Granzymes B (GZMS-B) ELISA Kit for Human

Name: Human GZMS-B ELISA Kit; Granzymes B; MyBioSource
Brand: MyBioSource
SKU: MBS042370
Price: 675 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS042370

Background/Introduction: This Quantitative Sandwich ELISA kit is only for in vitro research use only! It is intended to be determinated GZMS-B concentrations in Human serum, plasma and other body fluids. Using Purified Human GZMS-B antibody to coat Microelisa… More details

$675 / 48-Strip-Wells +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

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Product Overview

Online Datasheet in PDF

Product Name

Granzymes B (GZMS-B), ELISA Kit

Full Product Name

Human Granzymes B ELISA Kit

Product Gene Name

GZMS-B elisa kit

Species Reactivity

Human

Assay Type

Sandwich

Samples

Serum, Plasma, Tissue Homogenate, Feces, Urine and Body Fluids

Detection Range

3.12pg/ml - 100pg/ml.

Sensitivity

1.0pg/ml.

Specificity

No significant cross-reactivity or interference between Human GZMS-B and analogues was observed.

Preparation and Storage

Store all reagents at 2-8 degree C

Inter-assay Precision

Inter-assay CV (%) is less than 15%. [CV(%) = SD/mean x100]

Intra-assay Precision

Intra-assay CV (%) is less than 15%.

Molecular Weight

27,716 Da

Chromosome Location

Chromosome: 14; NC_000014.8 (25100160..25103432, complement). Location: 14q11.2

OMIM

123910

3D Structure

ModBase 3D Structure for P10144

Intended Uses

This Quantitative Sandwich ELISA kit is only for in vitro research use only, not for drug, household, therapeutic applications! It is intended to be determinated GZMS-B concentrations in Human serum, plasma and other body fluids. Using Purified Human GZMS-B antibody to coat Microelisa Stripplate wells to make solid-phase antibody, then add GZMS-B and GZMS-B antibody which has been labeled with HRP to wells, then the reactants become antibody-antigen-antibody-enzyme complex, after washing completely, add TMB substrate solution, TMB substrate becomes blue color under HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of GZMS-B in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Other Notes

Small volumes of GZMS-B elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for GZMS-B purchase

MBS042370 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Granzymes B (GZMS-B) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing GZMS-B. The ELISA analytical biochemical technique of the MBS042370 kit is based on GZMS-B antibody-GZMS-B antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect GZMS-B antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, GZMS-B. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).

Additional Details

NCBI/Uniprot data below describe general gene information for GZMS-B. It may not necessarily be applicable to this product.

NCBI GI #

221625528

NCBI GeneID

3002

NCBI Accession #

NP_004122.2

UniProt Primary Accession #

P10144

UniProt Secondary Accession #

Q8N1D2; Q9UCC1

UniProt Related Accession #

P10144

GenBank Nucleotide #

NM_004131.4

NCBI Official Full Name

granzyme B

NCBI Official Synonym Full Names

granzyme B (granzyme 2, cytotoxic T-lymphocyte-associated serine esterase 1)

NCBI Official Symbol

GZMB

NCBI Official Synonym Symbols

HLP; CCPI; CGL1; CSPB; SECT; CGL-1; CSP-B; CTLA1; CTSGL1

UniProt Protein Name

Granzyme B

UniProt Synonym Protein Names

C11; CTLA-1; Cathepsin G-like 1; CTSGL1; Cytotoxic T-lymphocyte proteinase 2; Lymphocyte protease; Fragmentin-2; Granzyme-2; Human lymphocyte protein; HLP; SECT; T-cell serine protease 1-3E

UniProt Gene Name

GZMB

UniProt Synonym Gene Names

CGL1; CSPB; CTLA1; GRB; CTSGL1; Lymphocyte protease; HLP

UniProt Entry Name

GRAB_HUMAN

NCBI Protein Information

granzyme B; C11; CTLA-1; fragmentin 2; fragmentin-2; cathepsin G-like 1; human lymphocyte protein; T-cell serine protease 1-3E; cytotoxic serine protease B; cytotoxic T-lymphocyte proteinase 2

NCBI Summary for GZMS-B

Cytolytic T lymphocytes (CTL) and natural killer (NK) cells share the remarkable ability to recognize, bind, and lyse specific target cells. They are thought to protect their host by lysing cells bearing on their surface 'nonself' antigens, usually peptides or proteins resulting from infection by intracellular pathogens. The protein encoded by this gene is crucial for the rapid induction of target cell apoptosis by CTL in cell-mediated immune response. [provided by RefSeq, Jul 2008]

UniProt Comments for GZMS-B

GZMB: This enzyme is necessary for target cell lysis in cell- mediated immune responses. It cleaves after Asp. Seems to be linked to an activation cascade of caspases (aspartate-specific cysteine proteases) responsible for apoptosis execution. Cleaves caspase-3, -7, -9 and 10 to give rise to active enzymes mediating apoptosis. By staphylococcal enterotoxin A (SEA) in peripheral blood leukocytes. Inactivated by the serine protease inhibitor diisopropylfluorophosphate. Belongs to the peptidase S1 family. Granzyme subfamily.

Protein type: Protease; Apoptosis; EC 3.4.21.79

Chromosomal Location of Human Ortholog: 14q11.2

Cellular Component: membrane; intracellular membrane-bound organelle; cytoplasm; immunological synapse; cytosol; nucleus

Molecular Function: protein binding; serine-type peptidase activity; serine-type endopeptidase activity

Biological Process: Notch signaling pathway; induction of apoptosis by granzyme; natural killer cell mediated cytotoxicity; apoptosis; cytolysis; immune response; protein processing; proteolysis

Research Articles on GZMS-B

1. Granzyme B cleavage of fibronectin disrupts endothelial cell adhesion, migration and capillary tube formation.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with GZMS-B elisa kit

Reference Product	PubMed Publications
PRF1 elisa kit	>526 publications with GZMS-B and PRF1
CASP3 elisa kit	>87 publications with GZMS-B and CASP3
BID elisa kit	>41 publications with GZMS-B and BID
SERPINB9 elisa kit	>36 publications with GZMS-B and SERPINB9
CASP7 elisa kit	>13 publications with GZMS-B and CASP7
IL2RA elisa kit	>9 publications with GZMS-B and IL2RA
IL2RB elisa kit	>5 publications with GZMS-B and IL2RB
IL2 elisa kit	>2 publications with GZMS-B and IL2

Pathways associated with GZMS-B elisa kit

Products by Pathway	Pathway Diagram
Activation, Myristolyation Of BID And Translocation To Mitochondria Pathway antibodies	Activation, Myristolyation Of BID And Translocation To Mitochondria Pathway Diagram
Allograft Rejection Pathway antibodies	Allograft Rejection Pathway Diagram
Allograft Rejection Pathway antibodies	Allograft Rejection Pathway Diagram
Apoptosis Pathway antibodies	Apoptosis Pathway Diagram
Apoptosis Pathway antibodies	Apoptosis Pathway Diagram
Autoimmune Thyroid Disease Pathway antibodies	Autoimmune Thyroid Disease Pathway Diagram
Autoimmune Thyroid Disease Pathway antibodies	Autoimmune Thyroid Disease Pathway Diagram
Caspase Cascade In Apoptosis Pathway antibodies	Caspase Cascade In Apoptosis Pathway Diagram
Downstream Signaling In Naive CD8+ T Cells Pathway antibodies	Downstream Signaling In Naive CD8+ T Cells Pathway Diagram
Graft-versus-host Disease Pathway antibodies	Graft-versus-host Disease Pathway Diagram

Diseases associated with GZMS-B elisa kit

Disease Name	Pubmed Publications
Neoplasms Antibodies	>227 publications with GZMS-B and Neoplasms
Necrosis Antibodies	>122 publications with GZMS-B and Necrosis
Inflammation Antibodies	>51 publications with GZMS-B and Inflammation
Neoplasms, Experimental Antibodies	>35 publications with GZMS-B and Neoplasms, Experimental
Disease Models, Animal Antibodies	>35 publications with GZMS-B and Disease Models, Animal
Nervous System Diseases Antibodies	>31 publications with GZMS-B and Nervous System Diseases
Liver Diseases Antibodies	>29 publications with GZMS-B and Liver Diseases
Hypersensitivity Antibodies	>24 publications with GZMS-B and Hypersensitivity
Breast Neoplasms Antibodies	>16 publications with GZMS-B and Breast Neoplasms
Hepatitis Antibodies	>15 publications with GZMS-B and Hepatitis

Organs/Tissues associated with GZMS-B elisa kit

Organ/Tissue Name	Pubmed Publications
Blood Antibodies	>838 publications with GZMS-B and Blood
Kidney Antibodies	>82 publications with GZMS-B and Kidney
Lung Antibodies	>67 publications with GZMS-B and Lung
Liver Antibodies	>65 publications with GZMS-B and Liver
Spleen Antibodies	>63 publications with GZMS-B and Spleen
Bone Marrow Antibodies	>48 publications with GZMS-B and Bone Marrow
Heart Antibodies	>35 publications with GZMS-B and Heart
Intestine Antibodies	>28 publications with GZMS-B and Intestine
Pancreas Antibodies	>14 publications with GZMS-B and Pancreas
Stomach Antibodies	>10 publications with GZMS-B and Stomach