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CPN10/HSPE1 (HSPE1) Antibody – Goat Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS423579

MBS423579 is an antibody targeting HSPE1. Also known as Goat anti-CPN10/HSPE1 Antibody. Reactive in Tested: Human; Expected from sequence similarity: Human, Mouse, Rat, Dog, Pig, Cow. Host: Goat. More details

$450 / 0.1 mg +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
CPN10/HSPE1, Polyclonal Antibody
Full Product Name
Goat anti-CPN10/HSPE1 Antibody
Product Gene Name
Product Synonym Gene Name
CPN10
Product Synonym Names
Goat Anti-CPN10 / HSPE1 (aa74-86) Antibody; HSPE1; heat shock 10kDa protein 1; CPN10; EPF; GROES; HSP10; 10 kDa chaperonin; 10 kDa heat shock protein, mitochondrial; chaperonin 10; early-pregnancy factor; heat shock 10kD protein 1 (chaperonin 10); HSPE1 antibody; heat shock 10kDa protein 1 antibody; CPN10 antibody; EPF antibody; GROES antibody; HSP10 antibody; 10 kDa chaperonin antibody; 10 kDa heat shock protein; mitochondrial antibody; chaperonin 10 antibody; early-pregnancy factor antibody; heat shock 10kD protein 1 (chaperonin 10) antibody; CPN10 / HSPE1
Immunogen Sequence
PEKSQGKVLQ AT
Clonality
Polyclonal
Host
Goat
Species Reactivity
Tested: Human; Expected from sequence similarity: Human, Mouse, Rat, Dog, Pig, Cow
Form/Format
Supplied at 0.5 mg/ml in Tris saline, 0. 02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Purity/Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Concentration
100ug specific antibody in 200ul (lot specific)
Preparation and Storage
Aliquot and store at -20 degree C. Minimize freezing and thawing.
Antibody/Peptide Pairs
Molecular Weight
10,932 Da
OMIM
600141
Immunogen
Peptide with sequence C-PEKSQGKVLQAT, from the internal region of the protein sequence according to NP_002148.1.
Epitope
Internal region
ISO Certification
Manufactured in an ISO 9001:2008 Certified Laboratory.
Applications Tested/Suitable for anti-HSPE1 antibody
Peptide ELISA (EIA), Western Blot (WB)
Application Notes for anti-HSPE1 antibody
Peptide ELISA: Antibody detection limit dilution 1: 128000.
Western Blot: Approx 10kDa band observed in lysates of cell line HepG2 (calculated MW of 10.9kDa according to NP_002148.1). Recommended concentration: 0.01-0.03ug/ml.

Western Blot (WB) of anti-HSPE1 antibody

(0. 01ug/ml) staining of HepG2 lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Goat Anti-HSPE1 Polyclonal Antibody – Cat# MBS423579

Other Notes

Small volumes of anti-HSPE1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for HSPE1. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Secondary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
10 kDa heat shock protein, mitochondrial
NCBI Official Synonym Full Names
heat shock protein family E (Hsp10) member 1
NCBI Official Symbol
HSPE1
NCBI Official Synonym Symbols
EPF; CPN10; GROES; HSP10
UniProt Protein Name
10 kDa heat shock protein, mitochondrial
UniProt Synonym Protein Names
10 kDa chaperonin; Chaperonin 10; CPN10; Early-pregnancy factor; EPF
UniProt Gene Name
HSPE1
UniProt Synonym Gene Names
Hsp10; CPN10; EPF
UniProt Entry Name
CH10_HUMAN
NCBI Protein Information
10 kDa heat shock protein, mitochondrial

NCBI Summary for HSPE1

This gene encodes a major heat shock protein which functions as a chaperonin. Its structure consists of a heptameric ring which binds to another heat shock protein in order to form a symmetric, functional heterodimer which enhances protein folding in an ATP-dependent manner. This gene and its co-chaperonin, HSPD1, are arranged in a head-to-head orientation on chromosome 2. Naturally occurring read-through transcription occurs between this locus and the neighboring locus MOBKL3.[provided by RefSeq, Feb 2011]
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UniProt Comments for HSPE1

HSPE1: Eukaryotic CPN10 homolog which is essential for mitochondrial protein biogenesis, together with CPN60. Binds to CPN60 in the presence of Mg-ATP and suppresses the ATPase activity of the latter. Belongs to the GroES chaperonin family.

Protein type: Mitochondrial

Chromosomal Location of Human Ortholog: 2q33.1

Cellular Component: membrane; mitochondrial matrix; mitochondrion

Molecular Function: ATP binding; chaperone binding; metal ion binding; protein binding; unfolded protein binding

Biological Process: caspase activation; chaperone cofactor-dependent protein folding; osteoblast differentiation; protein folding; response to unfolded protein
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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