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LYP/PTPN22 (PTPN22) Antibody

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS423705

MBS423705 is an antibody targeting PTPN22. Also known as Goat anti-LYP/PTPN22 Antibody. Reactive in HumanExpected from sequence similarity: Chimpanzee. More details

$450 / 0.1 mg +1 FREE 8GB USB
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Product Overview

Online Datasheet in PDF
Product Name
LYP/PTPN22, Antibody
Full Product Name
Goat anti-LYP/PTPN22 Antibody
Product Gene Name
anti-PTPN22 antibody
Product Synonym Names
PTPN22; LYP; Lyp1; Lyp2; protein tyrosine phosphatase, non-receptor type 22 (lymphoid); protein tyrosine phosphatase homolog; lymphoid-specific protein tyrosine phosphatase
Species Reactivity
Human
Expected from sequence similarity: Chimpanzee
Form/Format
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Purity/Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Specificity
This antibody is specific for human LYP1 and will not cross-react with LYP2.
Preparation and Storage
Aliquot and store at -20 degree C. Minimize freezing and thawing.
Antibody/Peptide Pairs
PTPN22 peptide (MBS428772) is used for blocking the activity of LYP/PTPN22 antibody (MBS423705)
Molecular Weight
75,488 Da
OMIM
152700
3D Structure
ModBase 3D Structure for Q9Y2R2
Peptide Sequence + C
CPPNKPAESVQSNNS
Epitope
Internal Region
ISO Certification
Manufactured in an ISO 9001:2008 Certified Laboratory.
Applications Tested/Suitable for anti-PTPN22 antibody
Peptide ELISA (EIA), Flow Cytometry (FC/FACS), Immunofluorescence (IF), Immunohistochemistry (IHC) Paraffin
Application Notes for anti-PTPN22 antibody
Pep-ELISA: antibody detection limit dilution 1:32000.
WB: Preliminary experiments showed a 100kDa band in some Daudi cell lysates at 0.5-1ug/ml. An additional band at 75kDa was also observed and both bands were successfully blocked by incubation with the immunizing peptide.
IHC: Paraffin embedded Human Small Intestine, Thyroid and Adrenal Gland. Recommended concentration: 3.75ug/ml.
IF: Strong expression of the protein seen in the nucleus of U2OS cells. Recommended concentration: 10ug/ml.
Flow Cytometry: Flow cytometric analysis of Jurkat cells. Recommended concentration: 10ug/ml.

Immunofluorescence (IF) of anti-PTPN22 antibody

Immunofluorescence analysis of paraformaldehyde fixed U2OS cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing nuclear staining. Actin filaments were stained with phalloidin (red). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).
Anti-PTPN22 Antibody – Cat# MBS423705

Flow Cytometry (FC/FACS) of anti-PTPN22 antibody

Flow cytometric analysis of paraformaldehyde fixed Jurkat cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (1ug/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.
Anti-PTPN22 Antibody, Flow Cytometry (FC/FACS) – Cat# MBS423705

Immunohistochemistry (IHC) of anti-PTPN22 antibody

(3.75ug/ml) staining of paraffin embedded Human Small Intestine. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
Anti-PTPN22 Antibody, Immunohistochemistry (IHC) – Cat# MBS423705

Immunohistochemistry (IHC) of anti-PTPN22 antibody

(3.75ug/ml) staining of paraffin embedded Human Thyroid. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
Anti-PTPN22 Antibody, Immunohistochemistry (IHC) – Cat# MBS423705

Other Notes

Small volumes of anti-PTPN22 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for PTPN22. It may not necessarily be applicable to this product.

NCBI GI #
224586929
NCBI GeneID
26191
NCBI Accession #
NP_057051
NCBI Related Accession #
NP_036543.4; NP_001180360.1; NP_001295226.1
UniProt Primary Accession #
Q9Y2R2
UniProt Secondary Accession #
O95063; O95064; Q6IPX8; Q8WVM1; A0N0K6; B1ALC8; D4NZ71; E9PLD8; E9PPI1
UniProt Related Accession #
Q9Y2R2
GenBank Nucleotide #
NM_015967.6
NCBI Official Full Name
tyrosine-protein phosphatase non-receptor type 22 isoform 1
NCBI Official Synonym Full Names
protein tyrosine phosphatase, non-receptor type 22
NCBI Official Symbol
PTPN22
NCBI Official Synonym Symbols
LYP; PEP; LYP1; LYP2; PTPN8; PTPN22.5; PTPN22.6
UniProt Protein Name
Tyrosine-protein phosphatase non-receptor type 22
UniProt Synonym Protein Names
Hematopoietic cell protein-tyrosine phosphatase 70Z-PEP; Lymphoid phosphatase; LyP; PEST-domain phosphatase; PEP
Protein Family
Tyrosine-protein phosphatase non-receptor
UniProt Gene Name
PTPN22
UniProt Synonym Gene Names
PTPN8; LyP; PEP
NCBI Protein Information
tyrosine-protein phosphatase non-receptor type 22

NCBI Summary for PTPN22

This gene encodes of member of the non-receptor class 4 subfamily of the protein-tyrosine phosphatase family. The encoded protein is a lymphoid-specific intracellular phosphatase that associates with the molecular adapter protein CBL and may be involved in regulating CBL function in the T-cell receptor signaling pathway. Mutations in this gene may be associated with a range of autoimmune disorders including Type 1 Diabetes, rheumatoid arthritis, systemic lupus erythematosus and Graves' disease. Alternatively spliced transcript variants encoding distinct isoforms have been described. [provided by RefSeq, Mar 2009]
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UniProt Comments for PTPN22

Acts as negative regulator of T-cell receptor (TCR) signaling by direct dephosphorylation of the Src family kinases LCK and FYN, ITAMs of the TCRz/CD3 complex, as well as ZAP70, VAV, VCP and other key signaling molecules (PubMed:16461343, PubMed:18056643). Associates with and probably dephosphorylates CBL. Dephosphorylates LCK at its activating 'Tyr-394' residue (PubMed:21719704). Dephosphorylates ZAP70 at its activating 'Tyr-493' residue (PubMed:16461343). Dephosphorylates the immune system activator SKAP2 (PubMed:21719704). Positively regulates toll-like receptor (TLR)-induced type 1 interferon production (PubMed:23871208). Promotes host antiviral responses mediated by type 1 interferon (). Regulates NOD2-induced pro-inflammatory cytokine secretion and autophagy (PubMed:23991106).
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Research Articles on PTPN22

1. A higher prevalence of homozygous PTPN22 -1123CC genotype was found in controls than in rheumatoid arthritis (RA) patients, suggesting a potential protective effect against RA. Concerning anti-cyclic citrullinated protein antibodies levels, the CC genotype carriers showed the lowest median levels in RA.
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-PTPN22 antibody

 Reference Product  PubMed Publications
 HLA-DRB1 antibody  >130 publications with PTPN22 and HLA-DRB1
 CD4 antibody  >30 publications with PTPN22 and CD4
 CSK antibody  >27 publications with PTPN22 and CSK
 LCK antibody  >8 publications with PTPN22 and LCK
 HLA-DPB1 antibody  >7 publications with PTPN22 and HLA-DPB1
 ZAP70 antibody  >7 publications with PTPN22 and ZAP70
 HLA-DRA antibody  >4 publications with PTPN22 and HLA-DRA

Pathways associated with anti-PTPN22 antibody

 Products by Pathway  Pathway Diagram
 Adaptive Immune System Pathway antibodies  Adaptive Immune System Pathway Diagram
 Immune System Pathway antibodies  Immune System Pathway Diagram
 Phosphorylation Of CD3 And TCR Zeta Chains Pathway antibodies  Phosphorylation Of CD3 And TCR Zeta Chains Pathway Diagram
 TCR Signaling Pathway antibodies  TCR Signaling Pathway Diagram
 Translocation Of ZAP-70 To Immunological Synapse Pathway antibodies  Translocation Of ZAP-70 To Immunological Synapse Pathway Diagram

Diseases associated with anti-PTPN22 antibody

 Disease Name  Pubmed Publications
 Autoimmune Diseases Antibodies  >729 publications with PTPN22 and Autoimmune Diseases
 Immune System Diseases Antibodies  >652 publications with PTPN22 and Immune System Diseases
 Arthritis, Rheumatoid Antibodies  >326 publications with PTPN22 and Arthritis, Rheumatoid
 Diabetes Mellitus, Type 1 Antibodies  >190 publications with PTPN22 and Diabetes Mellitus, Type 1
 Lupus Erythematosus, Systemic Antibodies  >115 publications with PTPN22 and Lupus Erythematosus, Systemic
 Graves Disease Antibodies  >71 publications with PTPN22 and Graves Disease
 Inflammation Antibodies  >66 publications with PTPN22 and Inflammation
 Necrosis Antibodies  >40 publications with PTPN22 and Necrosis
 Inflammatory Bowel Diseases Antibodies  >35 publications with PTPN22 and Inflammatory Bowel Diseases
 Disease Models, Animal Antibodies  >32 publications with PTPN22 and Disease Models, Animal

Organs/Tissues associated with anti-PTPN22 antibody

 Organ/Tissue Name  Pubmed Publications
 Blood Antibodies  >259 publications with PTPN22 and Blood
 Thyroid Antibodies  >82 publications with PTPN22 and Thyroid
 Pancreas Antibodies  >29 publications with PTPN22 and Pancreas
 Skin Antibodies  >26 publications with PTPN22 and Skin
 Thymus Antibodies  >20 publications with PTPN22 and Thymus
 Bone Marrow Antibodies  >13 publications with PTPN22 and Bone Marrow
 Muscle Antibodies  >10 publications with PTPN22 and Muscle
 Lymph Node Antibodies  >9 publications with PTPN22 and Lymph Node
 Lymph Antibodies  >7 publications with PTPN22 and Lymph
 Spleen Antibodies  >6 publications with PTPN22 and Spleen
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