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SP140 Antibody – Goat Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS423403

MBS423403 is an antibody targeting SP140. Also known as Goat anti-SP140 (aa29-41) Antibody. Reactive in Tested: Human; Expected from sequence similarity: Human. Host: Goat. More details

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Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
SP140, Polyclonal Antibody
Full Product Name
Goat anti-SP140 (aa29-41) Antibody
Product Gene Name
Product Synonym Names
SP140; SP140 nuclear body protein; LYSP100; LYSP100-A; LYSP100-B; lymphoid-restricted homolog of Sp100; lymphoid-specific SP100 homolog; nuclear autoantigen Sp-140; nuclear body protein SP140; speckled 140 kDa; SP140 antibody; SP140 nuclear body protein antibody; LYSP100 antibody; LYSP100-A antibody; LYSP100-B antibody; lymphoid-restricted homolog of Sp100 antibody; lymphoid-specific SP100 homolog antibody; nuclear autoantigen Sp-140 antibody; nuclear body protein SP140 antibody; speckled 140 kDa antibody; SP140 (aa29-41)
Immunogen Sequence
EGQNLQEQVC PEP
Clonality
Polyclonal
Host
Goat
Species Reactivity
Tested: Human; Expected from sequence similarity: Human
Form/Format
Supplied at 0.5 mg/ml in Tris saline, 0. 02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Purity/Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Concentration
100ug specific antibody in 200ul (lot specific)
Preparation and Storage
Aliquot and store at -20 degree C. Minimize freezing and thawing.
Antibody/Peptide Pairs
SP140 peptide (MBS428646) is used for blocking the activity of SP140 antibody (MBS423403)
Molecular Weight
91,673 Da
OMIM
608602
Immunogen
Peptide with sequence EGQNLQEQVCPEP, from the N-Terminus of the protein sequence according to NP_009168.4; NP_001005176.1; NP_001265380.1; NP_001265381.1; NP_001265382.1.
Epitope
N-Terminus
Note
This antibody is expected to recognize all reported isoforms (NP_009168.4; NP_001005176.1; NP_001265380.1; NP_001265381.1; NP_001265382.1).
ISO Certification
Manufactured in an ISO 9001:2008 Certified Laboratory.
Applications Tested/Suitable for anti-SP140 antibody
Peptide ELISA (EIA), Western Blot (WB)
Application Notes for anti-SP140 antibody
Peptide ELISA: Antibody detection limit dilution 1: 128000.
Western Blot: Approx 140kDa band observed in Human Spleen lysates (calculated MW of 98.2kDa according to NP_009168.4). Recommended concentration: 1-3ug/ml.

Western Blot (WB) of anti-SP140 antibody

(2ug/ml) staining of Human Spleen lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Goat Anti-SP140 Polyclonal Antibody – Cat# MBS423403

Other Notes

Small volumes of anti-SP140 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for SP140. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Secondary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
nuclear body protein SP140 isoform 1
NCBI Official Synonym Full Names
SP140 nuclear body protein
NCBI Official Symbol
SP140
NCBI Official Synonym Symbols
LYSP100; LYSP100-A; LYSP100-B
UniProt Protein Name
Nuclear body protein SP140
UniProt Synonym Protein Names
Lymphoid-restricted homolog of Sp100
Protein Family
UniProt Gene Name
SP140
UniProt Synonym Gene Names
LYSp100
UniProt Entry Name
SP140_HUMAN
NCBI Protein Information
nuclear body protein SP140

UniProt Comments for SP140

SP140: Component of the nuclear body, also known as nuclear domain 10, PML oncogenic domain, and KR body. May be involved in the pathogenesis of acute promyelocytic leukemia and viral infection. 4 isoforms of the human protein are produced by alternative splicing.

Protein type: Transcription, coactivator/corepressor

Chromosomal Location of Human Ortholog: 2q37.1

Cellular Component: mitochondrion; nuclear envelope; nucleolus; nucleoplasm; nucleus; PML body

Molecular Function: DNA binding; protein binding; transcription factor activity; zinc ion binding

Biological Process: defense response; regulation of transcription, DNA-dependent
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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