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MARCH7 Antibody – Goat Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS423476

MBS423476 is an antibody targeting MARCH7. Also known as Goat anti-MARCH7/Axotrophin Antibody. Reactive in Tested: Mouse; Expected from sequence similarity: Human, Mouse, Rat. Host: Goat. More details

$450 / 0.1 mg +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
MARCH7/Axotrophin, Polyclonal Antibody
Full Product Name
Goat anti-MARCH7/Axotrophin Antibody
Product Gene Name
Product Synonym Names
Goat Anti-MARCH-VII / Axotrophin Antibody; MARCH7; membrane-associated ring finger (C3HC4) 7; AXO; AXOT; DKFZP586F1122; MARCH-VII; RNF177; axotrophin; membrane-associated RING-CH protein VII; MARCH7 antibody; membrane-associated ring finger (C3HC4) 7 antibody; AXO antibody; AXOT antibody; DKFZP586F1122 antibody; MARCH-VII antibody; RNF177 antibody; axotrophin antibody; membrane-associated RING-CH protein VII antibody; Axotrophin; MARCH7 / Axotrophin
Immunogen Sequence
RTLQAHMEDL ETSED
Clonality
Polyclonal
Host
Goat
Species Reactivity
Tested: Mouse; Expected from sequence similarity: Human, Mouse, Rat
Form/Format
Supplied at 0.5 mg/ml in Tris saline, 0. 02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Purity/Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Concentration
100ug specific antibody in 200ul (lot specific)
Preparation and Storage
Aliquot and store at -20 degree C. Minimize freezing and thawing.
Antibody/Peptide Pairs
Molecular Weight
73,778 Da
OMIM
613334
Immunogen
Peptide with sequence C-RTLQAHMEDLETSED, from the internal region of the protein sequence according to NP_073737.1.
Epitope
Internal region
ISO Certification
Manufactured in an ISO 9001:2008 Certified Laboratory.
Applications Tested/Suitable for anti-MARCH7 antibody
Peptide ELISA (EIA), Western Blot (WB)
Application Notes for anti-MARCH7 antibody
Peptide ELISA: Antibody detection limit dilution 1: 16000.
Western Blot: Approx 75kDa band observed in Mouse Skeletal Muscle lysates (calculated MW of 76.6kDa according to NP_065600.1. Recommended concentration: 1-3ug/ml.

Western Blot (WB) of anti-MARCH7 antibody

((2ug/ml) staining of Mouse Skeletal Muscle lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Goat Anti-MARCH7 Polyclonal Antibody – Cat# MBS423476

Other Notes

Small volumes of anti-MARCH7 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for MARCH7. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Secondary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
E3 ubiquitin-protein ligase MARCH7 isoform a
NCBI Official Synonym Full Names
membrane associated ring-CH-type finger 7
NCBI Official Symbol
MARCH7
NCBI Official Synonym Symbols
AXO; AXOT; RNF177; MARCH-VII
UniProt Protein Name
E3 ubiquitin-protein ligase MARCH7
UniProt Synonym Protein Names
Axotrophin; Membrane-associated RING finger protein 7; Membrane-associated RING-CH protein VII; MARCH-VII; RING finger protein 177
UniProt Gene Name
MARCH7
UniProt Synonym Gene Names
AXOT; RNF177; MARCH-VII
UniProt Entry Name
MARH7_HUMAN
NCBI Protein Information
E3 ubiquitin-protein ligase MARCH7

NCBI Summary for MARCH7

MARCH7 is a member of the MARCH family of membrane-bound E3 ubiquitin ligases (EC 6.3.2.19). MARCH proteins add ubiquitin (see MIM 191339) to target lysines in substrate proteins, thereby signaling their vesicular transport between membrane compartments (Bartee et al., 2004 [PubMed 14722266]).[supplied by OMIM, Mar 2010]
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UniProt Comments for MARCH7

MARCH7: E3 ubiquitin-protein ligase which may specifically enhance the E2 activity of HIP2. E3 ubiquitin ligases accept ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfer the ubiquitin to targeted substrates.

Protein type: EC 6.3.2.-; Ubiquitin ligase; EC 6.3.2.19; Ubiquitin conjugating system; Ligase

Chromosomal Location of Human Ortholog: 2q24.2

Molecular Function: ligase activity; zinc ion binding

Biological Process: negative regulation of T cell proliferation; protein ubiquitination; regulation of tolerance induction
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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