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NR5A2 Antibody – Goat Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS423452

MBS423452 is an antibody targeting NR5A2. Also known as Goat anti-NR5A2/LRH1 Antibody. Reactive in Tested: Human, Pig; Expected from sequence similarity: Human, Mouse, Rat, Dog, Pig, Cow. Host: Goat. More details

$450 / 0.1 mg +1 FREE 8GB USB
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Testing Data View PDF
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Product Overview

Online Datasheet in PDF
Product Name
NR5A2/LRH1, Polyclonal Antibody
Full Product Name
Goat anti-NR5A2/LRH1 Antibody
Product Gene Name
anti-NR5A2 antibody
Product Synonym Gene Name
LRH1
Product Synonym Names
Goat Anti-NR5A2 / LRH1 (aa328-338) Antibody; NR5A2; nuclear receptor subfamily 5, group A, member 2; B1F; B1F2; CPF; FTF; FTZ-F1; FTZ-F1beta; LRH-1; LRH1; hB1F-2; CYP7A promoter-binding factor; alpha-1-fetoprotein transcription factor; b1-binding factor, hepatocyte transcription factor which activat; NR5A2 antibody; nuclear receptor subfamily 5; group A; member 2 antibody; B1F antibody; B1F2 antibody; CPF antibody; FTF antibody; FTZ-F1 antibody; FTZ-F1beta antibody; LRH-1 antibody; LRH1 antibody; hB1F-2 antibody; CYP7A promoter-binding factor antibody; alpha-1-fetoprotein transcription factor antibody; b1-binding factor; hepatocyte transcription factor which activates enhancer II of hepatitis B virus antibody; fetoprotein-alpha 1 (AFP) transcription factor antibody; hepatocytic transcription factor antibody; liver nuclear receptor homolog-1 variant 2 antibody; liver receptor homolog 1 antibody; liver receptor homolog-1 antibody; nuclear receptor NR5A2 antibody; nuclear receptor subfamily 5 group A member 2 antibody; NR5A2 / LRH1
Immunogen Sequence
TDYDRSPFVT SPIS
Clonality
Polyclonal
Host
Goat
Species Reactivity
Tested: Human, Pig; Expected from sequence similarity: Human, Mouse, Rat, Dog, Pig, Cow
Form/Format
Supplied at 0.5 mg/ml in Tris saline, 0. 02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Purity/Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Concentration
100ug specific antibody in 200ul (lot specific)
Preparation and Storage
Aliquot and store at -20 degree C. Minimize freezing and thawing.
Antibody/Peptide Pairs
NR5A2 / LRH1 peptide (MBS428038) is used for blocking the activity of NR5A2/LRH1 antibody (MBS423452)
Molecular Weight
53,657 Da
OMIM
604453
Immunogen
Peptide with sequence C-TDYDRSPFVTSPIS, from the internal region of the protein sequence according to NP_995582.1; NP_003813.1.
Epitope
Internal region
Note
This antibody is expected to recognize both reported isoforms (NP_995582.1; NP_003813.1).
ISO Certification
Manufactured in an ISO 9001:2008 Certified Laboratory.
Applications Tested/Suitable for anti-NR5A2 antibody
Peptide ELISA (EIA), Western Blot (WB)
Application Notes for anti-NR5A2 antibody
Peptide ELISA: Antibody detection limit dilution 1: 128000.
Western Blot: Approx 55kDa band observed in nuclear lysates of cell lines HeLa and HepG2, and approx 60kDa band observed in Pig Ovary lysates (calculated MW of 56.5kDa according to Human NP_003813.1 and Pig AFD98842.1). Recommended concentration: 0.3-1ug/ml.

Western Blot (WB) of anti-NR5A2 antibody

(1ug/ml) staining of HepG2 nuclear lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Goat Anti-NR5A2 Polyclonal Antibody – Cat# MBS423452

Western Blot (WB) of anti-NR5A2 antibody

(0.3ug/ml) staining of Pig Ovary lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Goat Anti-NR5A2 Polyclonal Antibody, Western Blot (WB) – Cat# MBS423452

Other Notes

Small volumes of anti-NR5A2 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for NR5A2. It may not necessarily be applicable to this product.

NCBI GI #
45545405
NCBI GeneID
2494
NCBI Accession #
NP_995582.1
NCBI Related Accession #
NP_003813.1
UniProt Secondary Accession #
O95642; Q147U3; B4E2P3
UniProt Related Accession #
O00482
GenBank Nucleotide #
NM_205860.2
NCBI Official Full Name
nuclear receptor subfamily 5 group A member 2 isoform 1
NCBI Official Synonym Full Names
nuclear receptor subfamily 5 group A member 2
NCBI Official Symbol
NR5A2
NCBI Official Synonym Symbols
B1F; CPF; FTF; B1F2; LRH1; LRH-1; FTZ-F1; hB1F-2; FTZ-F1beta
UniProt Protein Name
Nuclear receptor subfamily 5 group A member 2
UniProt Synonym Protein Names
Alpha-1-fetoprotein transcription factor; B1-binding factor; hB1F; CYP7A promoter-binding factor; Hepatocytic transcription factor; Liver receptor homolog 1; LRH-1
Protein Family
Nuclear receptor subfamily
UniProt Gene Name
NR5A2
UniProt Synonym Gene Names
B1F; CPF; FTF; hB1F; LRH-1
UniProt Entry Name
NR5A2_HUMAN
NCBI Protein Information
nuclear receptor subfamily 5 group A member 2

UniProt Comments for NR5A2

NR5A2: Binds to the sequence element 5'-AACGACCGACCTTGAG-3' of the enhancer II of hepatitis B virus genes, a critical cis-element of their expression and regulation. May be responsible for the liver-specific activity of enhancer II, probably in combination with other hepatocyte transcription factors. Key regulator of cholesterol 7-alpha-hydroxylase gene (CYP7A) expression in liver. May also contribute to the regulation of pancreas-specific genes and play important roles in embryonic development. Belongs to the nuclear hormone receptor family. NR5 subfamily. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Nuclear receptor; Transcription factor; DNA-binding

Chromosomal Location of Human Ortholog: 1q32.1

Cellular Component: cytoplasm; nucleoplasm; nucleus

Molecular Function: chromatin binding; DNA binding; ligand-dependent nuclear receptor activity; phospholipid binding; protein binding; RNA polymerase II transcription factor activity, enhancer binding; sequence-specific DNA binding; steroid hormone receptor activity; transcription factor activity; zinc ion binding

Biological Process: bile acid metabolic process; cholesterol homeostasis; embryonic development; epithelial cell differentiation; homeostatic process; hormone-mediated signaling; intracellular receptor-mediated signaling pathway; positive regulation of transcription from RNA polymerase II promoter; positive regulation of transcription, DNA-dependent; positive regulation of viral genome replication; regulation of cell proliferation; regulation of transcription, DNA-dependent; steroid hormone mediated signaling; tissue development; transcription initiation from RNA polymerase II promoter
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Research Articles on NR5A2

1. Results identify LRH-1 as a critical component of the anti-inflammatory and fungicidal response of alternatively activated macrophages that acts upstream from the IL-13-induced 15-HETE/PPARgamma axis.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-NR5A2 antibody

 Reference Product  PubMed Publications
 CYP19A1 antibody  >45 publications with NR5A2 and CYP19A1
 STAR antibody  >17 publications with NR5A2 and STAR
 CYP7A1 antibody  >15 publications with NR5A2 and CYP7A1
 CTNNB1 antibody  >14 publications with NR5A2 and CTNNB1
 NR0B2 antibody  >9 publications with NR5A2 and NR0B2
 HSD3B2 antibody  >7 publications with NR5A2 and HSD3B2
 ABCB11 antibody  >2 publications with NR5A2 and ABCB11
 GPS2 antibody  >1 publications with NR5A2 and GPS2
 NCOA3 antibody  >1 publications with NR5A2 and NCOA3
 EDF1 antibody  >1 publications with NR5A2 and EDF1

Pathways associated with anti-NR5A2 antibody

 Products by Pathway  Pathway Diagram
 Developmental Biology Pathway antibodies  Developmental Biology Pathway Diagram
 Gene Expression Pathway antibodies  Gene Expression Pathway Diagram
 Generic Transcription Pathway antibodies  Generic Transcription Pathway Diagram
 Maturity Onset Diabetes Of The Young Pathway antibodies  Maturity Onset Diabetes Of The Young Pathway Diagram
 Maturity Onset Diabetes Of The Young Pathway antibodies  Maturity Onset Diabetes Of The Young Pathway Diagram
 Nuclear Receptor Transcription Pathway antibodies  Nuclear Receptor Transcription Pathway Diagram
 Nuclear Receptors Pathway antibodies  Nuclear Receptors Pathway Diagram
 Regulation Of Beta-cell Development Pathway antibodies  Regulation Of Beta-cell Development Pathway Diagram
 Regulation Of Gene Expression In Early Pancreatic Precursor Cells Pathway antibodies  Regulation Of Gene Expression In Early Pancreatic Precursor Cells Pathway Diagram

Diseases associated with anti-NR5A2 antibody

 Disease Name  Pubmed Publications
 Liver Neoplasms Antibodies  >41 publications with NR5A2 and Liver Neoplasms
 Liver Diseases Antibodies  >35 publications with NR5A2 and Liver Diseases
 Adenocarcinoma Antibodies  >22 publications with NR5A2 and Adenocarcinoma
 Breast Neoplasms Antibodies  >20 publications with NR5A2 and Breast Neoplasms
 Pancreatic Neoplasms Antibodies  >17 publications with NR5A2 and Pancreatic Neoplasms
 Inflammation Antibodies  >14 publications with NR5A2 and Inflammation
 Fatty Liver Antibodies  >13 publications with NR5A2 and Fatty Liver
 Infertility, Female Antibodies  >10 publications with NR5A2 and Infertility, Female
 Neoplasms, Experimental Antibodies  >7 publications with NR5A2 and Neoplasms, Experimental
 Carcinoma, Hepatocellular Antibodies  >7 publications with NR5A2 and Carcinoma, Hepatocellular

Organs/Tissues associated with anti-NR5A2 antibody

 Organ/Tissue Name  Pubmed Publications
 Liver Antibodies  >226 publications with NR5A2 and Liver
 Pancreas Antibodies  >26 publications with NR5A2 and Pancreas
 Intestine Antibodies  >25 publications with NR5A2 and Intestine
 Testis Antibodies  >24 publications with NR5A2 and Testis
 Embryonic Tissue Antibodies  >21 publications with NR5A2 and Embryonic Tissue
 Brain Antibodies  >20 publications with NR5A2 and Brain
 Adrenal Gland Antibodies  >13 publications with NR5A2 and Adrenal Gland
 Kidney Antibodies  >6 publications with NR5A2 and Kidney
 Lung Antibodies  >6 publications with NR5A2 and Lung
 Muscle Antibodies  >6 publications with NR5A2 and Muscle
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