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VPS16 Antibody – Goat Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS423449

MBS423449 is an antibody targeting VPS16. Also known as Goat anti-VPS16 (isoform 1) Antibody. Reactive in Tested: Human, Mouse, Rat; Expected from sequence similarity: Human, Mouse, Rat, Dog, Pig, Cow. Host: Goat. More details

$450 / 0.1 mg +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
VPS16, Polyclonal Antibody
Full Product Name
Goat anti-VPS16 (isoform 1) Antibody
Product Gene Name
Product Synonym Names
Goat Anti-VPS16 Antibody; VPS16; vacuolar protein sorting 16 homolog (S. cerevisiae); hVPS16; OTTHUMP00000030047; OTTHUMP00000030048; vacuolar protein sorting protein 16; vacuolar protein sorting-associated protein 16 homolog; VPS16 antibody; vacuolar protein sorting 16 homolog (S. cerevisiae) antibody; hVPS16 antibody; OTTHUMP00000030047 antibody; OTTHUMP00000030048 antibody; vacuolar protein sorting protein 16 antibody; vacuolar protein sorting-associated protein 16 homolog antibody; VPS16 (isoform 1)
Immunogen Sequence
KESQKADEYL REIQ
Clonality
Polyclonal
Host
Goat
Species Reactivity
Tested: Human, Mouse, Rat; Expected from sequence similarity: Human, Mouse, Rat, Dog, Pig, Cow
Form/Format
Supplied at 0.5 mg/ml in Tris saline, 0. 02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Purity/Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Concentration
100ug specific antibody in 200ul (lot specific)
Preparation and Storage
Aliquot and store at -20 degree C. Minimize freezing and thawing.
Antibody/Peptide Pairs
VPS16 peptide (MBS428415) is used for blocking the activity of VPS16 antibody (MBS423449)
Molecular Weight
78,300 Da
OMIM
608550
Immunogen
Peptide with sequence C-KESQKADEYLREIQ, from the internal region of the protein sequence according to NP_072097.2.
Epitope
Internal region
Note
This antibody is expected to recognize reported isoform 1 (NP_072097.2) only.
ISO Certification
Manufactured in an ISO 9001:2008 Certified Laboratory.
Applications Tested/Suitable for anti-VPS16 antibody
Peptide ELISA (EIA), Western Blot (WB)
Application Notes for anti-VPS16 antibody
Peptide ELISA: Antibody detection limit dilution 1: 128000.
Western Blot: Approx 100kDa band observed in lysates of cell lines HepG2, HeLa and Jurkat and in lysates of Mouse Spleen and Rat Thymus (calculated MW of 94.7kDa according to NP_072097.2). Recommended concentration: 0.1-0.3ug/ml.

Western Blot (WB) of anti-VPS16 antibody

(0.1ug/ml) staining of HeLa (A), HepG2 (B) and Jurkat (C) lysates (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Goat Anti-VPS16 Polyclonal Antibody – Cat# MBS423449

Western Blot (WB) of anti-VPS16 antibody

(0.1ug/ml) staining of Mouse Spleen lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Goat Anti-VPS16 Polyclonal Antibody, Western Blot (WB) – Cat# MBS423449

Other Notes

Small volumes of anti-VPS16 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for VPS16. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Secondary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
vacuolar protein sorting-associated protein 16 homolog isoform 1
NCBI Official Synonym Full Names
VPS16, CORVET/HOPS core subunit
NCBI Official Symbol
VPS16
NCBI Official Synonym Symbols
hVPS16
UniProt Protein Name
Vacuolar protein sorting-associated protein 16 homolog
UniProt Gene Name
VPS16
UniProt Synonym Gene Names
hVPS16
UniProt Entry Name
VPS16_HUMAN
NCBI Protein Information
vacuolar protein sorting-associated protein 16 homolog

NCBI Summary for VPS16

Vesicle mediated protein sorting plays an important role in segregation of intracellular molecules into distinct organelles. Genetic studies in yeast have identified more than 40 vacuolar protein sorting (VPS) genes involved in vesicle transport to vacuoles. This gene encodes the human homolog of yeast class C Vps16 protein. The mammalian class C Vps proteins are predominantly associated with late endosomes/lysosomes, and like their yeast counterparts, may mediate vesicle trafficking steps in the endosome/lysosome pathway. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2009]
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UniProt Comments for VPS16

VPS16: May play a role in vesicle-mediated protein trafficking to lysosomal compartments and in membrane docking/fusion reactions of late endosomes/lysosomes. Belongs to the VPS16 family. 2 isoforms of the human protein are produced by alternative splicing.

Chromosomal Location of Human Ortholog: 20p13

Cellular Component: autophagic vacuole; axon; cell soma; clathrin-coated vesicle; early endosome; late endosome; late endosome membrane; lysosomal membrane; lysosome; recycling endosome

Molecular Function: actin binding; actin filament binding; protein binding

Biological Process: endosome to lysosome transport; intracellular protein transport; regulation of vacuole fusion, non-autophagic
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Diseases associated with anti-VPS16 antibody

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