Goat Anti-TPI1 Polyclonal Antibody – Cat# MBS423550

Biotinylated (0. 03ug/ml) staining of Human Liver lysate (35ug protein in RIPA buffer), exactly mirroring its parental non-biotinylated product. Primary incubation was 1 hour. Detected by chemiluminescence, using streptavidin-HRP and using NAP blocker as a substitute for skimmed milk.

Triosephosphate Isomerase (TPI1) Antibody – Goat Polyclonal

Name: TPI1 Antibody; Polyclonal; Goat; Human/Mouse-Reactive; MyBioSource
Brand: MyBioSource
SKU: MBS423550
Price: 450 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS423550

MBS423550 is an antibody targeting TPI1. Also known as Goat anti-Triosephosphate isomerase, Biotinylated Antibody. Reactive in Tested: Human, Mouse; Expected from sequence similarity: Human, Mouse, Rat, Dog, Cow. Host: Goat. More details

$450 / 0.1 mg +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Online Datasheet in PDF

Product Name

Triosephosphate isomerase (TPI1), Polyclonal Antibody

Full Product Name

Goat anti-Triosephosphate isomerase, Biotinylated Antibody

Product Gene Name

anti-TPI1 antibody

Product Synonym Names

Goat Anti-Triosephosphate isomerase Antibody; TPI1; triosephosphate isomerase 1; HEL-S-49; TIM; TPI; TPID; epididymis secretory protein Li 49; triose-phosphate isomerase; TPI1 antibody; triosephosphate isomerase 1 antibody; HEL-S-49 antibody; TIM antibody; TPI antibody; TPID antibody; epididymis secretory protein Li 49 antibody; triose-phosphate isomerase antibody; Triosephosphate isomerase; Triosephosphate isomerase, Biotinylated

Immunogen Sequence

LKPEFVDIIN AKQ

Clonality

Polyclonal

Host

Goat

Species Reactivity

Tested: Human, Mouse; Expected from sequence similarity: Human, Mouse, Rat, Dog, Cow

Form/Format

Supplied at 0.5 mg/ml in Tris saline, 0. 02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

Purity/Purification

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Concentration

100ug specific antibody in 200ul (lot specific)

Preparation and Storage

Aliquot and store at -20 degree C. Minimize freezing and thawing.

Matching Pairs

Unconjugated Antibody: Triosephosphate isomerase (MBS420245)
Biotin Conjugated Antibody: Triosephosphate isomerase (MBS423550)

Antibody/Peptide Pairs

Triosephosphate isomerase peptide (MBS426214) is used for blocking the activity of Triosephosphate isomerase antibody (MBS423550)

Molecular Weight

17,958 Da

OMIM

190450

Immunogen

Peptide with sequence C-LKPEFVDIINAKQ. , from the C Terminus of the protein sequence according to NP_000356.1; NP_001152759.1.

Epitope

C Terminus

Note

This antibody is expected to recognise reported isoforms 1 and 2 (NP_000356.1; NP_001152759.1).

ISO Certification

Manufactured in an ISO 9001:2008 Certified Laboratory.

Applications Tested/Suitable for anti-TPI1 antibody

Peptide ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC)

Application Notes for anti-TPI1 antibody

Peptide ELISA: Antibody detection limit dilution 1: 16000.
Western Blot: Approx 26kDa band observed in Human Liver lysates (calculated MW of 26.7kDa according to NP_000356.1). See non-biotinylated parental product's datasheet for further QC data. Recommended concentration: 0.01-0.03ug/ml.

Other Notes

Small volumes of anti-TPI1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for TPI1. It may not necessarily be applicable to this product.

NCBI GI #

4507645

NCBI GeneID

7167

NCBI Accession #

NP_000356.1

NCBI Related Accession #

NP_001152759.1

UniProt Secondary Accession #

P00938; Q6FHP9; Q6IS07; Q8WWD0; Q96AG5; B7Z5D8; D3DUS9

UniProt Related Accession #

P60174

GenBank Nucleotide #

NM_000365.5

NCBI Official Full Name

triosephosphate isomerase isoform 1

NCBI Official Synonym Full Names

triosephosphate isomerase 1

NCBI Official Symbol

TPI1

NCBI Official Synonym Symbols

TIM; TPI; TPID; HEL-S-49

UniProt Protein Name

Triosephosphate isomerase

UniProt Synonym Protein Names

Triose-phosphate isomerase

Protein Family

Triosephosphate isomerase

UniProt Gene Name

TPI1

UniProt Synonym Gene Names

TPI; TIM

UniProt Entry Name

TPIS_HUMAN

NCBI Protein Information

triosephosphate isomerase

NCBI Summary for TPI1

This gene encodes an enzyme, consisting of two identical proteins, which catalyzes the isomerization of glyceraldehydes 3-phosphate (G3P) and dihydroxy-acetone phosphate (DHAP) in glycolysis and gluconeogenesis. Mutations in this gene are associated with triosephosphate isomerase deficiency. Pseudogenes have been identified on chromosomes 1, 4, 6 and 7. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Apr 2009]

UniProt Comments for TPI1

TPI1: Defects in TPI1 are the cause of triosephosphate isomerase deficiency (TPI deficiency). TPI deficiency is an autosomal recessive disorder. It is the most severe clinical disorder of glycolysis. It is associated with neonatal jaundice, chronic hemolytic anemia, progressive neuromuscular dysfunction, cardiomyopathy and increased susceptibility to infection. Belongs to the triosephosphate isomerase family. 2 isoforms of the human protein are produced by alternative promoter.

Protein type: Isomerase; Carbohydrate Metabolism - fructose and mannose; Carbohydrate Metabolism - glycolysis and gluconeogenesis; Carbohydrate Metabolism - inositol phosphate; EC 5.3.1.1

Chromosomal Location of Human Ortholog: 12p13

Cellular Component: cytosol; extracellular space; nucleus

Molecular Function: protein binding; triose-phosphate isomerase activity; ubiquitin protein ligase binding

Biological Process: gluconeogenesis; glyceraldehyde-3-phosphate biosynthetic process; glycerol catabolic process; glycolysis; multicellular organismal development; pentose-phosphate shunt

Disease: Triosephosphate Isomerase Deficiency

Research Articles on TPI1

1. Polyvinylpyrrolidone stabilised silver nanoparticles (60 nM; 2-6 nm diameter) selectively inhibited PfTIM with a 7-fold decrease in enzyme catalytic efficiency (K(cat)/K(m)) over hTIM.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Pathways associated with anti-TPI1 antibody

Products by Pathway	Pathway Diagram
Biosynthesis Of Amino Acids Pathway antibodies	Biosynthesis Of Amino Acids Pathway Diagram
Biosynthesis Of Amino Acids Pathway antibodies	Biosynthesis Of Amino Acids Pathway Diagram
Carbon Metabolism Pathway antibodies	Carbon Metabolism Pathway Diagram
Carbon Metabolism Pathway antibodies	Carbon Metabolism Pathway Diagram
Fatty Acid Beta Oxidation Pathway antibodies	Fatty Acid Beta Oxidation Pathway Diagram
Fructose And Mannose Metabolism Pathway antibodies	Fructose And Mannose Metabolism Pathway Diagram
Fructose And Mannose Metabolism Pathway antibodies	Fructose And Mannose Metabolism Pathway Diagram
Gluconeogenesis Pathway antibodies	Gluconeogenesis Pathway Diagram
Gluconeogenesis, Oxaloacetate => Fructose-6P Pathway antibodies	Gluconeogenesis, Oxaloacetate => Fructose-6P Pathway Diagram
Gluconeogenesis, Oxaloacetate => Fructose-6P Pathway antibodies	Gluconeogenesis, Oxaloacetate => Fructose-6P Pathway Diagram

Diseases associated with anti-TPI1 antibody

Disease Name	Pubmed Publications
Disease Models, Animal Antibodies	>5 publications with TPI1 and Disease Models, Animal
Breast Neoplasms Antibodies	>3 publications with TPI1 and Breast Neoplasms
Hypertension Antibodies	>2 publications with TPI1 and Hypertension
Fibrosis Antibodies	>2 publications with TPI1 and Fibrosis
Anemia, Hemolytic Antibodies	>2 publications with TPI1 and Anemia, Hemolytic
Abnormalities, Drug-Induced Antibodies	>1 publications with TPI1 and Abnormalities, Drug-Induced
Nervous System Diseases Antibodies	>1 publications with TPI1 and Nervous System Diseases
Weight Loss Antibodies	>1 publications with TPI1 and Weight Loss
Liver Diseases Antibodies	>1 publications with TPI1 and Liver Diseases
Kidney Diseases Antibodies	>1 publications with TPI1 and Kidney Diseases

Organs/Tissues associated with anti-TPI1 antibody

Organ/Tissue Name	Pubmed Publications
Brain Antibodies	>7 publications with TPI1 and Brain
Blood Antibodies	>6 publications with TPI1 and Blood
Muscle Antibodies	>5 publications with TPI1 and Muscle
Kidney Antibodies	>3 publications with TPI1 and Kidney
Eye Antibodies	>3 publications with TPI1 and Eye
Prostate Antibodies	>2 publications with TPI1 and Prostate
Liver Antibodies	>2 publications with TPI1 and Liver
Mammary Gland Antibodies	>1 publications with TPI1 and Mammary Gland
Testis Antibodies	>1 publications with TPI1 and Testis
Esophagus Antibodies	>1 publications with TPI1 and Esophagus