Goat Anti-ITK Polyclonal Antibody – Cat# MBS423390

(0.3ug/ml) staining of Rat Thymus lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

ITK Antibody – Goat Polyclonal

Name: ITK Antibody; Polyclonal; Goat; Rat/Human-Reactive; MyBioSource
Brand: MyBioSource
SKU: MBS423390
Price: 450 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS423390

MBS423390 is an antibody targeting ITK. Also known as Goat anti-ITK Antibody. Reactive in Tested: Rat; Expected from sequence similarity: Human, Rat, Cow. Host: Goat. More details

$450 / 0.1 mg +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Online Datasheet in PDF

Product Name

ITK, Polyclonal Antibody

Full Product Name

Goat anti-ITK Antibody

Product Gene Name

anti-ITK antibody

Product Synonym Names

Goat Anti-ITK Antibody; ITK; IL2-inducible T-cell kinase; EMT; LYK; MGC126257; MGC126258; PSCTK2; homolog of mouse T-cell itk/tsk; tyrosine-protein kinase ITK/TSK; tyrosine-protein kinase LYK; ITK antibody; IL2-inducible T-cell kinase antibody; EMT antibody; LYK antibody; MGC126257 antibody; MGC126258 antibody; PSCTK2 antibody; homolog of mouse T-cell itk/tsk antibody; tyrosine-protein kinase ITK/TSK antibody; tyrosine-protein kinase LYK antibody

Immunogen Sequence

KERPEDRPAF SR

Clonality

Polyclonal

Host

Goat

Species Reactivity

Tested: Rat; Expected from sequence similarity: Human, Rat, Cow

Form/Format

Supplied at 0.5 mg/ml in Tris saline, 0. 02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

Purity/Purification

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Concentration

100ug specific antibody in 200ul (lot specific)

Preparation and Storage

Aliquot and store at -20 degree C. Minimize freezing and thawing.

Antibody/Peptide Pairs

ITK peptide (MBS428252) is used for blocking the activity of ITK antibody (MBS423390)

Molecular Weight

71,831 Da

OMIM

186973

Immunogen

Peptide with sequence C-KERPEDRPAFSR, from the internal (near C terminus) of the protein sequence according to NP_005537.3.

Epitope

Internal (near C Terminus)

ISO Certification

Manufactured in an ISO 9001:2008 Certified Laboratory.

Applications Tested/Suitable for anti-ITK antibody

Peptide ELISA (EIA), Western Blot (WB)

Application Notes for anti-ITK antibody

Peptide ELISA: Antibody detection limit dilution 1: 64000.
Western Blot: Approx 70kDa band observed in Rat Thymus lysates (calculated MW of 71.8kDa according to NP_005537.3). Recommended concentration: 0.3-1ug/ml.

Other Notes

Small volumes of anti-ITK antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for ITK. It may not necessarily be applicable to this product.

NCBI GI #

15718680

NCBI GeneID

3702

NCBI Accession #

NP_005537.3

UniProt Secondary Accession #

Q32ML7; B2R752

UniProt Related Accession #

Q08881

GenBank Nucleotide #

NM_005546.3

NCBI Official Full Name

tyrosine-protein kinase ITK/TSK

NCBI Official Synonym Full Names

IL2 inducible T-cell kinase

NCBI Official Symbol

ITK

NCBI Official Synonym Symbols

EMT; LYK; LPFS1; PSCTK2

UniProt Protein Name

Tyrosine-protein kinase ITK/TSK

UniProt Synonym Protein Names

Interleukin-2-inducible T-cell kinase; IL-2-inducible T-cell kinase; Kinase EMT; T-cell-specific kinase; Tyrosine-protein kinase Lyk

Protein Family

Tyrosine-protein kinase

UniProt Gene Name

ITK

UniProt Synonym Gene Names

EMT; LYK; IL-2-inducible T-cell kinase

UniProt Entry Name

ITK_HUMAN

NCBI Protein Information

tyrosine-protein kinase ITK/TSK

NCBI Summary for ITK

This gene encodes an intracellular tyrosine kinase expressed in T-cells. The protein contains both SH2 and SH3 domains which are often found in intracellular kinases. It is thought to play a role in T-cell proliferation and differentiation. [provided by RefSeq, Jul 2008]

UniProt Comments for ITK

ITK: an intracellular tyrosine kinase of the Tec family expressed in T-cells. The protein contains both SH2 and SH3 domains and plays a role in T-cell proliferation and differentiation. Itk-deficient murine T cells display defects in TCR/CD3-induced actin polymerization.

Protein type: Protein kinase, TK; Kinase, protein; Protein kinase, tyrosine (non-receptor); EC 2.7.10.2; TK group; Tec family

Chromosomal Location of Human Ortholog: 5q31-q32

Cellular Component: cytosol; extrinsic to internal side of plasma membrane; intercellular junction

Molecular Function: ATP binding; metal ion binding; non-membrane spanning protein tyrosine kinase activity; protein binding; receptor binding

Biological Process: adaptive immune response; cellular defense response; cytokine production; innate immune response; interferon-gamma production; interleukin-4 production; NK T cell differentiation; phospholipase C activation; regulation of cell proliferation; signal transduction; T cell activation; T cell receptor signaling pathway; transmembrane receptor protein tyrosine kinase signaling pathway

Disease: Lymphoproliferative Syndrome 1

Research Articles on ITK

1. We conclude that ITK, formerly considered an immune cell-specific protein, is aberrantly expressed in melanoma and promotes tumor development and progression

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-ITK antibody

Reference Product	PubMed Publications
SRC antibody	>113 publications with ITK and SRC
CD28 antibody	>48 publications with ITK and CD28
LAT antibody	>28 publications with ITK and LAT
FYN antibody	>23 publications with ITK and FYN
VAV1 antibody	>20 publications with ITK and VAV1
GRB2 antibody	>13 publications with ITK and GRB2
CBL antibody	>10 publications with ITK and CBL
PLCG1 antibody	>3 publications with ITK and PLCG1
KHDRBS1 antibody	>1 publications with ITK and KHDRBS1
LCP2 antibody	>1 publications with ITK and LCP2

Pathways associated with anti-ITK antibody

Products by Pathway	Pathway Diagram
Adaptive Immune System Pathway antibodies	Adaptive Immune System Pathway Diagram
B Cell Receptor Signaling Pathway antibodies	B Cell Receptor Signaling Pathway Diagram
Chemokine Signaling Pathway antibodies	Chemokine Signaling Pathway Diagram
Chemokine Signaling Pathway antibodies	Chemokine Signaling Pathway Diagram
Class I PI3K Signaling Events Pathway antibodies	Class I PI3K Signaling Events Pathway Diagram
FCERI Mediated Ca+2 Mobilization Pathway antibodies	FCERI Mediated Ca+2 Mobilization Pathway Diagram
Fc Epsilon Receptor (FCERI) Signaling Pathway antibodies	Fc Epsilon Receptor (FCERI) Signaling Pathway Diagram
Fc-epsilon Receptor I Signaling In Mast Cells Pathway antibodies	Fc-epsilon Receptor I Signaling In Mast Cells Pathway Diagram
Generation Of Second Messenger Molecules Pathway antibodies	Generation Of Second Messenger Molecules Pathway Diagram
Immune System Pathway antibodies	Immune System Pathway Diagram

Diseases associated with anti-ITK antibody

Disease Name	Pubmed Publications
Inflammation Antibodies	>44 publications with ITK and Inflammation
Lymphoma Antibodies	>34 publications with ITK and Lymphoma
Lung Diseases Antibodies	>28 publications with ITK and Lung Diseases
Neoplasms, Experimental Antibodies	>6 publications with ITK and Neoplasms, Experimental
Congenital Abnormalities Antibodies	>5 publications with ITK and Congenital Abnormalities
Necrosis Antibodies	>5 publications with ITK and Necrosis
Hyperplasia Antibodies	>5 publications with ITK and Hyperplasia
Lung Neoplasms Antibodies	>4 publications with ITK and Lung Neoplasms
Vascular Diseases Antibodies	>4 publications with ITK and Vascular Diseases
Liver Neoplasms Antibodies	>3 publications with ITK and Liver Neoplasms

Organs/Tissues associated with anti-ITK antibody

Organ/Tissue Name	Pubmed Publications
Blood Antibodies	>270 publications with ITK and Blood
Brain Antibodies	>43 publications with ITK and Brain
Thymus Antibodies	>39 publications with ITK and Thymus
Lung Antibodies	>30 publications with ITK and Lung
Spleen Antibodies	>23 publications with ITK and Spleen
Lymph Node Antibodies	>12 publications with ITK and Lymph Node
Intestine Antibodies	>11 publications with ITK and Intestine
Heart Antibodies	>11 publications with ITK and Heart
Prostate Antibodies	>7 publications with ITK and Prostate
Eye Antibodies	>5 publications with ITK and Eye