Biotinylated (0.1ug/ml) staining of Mouse Testis lysate (35ug protein in RIPA buffer), exactly mirroring its parental non-biotinylated product. Primary incubation was 1 hour. Detected by chemiluminescence, using streptavidin-HRP and using NAP blocker as a substitute for skimmed milk.

CTDSP1 Antibody

Name: CTDSP1 Antibody; Rat-Reactive; Rat-Reactive; MyBioSource
Brand: MyBioSource
SKU: MBS423708
Price: 450 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS423708

MBS423708 is an antibody targeting CTDSP1. Also known as Goat anti-CTDSP1, Biotinylated Antibody. Reactive in Human, MouseExpected from sequence similarity: Rat, Dog, Cow. More details

$450 / 0.1 mg +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Online Datasheet in PDF

Product Name

CTDSP1, Antibody

Full Product Name

Goat anti-CTDSP1, Biotinylated Antibody

Product Gene Name

anti-CTDSP1 antibody

Product Synonym Names

Goat Anti-CTDSP1 Antibody; CTDSP1; CTD small phosphatase 1; NIF3; NLI-IF; NLIIF; SCP1; CTD (carboxy-terminal domain, RNA polymerase II, polypeptide A) small phosphatase 1; NLI-interacting factor 3; nuclear LIM interactor-interacting factor 3; small C-terminal domain phosphatase 1

Species Reactivity

Human, Mouse
Expected from sequence similarity: Rat, Dog, Cow

Form/Format

Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

Purity/Purification

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Preparation and Storage

Aliquot and store at -20 degree C. Minimize freezing and thawing.

Matching Pairs

Unconjugated Antibody: CTDSP1 (MBS422893)
Biotin Conjugated Antibody: CTDSP1 (MBS423708)

Antibody/Peptide Pairs

CTDSP1 peptide (MBS428521) is used for blocking the activity of CTDSP1 antibody (MBS423708)

Molecular Weight

29.3kDa

OMIM

605323

3D Structure

ModBase 3D Structure for Q9GZU7

Immunogen Sequence

Peptide with sequence EAKAQDSDKIC., from the internal region of the protein sequence according to NP_067021.1; NP_872580.1; NP_001193807.1.

Peptide Sequence + C

EAKAQDSDKIC.

Epitope

Internal Region

Note

This antibody is expected to recognize all three reported isoforms (NP_067021.1; NP_872580.1; NP_001193807.1).

ISO Certification

Manufactured in an ISO 9001:2008 Certified Laboratory.

Applications Tested/Suitable for anti-CTDSP1 antibody

Peptide ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC)

Application Notes for anti-CTDSP1 antibody

Pep-ELISA: antibody detection limit dilution 1:16000.
WB: Approx 30kDa band observed in Mouse Testis lysates. Recommended concentration: 0.1-0.3ug/ml.

Other Notes

Small volumes of anti-CTDSP1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for CTDSP1. It may not necessarily be applicable to this product.

NCBI GI #

10864009

NCBI GeneID

58190

NCBI Accession #

NP_067021.1

NCBI Related Accession #

NP_872580.1; NP_001193807.1; NP_694728.1

UniProt Primary Accession #

Q9GZU7

UniProt Secondary Accession #

Q7Z5Q3; Q7Z5Q4; C9IYG0

UniProt Related Accession #

Q9GZU7

GenBank Nucleotide #

NM_021198.2

NCBI Official Full Name

carboxy-terminal domain RNA polymerase II polypeptide A small phosphatase 1 isoform 1

NCBI Official Synonym Full Names

CTD small phosphatase 1

NCBI Official Symbol

CTDSP1

NCBI Official Synonym Symbols

NIF3; SCP1; NLIIF; NLI-IF

UniProt Protein Name

Carboxy-terminal domain RNA polymerase II polypeptide A small phosphatase 1

UniProt Synonym Protein Names

Nuclear LIM interactor-interacting factor 3; NLI-IF; NLI-interacting factor 3; Small C-terminal domain phosphatase 1; SCP1; Small CTD phosphatase 1

Protein Family

Carboxy-terminal domain RNA polymerase II polypeptide A small phosphatase

UniProt Gene Name

CTDSP1

UniProt Synonym Gene Names

NIF3; NLIIF; SCP1; NLI-IF; NLI-interacting factor 3; SCP1; Small CTD phosphatase 1

NCBI Protein Information

carboxy-terminal domain RNA polymerase II polypeptide A small phosphatase 1

NCBI Summary for CTDSP1

This gene encodes a member of the small C-terminal domain phosphatase (SCP) family of nuclear phosphatases. These proteins play a role in transcriptional regulation through specific dephosphorylation of phosphoserine 5 within tandem heptapeptide repeats of the C-terminal domain of RNA polymerase II. The encoded protein plays a role in neuronal gene silencing in non-neuronal cells, and may also inhibit osteoblast differentiation. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Oct 2011]

UniProt Comments for CTDSP1

Preferentially catalyzes the dephosphorylation of 'Ser-5' within the tandem 7 residue repeats in the C-terminal domain (CTD) of the largest RNA polymerase II subunit POLR2A. Negatively regulates RNA polymerase II transcription, possibly by controlling the transition from initiation/capping to processive transcript elongation. Recruited by REST to neuronal genes that contain RE-1 elements, leading to neuronal gene silencing in non-neuronal cells.

Research Articles on CTDSP1

1. Our results provide a novel mechanistic insight into the activation of Myc by 27-HC via transcriptional repression of PP2A, SCP1 and FBW7 to increase Myc protein stability in breast cancer cells.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-CTDSP1 antibody

Reference Product	PubMed Publications
REST antibody	>4 publications with CTDSP1 and REST
CTDSPL antibody	>2 publications with CTDSP1 and CTDSPL
CDCA3 antibody	>1 publications with CTDSP1 and CDCA3
CTDP1 antibody	>1 publications with CTDSP1 and CTDP1
SMAD3 antibody	>1 publications with CTDSP1 and SMAD3
SMAD2 antibody	>1 publications with CTDSP1 and SMAD2
SMAD1 antibody	>1 publications with CTDSP1 and SMAD1

Pathways associated with anti-CTDSP1 antibody

Products by Pathway	Pathway Diagram
BMP Receptor Signaling Pathway antibodies	BMP Receptor Signaling Pathway Diagram
Coregulation Of Androgen Receptor Activity Pathway antibodies	Coregulation Of Androgen Receptor Activity Pathway Diagram
Regulation Of Cytoplasmic And Nuclear SMAD2/3 Signaling Pathway antibodies	Regulation Of Cytoplasmic And Nuclear SMAD2/3 Signaling Pathway Diagram
Mir-124 Predicted Interactions With Cell Cycle And Differentiation Pathway antibodies	Mir-124 Predicted Interactions With Cell Cycle And Differentiation Pathway Diagram

Diseases associated with anti-CTDSP1 antibody

Disease Name	Pubmed Publications
Infertility, Male Antibodies	>3 publications with CTDSP1 and Infertility, Male
Liver Neoplasms Antibodies	>2 publications with CTDSP1 and Liver Neoplasms
Necrosis Antibodies	>2 publications with CTDSP1 and Necrosis
Death Antibodies	>1 publications with CTDSP1 and Death
Carcinoma, Hepatocellular Antibodies	>1 publications with CTDSP1 and Carcinoma, Hepatocellular
Oligospermia Antibodies	>1 publications with CTDSP1 and Oligospermia
Adenocarcinoma Antibodies	>1 publications with CTDSP1 and Adenocarcinoma

Organs/Tissues associated with anti-CTDSP1 antibody

Organ/Tissue Name	Pubmed Publications
Brain Antibodies	>4 publications with CTDSP1 and Brain
Nerve Antibodies	>3 publications with CTDSP1 and Nerve
Liver Antibodies	>3 publications with CTDSP1 and Liver
Blood Antibodies	>3 publications with CTDSP1 and Blood
Bone Antibodies	>1 publications with CTDSP1 and Bone
Testis Antibodies	>1 publications with CTDSP1 and Testis
Lymph Node Antibodies	>1 publications with CTDSP1 and Lymph Node
Prostate Antibodies	>1 publications with CTDSP1 and Prostate
Vascular Antibodies	>1 publications with CTDSP1 and Vascular
Lymph Antibodies	>1 publications with CTDSP1 and Lymph