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IL3RA Antibody – Goat Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS423523

MBS423523 is an antibody targeting IL3RA. Also known as Goat anti-IL3RA/CD123 Antibody. Reactive in Tested: Human; Expected from sequence similarity: Human. Host: Goat. More details

$450 / 0.1 mg +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
IL3RA/CD123, Polyclonal Antibody
Full Product Name
Goat anti-IL3RA/CD123 Antibody
Product Gene Name
Product Synonym Gene Name
CD123
Product Synonym Names
IL3RA; interleukin 3 receptor, alpha (low affinity); CD123; IL3R; IL3RAY; IL3RX; IL3RY; hIL-3Ra; CD123 antigen; IL-3 receptor alpha SP2 isoform; IL-3 receptor subunit alpha; IL-3R subunit alpha; IL-3R-alpha; IL-3RA; interleukin-3 receptor subunit alpha; IL3RA antibody; interleukin 3 receptor; alpha (low affinity) antibody; CD123 antibody; IL3R antibody; IL3RAY antibody; IL3RX antibody; IL3RY antibody; hIL-3Ra antibody; CD123 antigen antibody; IL-3 receptor alpha SP2 isoform antibody; IL-3 receptor subunit alpha antibody; IL-3R subunit alpha antibody; IL-3R-alpha antibody; IL-3RA antibody; interleukin-3 receptor subunit alpha antibody; IL3RA / CD123
Immunogen Sequence
RQQYECLHYK TD
Clonality
Polyclonal
Host
Goat
Species Reactivity
Tested: Human; Expected from sequence similarity: Human
Form/Format
Supplied at 0.5 mg/ml in Tris saline, 0. 02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Purity/Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Concentration
100ug specific antibody in 200ul (lot specific)
Preparation and Storage
Aliquot and store at -20 degree C. Minimize freezing and thawing.
Antibody/Peptide Pairs
Molecular Weight
34,466 Da
OMIM
308385
Immunogen
Peptide with sequence RQQYECLHYKTD, from the internal region of the protein sequence according to NP_002174.1; NP_001254642.1.
Epitope
Internal region
Note
This antibody is expected to recognize both reported isoforms (NP_002174.1; NP_001254642.1).
ISO Certification
Manufactured in an ISO 9001:2008 Certified Laboratory.
Applications Tested/Suitable for anti-IL3RA antibody
Peptide ELISA (EIA), Western Blot (WB)
Application Notes for anti-IL3RA antibody
Peptide ELISA: Antibody detection limit dilution 1: 128000.
Western Blot: Approx 43kDa band observed in lysates of cell lines Jurkat and Molt4 (calculated MW of 43.3kDa according to NP_002174.1). Recommended concentration: 1-3ug/ml.

Western Blot (WB) of anti-IL3RA antibody

(2ug/ml) staining of Jurkat (A) and Molt4 (B) lysates (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Goat Anti-IL3RA Polyclonal Antibody – Cat# MBS423523

Other Notes

Small volumes of anti-IL3RA antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for IL3RA. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI Related Accession #
UniProt Secondary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
interleukin-3 receptor subunit alpha isoform 1
NCBI Official Synonym Full Names
interleukin 3 receptor subunit alpha
NCBI Official Symbol
IL3RA
NCBI Official Synonym Symbols
IL3R; CD123; IL3RX; IL3RY; IL3RAY; hIL-3Ra
UniProt Protein Name
Interleukin-3 receptor subunit alpha
UniProt Synonym Protein Names
CD_antigen: CD123
UniProt Gene Name
IL3RA
UniProt Synonym Gene Names
IL3R; IL-3 receptor subunit alpha; IL-3R subunit alpha; IL-3R-alpha; IL-3RA
UniProt Entry Name
IL3RA_HUMAN
NCBI Protein Information
interleukin-3 receptor subunit alpha

NCBI Summary for IL3RA

The protein encoded by this gene is an interleukin 3 specific subunit of a heterodimeric cytokine receptor. The receptor is comprised of a ligand specific alpha subunit and a signal transducing beta subunit shared by the receptors for interleukin 3 (IL3), colony stimulating factor 2 (CSF2/GM-CSF), and interleukin 5 (IL5). The binding of this protein to IL3 depends on the beta subunit. The beta subunit is activated by the ligand binding, and is required for the biological activities of IL3. This gene and the gene encoding the colony stimulating factor 2 receptor alpha chain (CSF2RA) form a cytokine receptor gene cluster in a X-Y pseudoautosomal region on chromosomes X or Y. Alternatively spliced transcript variants encoding distinct isoforms have been found. [provided by RefSeq, Jun 2012]
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UniProt Comments for IL3RA

IL3RA: This is a receptor for interleukin-3. Belongs to the type I cytokine receptor family. Type 5 subfamily. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral

Chromosomal Location of Human Ortholog: Xp22.3 or Yp11.3

Cellular Component: integral to membrane; intracellular; plasma membrane

Molecular Function: interleukin-3 receptor activity; protein-tyrosine kinase activity; Ras guanyl-nucleotide exchange factor activity

Biological Process: MAPKKK cascade; peptidyl-tyrosine phosphorylation; positive regulation of GTPase activity
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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