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HS3ST1 Antibody – Goat Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS423432

MBS423432 is an antibody targeting HS3ST1. Also known as Goat anti-HS3ST1 Antibody. Reactive in Tested: Human; Expected from sequence similarity: Human, Cow. Host: Goat. More details

$450 / 0.1 mg +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
HS3ST1, Polyclonal Antibody
Full Product Name
Goat anti-HS3ST1 Antibody
Product Gene Name
Product Synonym Names
HS3ST1; heparan sulfate (glucosamine) 3-O-sulfotransferase 1; 3OST; 3OST1; 3-OST-1; h3-OST-1; heparan sulfate 3-O-sulfotransferase 1; heparan sulfate D-glucosaminyl 3-O-sulfotransferase 1; heparan sulfate glucosamine 3-O-sulfotransferase 1; heparin-glucos; HS3ST1 antibody; heparan sulfate (glucosamine) 3-O-sulfotransferase 1 antibody; 3OST antibody; 3OST1 antibody; 3-OST-1 antibody; h3-OST-1 antibody; heparan sulfate 3-O-sulfotransferase 1 antibody; heparan sulfate D-glucosaminyl 3-O-sulfotransferase 1 antibody; heparan sulfate glucosamine 3-O-sulfotransferase 1 antibody; heparin-glucos antibody
Immunogen Sequence
NKLHEYFHEP NKK
Clonality
Polyclonal
Host
Goat
Species Reactivity
Tested: Human; Expected from sequence similarity: Human, Cow
Form/Format
Supplied at 0.5 mg/ml in Tris saline, 0. 02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Purity/Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Concentration
100ug specific antibody in 200ul (lot specific)
Preparation and Storage
Aliquot and store at -20 degree C. Minimize freezing and thawing.
Antibody/Peptide Pairs
HS3ST1 peptide (MBS428528) is used for blocking the activity of HS3ST1 antibody (MBS423432)
Molecular Weight
35,773 Da
OMIM
603244
Immunogen
Peptide with sequence C-NKLHEYFHEPNKK, from the C Terminus of the protein sequence according to NP_005105.1.
Epitope
C Terminus
ISO Certification
Manufactured in an ISO 9001:2008 Certified Laboratory.
Applications Tested/Suitable for anti-HS3ST1 antibody
Peptide ELISA (EIA), Western Blot (WB)
Application Notes for anti-HS3ST1 antibody
Peptide ELISA: Antibody detection limit dilution 1: 128000.
Western Blot: Approx 35kDa band observed in Human Brain (Cerebellum, Cerebral Cortex, Frontal Cortex) lysates (calculated MW of 35.8kDa according to NP_005105.1). Recommended concentration: 1-3ug/ml.

Western Blot (WB) of anti-HS3ST1 antibody

(1ug/ml) staining of Human Cerebellum lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Goat Anti-HS3ST1 Polyclonal Antibody – Cat# MBS423432

Other Notes

Small volumes of anti-HS3ST1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for HS3ST1. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Secondary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
heparan sulfate glucosamine 3-O-sulfotransferase 1
NCBI Official Synonym Full Names
heparan sulfate-glucosamine 3-sulfotransferase 1
NCBI Official Symbol
HS3ST1
NCBI Official Synonym Symbols
3OST; 3OST1
UniProt Protein Name
Heparan sulfate glucosamine 3-O-sulfotransferase 1
UniProt Synonym Protein Names
Heparan sulfate D-glucosaminyl 3-O-sulfotransferase 1; 3-OST-1; Heparan sulfate 3-O-sulfotransferase 1; h3-OST-1
UniProt Gene Name
HS3ST1
UniProt Synonym Gene Names
3OST; 3OST1; 3-OST-1; Heparan sulfate 3-O-sulfotransferase 1; h3-OST-1
UniProt Entry Name
HS3S1_HUMAN
NCBI Protein Information
heparan sulfate glucosamine 3-O-sulfotransferase 1

NCBI Summary for HS3ST1

Heparan sulfate biosynthetic enzymes are key components in generating a myriad of distinct heparan sulfate fine structures that carry out multiple biologic activities. The enzyme encoded by this gene is a member of the heparan sulfate biosynthetic enzyme family. It possesses both heparan sulfate glucosaminyl 3-O-sulfotransferase activity, anticoagulant heparan sulfate conversion activity, and is a rate limiting enzyme for synthesis of anticoagulant heparan. This enzyme is an intraluminal Golgi resident protein. [provided by RefSeq, Jul 2008]
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UniProt Comments for HS3ST1

HS3ST1: Sulfotransferase that utilizes 3'-phospho-5'-adenylyl sulfate (PAPS) to catalyze the transfer of a sulfo group to position 3 of glucosamine residues in heparan. Catalyzes the rate limiting step in the biosynthesis of heparan sulfate (HSact). This modification is a crucial step in the biosynthesis of anticoagulant heparan sulfate as it completes the structure of the antithrombin pentasaccharide binding site. Belongs to the sulfotransferase 1 family.

Protein type: Glycan Metabolism - heparan sulfate biosynthesis; Transferase; EC 2.8.2.23

Chromosomal Location of Human Ortholog: 4p16

Cellular Component: Golgi lumen; integral to membrane

Molecular Function: heparin-glucosamine 3-O-sulfotransferase activity; sulfotransferase activity

Biological Process: glycosaminoglycan biosynthetic process
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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