/Format
Supplied at 0.5 mg/ml in Tris saline, 0. 02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Purity/Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Concentration
100ug specific antibody in 200ul (lot specific)
Preparation and Storage
Aliquot and store at -20 degree C. Minimize freezing and thawing.
Antibody/Peptide Pairs
POLRMT peptide (MBS428361) is used for blocking the activity of POLRMT antibody (MBS423318)
Molecular Weight
138,620 Da
OMIM
601778
Immunogen
Peptide with sequence C-KETLQAVPKPGAFD, from the C Terminus of the protein sequence according to NP_005026.3.
Epitope
C Terminus
ISO Certification
Manufactured in an ISO 9001:2008 Certified Laboratory.
Applications Tested/Suitable for anti-POLRMT antibody
Peptide ELISA (EIA)
Application Notes for anti-POLRMT antibody
Peptide ELISA: Antibody detection limit dilution 1: 128000.
Western Blot: Preliminary experiments gave bands at approx 90kDa and 35kDa in Human HepG2 lysates after 1ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the bands we observe given the calculated size of 139kDa according to NP_005026.3. Both detected bands were successfully blocked by incubation with the immunizing peptide (and BLAST results with the immunizing peptide sequence did not identify any other proteins to explain the additional bands).

Other Notes

Small volumes of anti-POLRMT antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for POLRMT. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Secondary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
DNA-directed RNA polymerase, mitochondrial
NCBI Official Synonym Full Names
polymerase (RNA) mitochondrial
NCBI Official Symbol
POLRMT
NCBI Official Synonym Symbols
APOLMT; MTRNAP; MTRPOL; h-mtRPOL
UniProt Protein Name
DNA-directed RNA polymerase, mitochondrial
UniProt Gene Name
POLRMT
UniProt Synonym Gene Names
MtRPOL
UniProt Entry Name
RPOM_HUMAN
NCBI Protein Information
DNA-directed RNA polymerase, mitochondrial

NCBI Summary for POLRMT

This gene encodes a mitochondrial DNA-directed RNA polymerase. The gene product is responsible for mitochondrial gene expression as well as for providing RNA primers for initiation of replication of the mitochondrial genome. Although this polypeptide has the same function as the three nuclear DNA-directed RNA polymerases, it is more closely related to RNA polymerases of phage and mitochondrial polymerases of lower eukaryotes. [provided by RefSeq, Jul 2008]
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UniProt Comments for POLRMT

POLRMT: DNA-dependent RNA polymerase catalyzes the transcription of mitochondrial DNA into RNA using the four ribonucleoside triphosphates as substrates. Belongs to the phage and mitochondrial RNA polymerase family.

Protein type: Transferase; Transcription initiation complex; EC 2.7.7.6; Mitochondrial

Chromosomal Location of Human Ortholog: 19p13.3

Cellular Component: mitochondrial matrix; mitochondrion

Molecular Function: DNA binding; DNA-directed RNA polymerase activity; protein binding

Biological Process: mitochondrion organization and biogenesis; transcription from mitochondrial promoter; transcription initiation from mitochondrial promoter
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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