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NODAL Antibody

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS423726

MBS423726 is an antibody targeting NODAL. Also known as Goat anti-NODAL Antibody. Reactive in RatExpected from sequence similarity: Human, Mouse. More details

$450 / 0.1 mg +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
NODAL, Antibody
Full Product Name
Goat anti-NODAL Antibody
Product Gene Name
Product Synonym Names
NODAL, nodal growth differentiation factor, HTX5
Species Reactivity
Rat
Expected from sequence similarity: Human, Mouse
Form/Format
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Purity/Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Preparation and Storage
Aliquot and store at -20 degree C. Minimize freezing and thawing.
Antibody/Peptide Pairs
NODAL peptide (MBS428784) is used for blocking the activity of NODAL antibody (MBS423726)
Molecular Weight
40.5kDa
OMIM
270100
Immunogen Sequence
Peptide with sequence DRSQLCRKVKFQ, from the internal region of the protein sequence according to NP_060525.2.
Peptide Sequence + C
DRSQLCRKVKFQ
Epitope
Internal Region
ISO Certification
Manufactured in an ISO 9001:2008 Certified Laboratory.
Applications Tested/Suitable for anti-NODAL antibody
Peptide ELISA (EIA), Western Blot (WB)
Application Notes for anti-NODAL antibody
Pep-ELISA: antibody detection limit dilution 1:64000.
WB: Approx 40kDa band observed in Rat Brain lysates. Recommended concentration: 0.01-0.1ug/ml.

Immunohistochemistry (IHC) of anti-NODAL antibody

(0.03ug/ml) staining of Rat Brain lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Anti-NODAL Antibody – Cat# MBS423726

Other Notes

Small volumes of anti-NODAL antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for NODAL. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI Related Accession #
UniProt Secondary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
nodal
NCBI Official Synonym Full Names
nodal growth differentiation factor
NCBI Official Symbol
NODAL
NCBI Official Synonym Symbols
HTX5
UniProt Protein Name
Nodal homolog
Protein Family
UniProt Gene Name
NODAL
NCBI Protein Information
nodal homolog

NCBI Summary for NODAL

This gene encodes a secreted ligand of the TGF-beta (transforming growth factor-beta) superfamily of proteins. Ligands of this family bind various TGF-beta receptors leading to recruitment and activation of SMAD family transcription factors that regulate gene expression. The encoded preproprotein is proteolytically processed to generate the mature protein, which regulates early embryonic development. This protein is required for maintenance of human embryonic stem cell pluripotency and may play a role in human placental development. Mutations in this gene are associated with heterotaxy, a condition characterized by random orientation of visceral organs with respect to the left-right axis. [provided by RefSeq, Aug 2016]
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UniProt Comments for NODAL

Essential for mesoderm formation and axial patterning during embryonic development.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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